Streptomycetes are industrial producers of antibiotics, enzymes, vitamins. When streptomycetes are cultivated in the liquid medium of bioreactors, their mycelium forms pellets. Due to the influence of hyphae aggregation on the efficiency of microbiological production, the study of the influence of genetic determination of pellets formation in deep cultivation is an urgent task. The aim of the work is to determine the presence of genes in the genome of wild type strain S. globisporus 1912, whose products are necessary for the aggregation of hyphae into pellets. We analyzed the genomic sequences of 2 mutants (1912-4Crt and 1912-2) of S. globisporus 1912 strain. The 1912-4Crt variant is a spontaneous mutant of the original strain that began to produce carotenoids. The mutation of variant 1912-2 led to decrease in landomycin E synthesis. The genomic DNA sequence of S. globisporus 1912-4Crt is available in the GenBank database (accession QWFA00000000.1). The primary structure of the genomic DNA of variant 1912-2 is not placed in National Center for Biotechnology Information (NCBI) databases. Sequences alignment was performed by BLASTN programs of the NCBI server. Streptomycete mycelium was grown in Okanishi’s medium with stirring at a temperature of 28 °C for 3 days. During cultivation of the wild type strain S. globisporus 1912 and its mutants in a liquid medium, their mycelium forms pellets, so the presence of at least one of the indicated clusters in the chromosome was expected. Using BLASTN analysis in genomes of 2 S. globisporus 1912 variants, sequences similar to the structures of both mycelial aggregation clusters of the most researched strains S. coelicolor A3(2) and S. griseus NBRC 13350 were found. However, if the mat-cluster was represented in one cluster per chromosome of the mutant, then two cslA/glxA/dtpA-clusters were detected in the chromosomes of both variants (1912-4Crt and 1912-2). Analysis of genetic map of the chromosome of variant 1912-4Crt revealed that its 2 cslA/glxA/dtpA clusters are organized according to different schemes — one of the clusters contains an insertion in 2 genes. The revealed difference in the organization of the cslA/glxA/dtpA clusters of the strain S. globisporus 1912 is of particular importance against background of the fact that similar clusters in genome of related strain S. griseus NBRC 13350 have the same organization scheme, while in the genome of S. coelicolor A3(2) only one similar cluster is present.
Keywords: streptomycete, pellet, nucleotide sequence, cluster, gene, similarity indicators
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