Phosphorus is a key mineral for a human as well as an animal body. Approximately 70-80 % of total seed phosphorus content of cereals and legumes are stored in a bounded form of phytic acid (mio-inositol-1,2,3,4,5,6-hexa-kisphosphate) and therefore is not available for human and non-ruminant animals’ nutrition. Inaccessible to digestion organic phosphorus in form of phytates excreted from the body of non-ruminant animals and poultry with feces creates an ecological problem displayed as drinking water pollution (eutrophication of waterways). Development of low-phytate hull-less barley varieties on the base of lpa-mutations allows to enhance substantially the grain phosphorus uptake (bioavailability) by animals and humans thereby decreasing the harmful ecological load with organic phosphates. Series of original lpa-mutations was used in a special breeding program aimed on development of low-phytate hull-less barley with grain enhanced mineral phosphorus in combination with black seed pericarp (from Abyssinian 1105 collection strain) as a marker of increased anthocyanin pigments content possessing with elevated grain antioxidant activity. As a lpa-mutations recipient spring hull-less barley commercial variety Achilles was used. On the base of crosses several segregating populations were developed. The number of advanced breeding hull-less barley lines with elevated grain mineral phosphorus combined with black pericarp were isolated. Laboratory protocol needed for detection of lpa-mutations in breeding populations was used with some modifications and improvements required for efficient selection of the target lpa-genotypes. Laboratory procedure sensitivity allows reliable detection of lpa-mutations in breeding population using minimal sample size as single individual seeds or parts of them.
Keywords: hull-less barley, phytates, mineral phosphorus, lpa-mutations, biofortification
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