The aim of the study was to develop an effective method of in vitro cultivation of cherry cultivar Gisela 5 (Prunus cerasus ´ Prunus canescens), which is a valuable rootstock variety in the climatic conditions of Ukraine, using the method of adding liquid nutrient medium at the stage of elongation and further rooting stage. The effect of adding liquid nutrient medium (Quorin & Lepoivre — QL) with gibberellic acid (GA3) at three concentrations (0.5 mg/l, 1.0 mg/l and 1.5 mg/l) when added on the third week of cultivation on the medium for multiplication was studied with subsequent cultivation for two weeks. Also, the effect of pre-addition of liquid nutrient medium on the effectiveness of subsequent rooting was investigated. It was shown that the addition of 5 ml of liquid nutrient medium with 1 mg/l GA3 was the most effective, with a high number of plants obtained for rooting. In the case of GA3 1.5 mg/l the number of shoots with necrotic manifestations and signs of hyperhydration (vitrification) increased, such shoots are unsuitable for further rooting or multiplication. It was supposed that the reason for this was the increase in humidity in the container with plants, and the high concentration of GA3. The dependence of rooting on the previous addition of liquid nutrient medium with GA3 was evaluated, with an increase in total roots, root length and total number of rooted plants compared to the control, which in turn will improve further adaptation of plants to ex vitro conditions, but a clear dependence on concentration GA3 was not observed.
Keywords: Gisela 5 cherry rootstock, in vitro, liquid nutrient media, elongation, multiplication, rooting, plant growth regulators, gibberellic acid
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1. Druart, P. (2013). Micropropagation of prunus species relevant to cherry fruit production. Protocols for micropropagation of selected economically important horticultural plants. Methods Mol. Biol., 994, pp. 119-136. https://doi.org/10.1007/978-1-62703-074-8_9
2. Long, L.E. & Kaiser, C. (2010). Sweet cherry rootstocks for the Pacific Northwest. A Pacific Northwest Extension Publication, PNW 619, Oregon State University.
3. Long, L.E. (2003). Cherry training systems, selection and development, PNW 543, Corvallis, Oregon State University.
4. Erwin, D.C. & Ribeiro, O.K. (1996). Phytophthora diseases worldwide. APS Press, American Phytopathological Society, St. Paul, MN, USA.
5. Spahiє, A., Spahiє, A. & Begiє-Akagiє, A. (2012). Testing of 'Gisela 5' and 'Santa Lucia 64' cherry rootstocks in Bosnia and Herzegovina. Acta Agriculturae Slovenica, 99, No. 2, pp. 129-136. https://doi.org/10.2478/v10014-012-0012-5
6. ћiлko, M. (2011). In vitro propagation of 'Gisela 5' (Prunus cerasus ' P. canescens). Agricultura, 8, No. 1, pp. 31-34.
7. Zimmermann, A. (1994). 'Gisela 5', a dwarfing rootstock for sweet cherries from Giessen in a trial. Obstbau (Germany), 19, pp. 62-63.
8. Quorin, M., & Lepoivre, P. (1977). ‹tude de milieux adaptѕs aux culture de prunus. Acta Hortic., 78, pp. 437-442. https://doi.org/10.17660/ActaHortic.1977.78.54
9. Walkey, D. (1972). Production of apple plantlents from axillary bud meristems. Can. J. Plant Sci., 52, pp. 1085-1087. https://doi.org/10.4141/cjps72-186
10. Kataeva, N. (1986). Peculiarities of micropropagation of apple varieties with declined rooting ability. Agric. Biol., 4, pp. 18-23.
11. Wang, Po-Jen & Hu, Ching yeh (1984). In vitro cloning of the deciduous timber tree Sassafras randaiense. Z. Pflanzenphysiol., 113, pp. 331-335. https://doi.org/10.1016/S0044-328X(84)80039-2
12. Martin, K.P., Zang, C.L., Slater, A. & Madassery, J. (2007). Control of shoot necrosis and plant death during micropropagation of banana and plantains (Musa spp.). Plant Cell Tissue and Organ Culture, 88, pp. 51-59. https://doi.org/10.1007/s11240-006-9177-0
13. Nezami, S.R., Yadollahi, A., Hokmabadi, H., & Eftekhari, M. (2015). Control of shoot tip necrosis and plant death during in vitro multiplication of Pistachio rootstock UCB1 (Pistacia integrima x P. atlantica). J. Nuts, 6, pp. 27-35.
14. Law, D.M. & Hamilton, R.H. (1984). Effects of gibberellic acid on endogenous indole-3-acetic acid and indoleacetyl aspartic acid levels in dwarf pea. Plant Physiol., 75, pp. 255-256. https://doi.org/10.1104/pp.75.1.255
15. Wiszniewska, A. (2016). Rooting response of Prunus domestica L. microshoots in the presence of phytoactive medium supplements. Plant Cell Tissue and Organ Culture, 125, pp. 163-176. https://doi.org/10.1007/s11240-015-0937-6
16. Geiss, G., Gutierrez, L. & Bellini, C. (2018). Adventitious root formation: new insights and perspectives. In: Roberts JA (Ed). Annual plant reviews online, pp 127-156. https://doi.org/10.1002/9781119312994.apr0400
17. Elmongy, M.S., Cao, Y., Zhou, H. & Xia, Y. (2018). Root development enhanced by using indole-3-butyric acid and naphthalene acetic acid and associated biochemical changes of in vitro azalea microshoots. J. Plant Growth Regul., 37, pp. 813-825. https://doi.org/10.1007/s00344-017-9776-5
18. Ali, M., Abbasi, B.H. & Ali, G.S. (2015). Elicitation of antioxidant secondary metabolites with jasmonates and gibberellic acid in cell suspension cultures of Artemisia absinthium L. Plant Cell Tissue and Organ Culture, 120, pp. 1099-1106. https://doi.org/10.1007/s11240-014-0666-2