Fiziol. rast. genet. 2024, vol. 56, no. 2, 151-165, doi: https://doi.org/10.15407/frg2024.02.151

Formation and functioning of soybean symbiosis with nodule bacteriaunder the influense of homologous lectin and fungicides

Omelchuk S.V., Mykhalkiv L.M., Karaushu O.V.

  • Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine 31/17 Vasylkivska St., Kyiv, 03022, Ukraine

The effectiveness of soybean-rhizobial systems formed by a bacterial suspension, containing Bradyrhizobium japonicum RS07 strain and soybean seed lectin, with soybean plants of the DiademaPodillia variety was studied under conditions of pot experiment with a sand culture, after seed treatment with Maxim XL and Standak Top seed protectants. A tendency to increase the plant height was revealed when lectin was added to the bacterial suspension both in the variant without treatment with fungicides and with their use. The growth of above-ground mass in the stage of bean formation was facilitated by the use of lectin against the background of treatment with the fungicide Maxim XL. It was established that at the initial stages of the symbiosis formation, the tested protectants, regardless of the presence of lectin in the inoculation suspension, favorably influenced the nodules formation, while the nodules mass was mainly at the control levels. The use of a homologous lectin had a positive effect on the symbiosis functioning at the initial stages of soybean plant development. At the same time, the treatment of seeds with the fungicide Maxim XL did not have a toxic effect on this process, in contrast to Standak Top, where the nitrogen-fixing activity was lower by 16.6 %. However, in the second half of the soybean vegetation, this index under the Standak Top application exceeded the control plants by 36.8 and 21.8 % at the flowering and bean formation stages, respectively. In all studied treatments, a tendency to increase the grain productivity was revealed. The use of lectin on the background of treatment with fungicides Maxim XL and Standak Top contributed to an increase in the grain weight by 9.6 and 10.0 %, respectively. The obtained results regarding the effect of fungicides and lectin on the formation and functioning of symbiotic systems and soybean productivity confirm the possibility of their use in the cultivation technology of this crop.

Keywords: Glycine max (L.) Merr., Bradyrhizobium japonicum, symbiosis, Maxym XL, Standak Top, nitrogen fixation, nodules, weight, grain productivity

Fiziol. rast. genet.
2024, vol. 56, no. 2, 151-165

Full text and supplemented materials

Free full text: PDF  

References

1. Kazakova, I.V. & Kondratiuk, N.V. (2015). The efficiency of soybean production and the development of the soybean products market in Ukraine and the world. Efektyvna ekonomika, No. 5.

2. UCAB: In 2023, the area sown under cereals in Ukraine will decrease by 45 %, and the gross harvest - by 60 % (2022, December, 16). Yurydychna gazeta.

3. Kots, S.Ya., Morhun, V.V., Patyka, V.F., Datsenko, V.K., Krugova, E.D., Kyrychenko, O.V., Melnikova, N.N. & Mykhalkiv, L.M. (2010). Biological fixation of nitrogen: legume-rhizobial symbiosis. Vol. 1. Kiev: Logos [in Russian].

4. Soybean in crop rotation: the best options. (2021, February, 18). Agronom.

5. Hablak, S. (2023, January, 25). Modern changes in soybean cultivation technology. Agronom.

6. Vozniuk, S.V., Tytova, L.V., Ratushinska, O.V. & Iutynska, G.O. (2016). Formation and functioning of symbiotic systems and rhizosphere microbiocenosis of soybean under various fungicides application. Mikrobiol. zhurn., 78, No. 4, pp. 59-70 [in Ukrainian]. https://doi.org/10.15407/microbiolj78.04.059

7. Alekseenko, N.V. (2010). The effect of antifungal drugs on the effectiveness of rhizobia symbiosis with chickpea plants. Naukovi pratsi Pivdennogo filialu Natsionalnogo universytetu bioresursiv i pryrodokorystuvannia Ukr. Krymskyi agrotehnol. un-t silskogosp. nauky, 130, pp. 203-208 [in Ukrainian]. https://doi.org/10.1541/ieejfms.130.203

8. Kots, S.Ya., Morhun, V.V., Patyka, V.Ph., Malichenko, S.M., Mamenko, P.N., Kiriziy, D.A., Mykhalkiv, L.M., Berehovenko, S.K. & Melnikova, N.N. (2011). Biological fixation of nitrogen: legume-rhizobial symbiosis. Vol. 2. Kiev: Logos [in Russian].

9. Tn, C.M. (1982). Effect of some pesticides on Rhizobium japonicum and pathogens of soybean. Chemosphere, No. 10, pp. 1027-1039. https://doi.org/10.1016/0045-6535(82)90076-5

10. Derevyanskyi, V.P. (2007). Disease prevalence and productivity of soybeans. The effect of microbiological preparations on reducing the intensity of damage to crops. Karantyn i zakhyst roslyn, No. 5, pp. 11-14 [in Ukrainian].

11. Sergienko, V.G. & Mykolaevskyi, V.P. (2014). Soybean diseases monitoring in the forest-steppe of Ukraine. Karantyn i zakhyst roslyn, 10, No. 11, pp. 9-11 [in Ukrainian].

12. Borzenkova, G.A. (2014). Optimization of technology of preseeding treatment and possibility of its combination with inoculation for protection of soya against contamination with seed infection. Zernob. krup. kult., 1, No. 9, pp. 22-30 [in Russian].

13. Santos, A.B. (2021). Effect of fungicides on the symbiosis between Bradyrhizobium strains and peanut. Pesqui Agr. Trop., 51, e69089. https://doi.org/10.1590/1983-40632021v5169089

14. Roman, D.L., Voiculescu, D.I., Filip, M., Ostafe, V. & Isvoran, A. (2021). Effects of triazole fungicides on soil microbiota and on the activities of enzymes found in soil: review. Agricult., 11, No. 9, 893. https://doi.org/10.3390/agriculture11090893

15. Mostoviak, I.I. & Kravchenko, O.V. (2019). Symbiotic apparatus of soya under the application of different types of fungicides and microbial preparation. Tavr. nauk. visn., 108, pp. 72-77 [in Ukrainian]. https://doi.org/10.32851/2226-0099.2019.108.10

16. Mykolaievsky, V.P., Sergienko, V.G. & Tytova, L.V. (2016). Diseases development and productivity of soybean at the seeds treatment by microbial formulations. Ahrobiol., 2, pp. 96-103 [in Ukrainian]. http://nbuv.gov.ua/UJRN/agr_2016_2_18

17. Silva, K., Silva, E.E., Farias, E.D.N.C., Silva, C.J., Albuquerque, C.N.B. & Cardoso, C. (2018). Agronomic efficiency of Bradyrhizobium pre-inoculation in association with chemical treatment of soybean seeds. Afr. J. Agric. Res., 13, No. 14, pp. 726-732. https://doi.org/10.5897/AJAR2018.13016

18. Rodrigues, T.F., Bender, F.R., Sanzovo, A.W.S., Ferreira, E., Nogueira, M.A. & Hungria, M. (2020). Impact of pesticides in properties of Bradyrhizobium spp. and in the symbiotic performance with soybean. World J. Microbiol. Biotechnol., 36, No. 11, pp. 1-16. https://doi.org/10.1007/s11274-020-02949-5

19. Kyrychenko, O.V., Kots, S.Ya., Khrapova, A.V. & Omelchuk, S.V. (2022). Biological activity of soybean seed lectin at the spraying of Glycine max plants against the background of seed treatment with pesticide containing fiprolin, thiophanate -methyl, pyraclostrobin as active substances and rhizobial bacterization. Regul. Mech. Biosyst., 13, No. 2, pp. 12-20. https://doi.org/10.15421/022215

20. Arfaoui, A., Sifi, B., Boudabous, A., Hadrami, I.El. & Cherif, M. (2006). Identification of Rhisobium isolates possessing antagonistic activity against Fusarium oxysporum sp. Ciceris, the causal agent of Fusarium wilt of chickpea. J. Plant Pathol., 88, No. 1, pp. 67-75.

21. Hardy, R.W.F., Holsten, R.D., Jackson, E.K. & Burns, R.C. (1968). The acethylene - ethylene assay for N2 fixation; laboratory and field evaluation. Plant Physiol., 43, pp. 1185-1207. https://doi.org/10.1104/pp.43.8.1185

22. Krykunets, V.M. (1993). Acetylene-reduction method in research of the physiology of legume-rhizobial simbiosis. (1993). Fiziol. biohim. kult. rast, 25, No. 5, pp. 419-430 [in Ukrainian].

23. Vorobey, N.A., Kukol, K.P. & Kots, S.Ya. (2020). Fungicides toxicity assessment on Bradyrhizobium japonicum nodule bacteria in pure culture. Mikrobiol. Zhurn., 82, No. 3, pp. 45-54 [in Ukrainian]. https://doi.org/10.15407/microbiolj82.03.045

24. Omelchuk, S.V., Kyrychenko, O.V. & Zhemojda, A.V. (2022). Realization of symbiotic and productivity potential of soybean-rhizobia systems formed by analytically selected fungicide resistant strains of nodule bacteria under preliminary treatment of seeds with Standak Top. Fiziol. rast. genet., 54, No. 1, pp. 52-64 [in Ukrainian]. https://doi.org/10.15407/frg2022.01.052

25. Kots, S.Ya. & Kukol, K.P. (2021). The effect of pesticides on nodule bacteria in pure culture and on realization of their symbiotic potential. Fiziol. rast. genet., 53, No. 3, pp. 240-261 [in Ukrainian]. https://doi.org/10.15407/frg2021.03.240

26. Veselovska, L.I., Mykhalkiv, L.M & Kots, S.Ya. (2013). The influence of exogenous lectin on the effectivity of Glycine max-Bradyrhizobium japonicum symbiosis under drought conditions. Fiziol. rast. genet., 45, No. 4, pp. 319-326 [in Ukrainian].

27. Kots, S.Ya., Mykhalkiv, L.M., Mamenko, P.M. & Volkogon, M.V. (2011). The study of Alfalfa - Sinorhizobium meliloti symbiosis productivity under different water conditions and the influence of the legume seed lectin. J. Agric. Sci., B1, No. 3, pp. 454-457.

28. Kyrychenko, O.V. (2014). Phytolectins and diazotrophs are the polyfunctional components of the complex biological compositions. Biotechnol. Acta, 7, No. 1, pp. 40-53 [in Ukrainian]. https://doi.org/10.15407/biotech7.01.040

29. Melnykova, N.M., Mykhalkiv, L.M., Mamenko, P.M. & Kots, S.Ya. (2013). The areas of application for plant lectins. Biopolym. Cell, 29, No. 5, pp. 357-366. https://doi.org/10.7124/bc.00082A

30. Pavlyshche, A.V., Mamenko, T.P., Rybachenko, L.I. & Kots, S.Ya. (2018). Influence of fungicides on the formation, functioning and peroxidase activity of root soybean nodules at inoculation by rhizobia, incubated with lectin. Mikrobiol. zhurn., 80, No. 5, pp. 76-89 [in Ukrainian]. https://doi.org/10.15407/microbiolj80.05.076