Fiziol. rast. genet. 2023, vol. 55, no. 5, 450-460, doi:

Acceleration of herbicide aclonifen phytotoxic action by join application with no donor sodium nitroprusside

Ponomareva I.G., Yukhymuk V.V.

  • Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine 31/17 Vasylkivska St., Kyiv, 03022, Ukraine

The effect of NO donor sodium nitroprusside (SNP) on the phytotoxic effect of the carotenoid synthesis inhibitor herbicide aclonifen was investigated. The research was conducted under the greenhouse conditions using oil radish plants as a model of annual dicotyledonous weeds, as well as in field experiments in sunflower crops. In the greenhouse experiment treatment was carried out in phase of two true leaves by spraying plants with a herbicide separately and with the addition of SNP. The dynamics of the phytotoxic effect development was evaluated by the inhibition of the aboveground part of the plants fresh and dry matter accumulation, as well as by the inhibition of the accumulation of photosynthetic pigments in the leaves. In field experiments, the effect of SNP on the phytotoxic effect of aclonifen on cultivated plants and the effectiveness of weed control was evaluated. It was established that addition of SNP can leads to increase of aclonifen inhibitory effect on sensitive plant species at the early stages of the development of herbicide action. At the same time, the final effect of aclonifen in the range of recommended application rates of 0.6—1.2 kg/ha, both on sensitive and resistant plant species, does not change when SNP is added in concentrations of 1, 3 and 5 mM. It was concluded that join application with NO donor can leads only to the acceleration of development, but not to the strengthening of aclonifen phytotoxic effect. On the basis of a comparison of NO donor input on the phytotoxic effect of aclonifen and herbicides from the classes of synthetic auxins, PPO and ALS inhibitors, it was concluded that dependence of herbicide phytotoxic effect on the formation of ROS is a necessary, but not sufficient condition for the sensitivity of herbicide’s action to NO.

Keywords: NO, sodium nitroprusside, herbicides, aclonifen

Fiziol. rast. genet.
2023, vol. 55, no. 5, 450-460

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1. Hancock, J.T. (2020). Nitric oxide signaling in plants. Plants, 9, No. 11, 1550.

2. Verma, N., Tiwari, S., Singh, V.P. & Prasad, S.M. (2020). Nitric oxide in plants: an ancient molecule with new tasks. Plant Growth Reg., 90, pp. 1-13.

3. Parankusam, S., Adimulam, S.S., Bhatnagar-Mathur, P. & Sharma, K.K. (2017). Nitric oxide (NO) in plant heat stress tolerance: current knowledge and perspectives. Front. Plant Sci., e1582.

4. Sami, F., Faizan, M., Faraz, A., Siddiqui, H., Yusuf, M. & Hayat, S. (2018). Nitric oxide-mediated integrative alterations in plant metabolism to confer abiotic stress tolerance, NO crosstalk with phytohormones and NO-mediated post translational modifications in modulating diverse plant stress. Nitric Oxide., 28 (73), pp. 22-38.

5. Karpetz, Yu.V. (2019). Donors of nitric oxide and their application for increase in plant resistance to action of abiotic stressors. Visn. Hark. nac. agrar. univ., Ser. Biol., 48, No. 3, pp. 28-51. [In Ukrainian]

6. Wang, Y., Loake, J.G. & Chu, C. (2013) Cross-talk of nitric oxide and reactive oxygen species in plant programed cell death. Front. Plant Sci. Sec. Plant Physiol., e314.

7. Li, Z.-C., Ren, Q.-W., Guo, Y., Ran, J., Ren, X.-T., Wu, N.-N., Xu, H.-Y., Liu, X. & Liu, J.-Z. (2021). Dual roles of GSNOR1 in cell death and iмmunity in tetraploid Nicotiana tabacum. Front. Plant Sci., e596234.

8. Hung, K.T., Chang, C.J. & Kao, C.H. (2002). Paraquat toxicity is reduced by nitric oxide in rice leaves. J. Plant Physiol., 159, No. 2, pp. 159-166.

9. Singh, H., Singh, N.B., Singh, A., Hussain, I. & Yadav, V. (2017). Physiological and biochemical roles of nitric oxide against toxicity produced by glyphosate herbicide in Pisum sativum. Rus. J. Plant Physiol., 64, No. 4, pp. 518-524.

10. Ferreira, L.C., Cataneo, A.C., Remaeh, L.M., Coriani, N., Fumis, T., Soyza, Y.A., Scavroni, J. & Soares, B.J. (2010). Nitric oxide reduces oxidative stress generated by lactofen in soybean plants. Pesticide Biochem. Physiol., 97, No. 1, pp. 47-54.

11. Sychuk, A.M. (2015). The participation of programmed cell death in the herbicides induced pathogenesis. (Extended abstract of candidate thesis). Institute of Plant Physiology and Genetics, Kyiv, Ukraine [in Ukrainian].

12. Sychuk, A.M., Radchenko, M.P. & Morderer, Y. (2013). The increase of phytotoxic action of graminicide fenoxaprop-p-ethyl by NO donor sodium nitroprusside. Sci. Educat. New Dimension: Nat. Tech. Sci., 1-2, No. 15, pp. 21-22.

13. Dan Hess, F. (2000). Light-dependent herbicides: an overview. Weed Sci., 48, No. 2, pp. 160-170. [0160:LDHAO]2.0.CO;2 [0160:LDHAO]2.0.CO;2

14. Morderer, Ye.Yu., Palanytsya, M.P. & Rodzevich, O.P. (2008). Study of participation of free radical oxidation reactions in the development of phytotoxic effect of graminicides. Fiziol. biochim. kult. rast., 40, No. 1, pp. 56-61 [in Ukrainian].

15. Palanytsya, M.P., Trach, V.V. & Morderer, Ye.Yu. (2009). The generation of reactive oxygen species under the action of graminicides and modificators of their phytotoxicity. Fiziol. biochim. kult. rast., 41, No. 4, pp. 328-334 [in Ukrainian].

16. Ponomareva, I.G., Khandezhyna, M.V. & Radchenko, M.P. (2022). Increase in the phytotoxic effect of protoporphyrinogen oxidase inhibiting herbicide carfentrazone and herbicide synthetic auxin 2,4-D by join use with the NO donor sodium nitroprusside. Fiziol. rast. genet., 54, No. 5, pp. 419-428. [in Ukrainian].

17. Kilinc, љ., Grasset, R. & Reynaud, S. (2011). The herbicide aclonifen: the complex theoretical bases of sunflower tolerance. Pesticide Biochem. Physiology, 100, Iss. 2, pp. 193-198.

18. Kahlau, S., SchrШder, F., Freigang, J., Laber, B., Lange, G., Passon, D., Kleeўen, S., Lohse, M., Schulz, A., Von Koskull-DШring, P., Klie, S. & Gille, S. (2020). Aclonifen targets solanesyl diphosphate synthase, representing a novel mode of action for herbicides. Pest Manag. Sci., 76, No. 10, pp. 3377-3388.

19. Heap, I. (2023). The international herbicide-resistant weed database. Online. Tuesday, October 31, 2023. Retrieved from

20. Welburn, A.R. (1994). The spectral determination of chlorophylls a and b as well as total carotenoids using various solvents with spectrophotometry of different resolution. J. Plant Physiol., 144, No. 3, pp. 307-313.

21. Ivashchenko, O.O. & Merezhinsky, Yu.G. (2001). The effectiveness of herbicides. In Methods of testing and application of pesticides. Tribel, S.O. (Ed.). (pp. 381-383) Kyiv: Svit [in Ukrainian].