The effect of NO donor sodium nitroprusside (SNP) on the phytotoxic effect of the carotenoid synthesis inhibitor herbicide aclonifen was investigated. The research was conducted under the greenhouse conditions using oil radish plants as a model of annual dicotyledonous weeds, as well as in field experiments in sunflower crops. In the greenhouse experiment treatment was carried out in phase of two true leaves by spraying plants with a herbicide separately and with the addition of SNP. The dynamics of the phytotoxic effect development was evaluated by the inhibition of the aboveground part of the plants fresh and dry matter accumulation, as well as by the inhibition of the accumulation of photosynthetic pigments in the leaves. In field experiments, the effect of SNP on the phytotoxic effect of aclonifen on cultivated plants and the effectiveness of weed control was evaluated. It was established that addition of SNP can leads to increase of aclonifen inhibitory effect on sensitive plant species at the early stages of the development of herbicide action. At the same time, the final effect of aclonifen in the range of recommended application rates of 0.6—1.2 kg/ha, both on sensitive and resistant plant species, does not change when SNP is added in concentrations of 1, 3 and 5 mM. It was concluded that join application with NO donor can leads only to the acceleration of development, but not to the strengthening of aclonifen phytotoxic effect. On the basis of a comparison of NO donor input on the phytotoxic effect of aclonifen and herbicides from the classes of synthetic auxins, PPO and ALS inhibitors, it was concluded that dependence of herbicide phytotoxic effect on the formation of ROS is a necessary, but not sufficient condition for the sensitivity of herbicide’s action to NO.
Keywords: NO, sodium nitroprusside, herbicides, aclonifen
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