Fiziol. rast. genet. 2022, vol. 54, no. 1, 40-51, doi: https://doi.org/10.15407/frg2022.01.040

Peculiarities of mesostructure and pigment complex formation in leaves of scoto- and photomorphic seedlings of horse beans under the gibberellin and tebuconazole impact

Kuryata V.G., Kuts B.O.

  • Mykhailo Kotsiubynskyi Vinnytsia State Pedagogical University 32 Ostrozhskogo St., Vinnytsia, 21000, Ukraine

Peculiarities of leaf mesostructure formation, synthesis of photosynthetic pigments under the impact of gibberellic acid and its antagonist tebuconazole in scoto- and photomorphic seedlings of horse beans were analyzed. It was established that gibberellins take an active part in the regulation of scoto- and photomorphogenesis. Gibberellic acid and tebuconazole (retardant) significantly affected the histogenesis in leaves of scoto- and photomorphic seedlings. Under the conditions of photomorphogenesis, leaves were formed thicker in comparison to seedlings that developed in the dark. At the same time, under the influence of tebuconazole the highest thickening of leaves was noted both in the dark and light. There was a decrease in leaf thickness in scotomorphic seedlings under gibberellin action. In the dark, the gibberellin effect caused the formation of thinner tissues complexes — chlorenchyma, abaxial and adaxial epidermis. The ratio between chlorophyll a and b in the control was 4.3, under the impact of tebuconazole— 4.5, and gibberellin — 3.7. Insofar as the content and ratio of chlorophylls a and b decreased under the action of gibberellin, and increased under the action of antigibberellic drug tebuconazole, this indicates the gibberellin influence on the formation of photosynthetic apparatus light-harvesting complexes. In scotomorphic seedlings, the process of conversion of unsaturated to saturated fatty acids (FA) was most inhibited by tebuconazole, and under the action of gibberellin the ratio was less. In photomorphic seedlings, this process was not inhibited either by exogenous gibberellin or by retardant, compared to control. Thus, light affects the processes of FA metabolism during the heterotrophic phase of development. Blocking the native gibberellin synthesis by tebuconazole in seedlings leads to a decrease in linolenic acid outflow from the cotyledons due to growth retardation and, consequently, the use of this fatty acid in chloroplastogenesis.

Keywords: Vicia faba L., morphogenesis, mesostructure, pigment biosynthesis, seed germination, light, gibberellins, retardants

Fiziol. rast. genet.
2022, vol. 54, no. 1, 40-51

Full text and supplemented materials

Free full text: PDF  

References

1. Golovatskaya, I.F. & Karnachuk, R.A. (2007). Dynamics of growth and the content of endogenous phytohormones during kidney bean scoto-and photomorphogenesis. Russian Journal of Plant Physiology, No. 54 (3), pp. 407-413. https://doi.org/10.1134/S102144370703017X

2. Jiang, H., Shui, Z., Xu, L., Yang, Y., Li, Y., Yuan, X., Shang, J., Asghar, M. A., Wu, X., Yu, L., Liu, C., Yang, W., Sun, X. & Du, J. (2020). Gibberellins modulate shade-induced soybean hypocotyl elongation downstream of the mutual promotion of auxin and brassinosteroids. Plant Physiology and Biochemistry, No. 150, pp. 209-221. https://doi.org/10.1016/j.plaphy.2020.02.042

3. Chen, F., Zhou, W., Yin, H., Luo, X., Chen, W., Liu, X., Wang, X., Meng, Y., Feng, L., Qin, Y., Zhang, C., Yang, F., Yong, T., Wang, X., Liu, J., Du, J., Liu, W., Yang, W. & Shu, K. (2020). Shading in mother plant during seed development promotes subsequent seed germination in soybean. Journal of experimental botany, No. 71(6), pp. 2072-2084. https://doi.org/10.1093/jxb/erz553

4. Josse, E.-M. & Halliday, K.J. (2008). Skotomorphogenesis: the dark side of light signalling. Curr. Biol., No. 18 (24), pp. 1144-1146. https://doi.org/10.1016/j.cub.2008.10.034

5. Jiang, L. & Li, S. (2015). Signaling cross talk under the control of plant photoreceptors. In: Bjorn L. (eds). (pp. 177-187). Photobiology. New York: Springer. https://doi.org/10.1007/978-1-4939-1468-5_14

6. Li, K., Gao, Z., He, H., Terzaghi, W., Fan, L. M., Deng, X. W. & Chen, H. (2015). Arabidopsis DET1 represses photomorphogenesis in part by negatively regulating DELLA protein abundance in darkness. Molecular plant, No. 8, pp. 622-630. https://doi.org/10.1016/j.molp.2014.12.017

7. Chebotar, G.O. & Chebotar, S.V. (2011). Gibberellin-signaling pathways in plants. Cytol. Genet., No. 45 (4), pp. 67-78. https://doi.org/10.3103/S0095452711040037

8. Folta, K.M., Pontin, M.A., Karlin-Neumann, G., Bottini, R. & Spalding, E.P. (2003). Genomic and physiological studies of early cryptochrome 1 action demonstrate roles for auxin and gibberellin in the control of hypocotyl growth by blue light. Plant J., No. 36 (2), pp. 203-214. https://doi.org/10.1046/j.1365-313X.2003.01870.x

9. Yamauchi, Yu., O'Neill, D. & Ogawa, M. (2004). Regulation of gibberellin metabolism by environmental factors in Arabidopsis seed germination: 45 Annual Meeting of the Japanese Society of Plant Physiologists (Tokyo, March 27-29, 2004). Plant and Cell Physiol., No. 46, p 111.

10. Wu, S.H. (2014). Gene expression regulation in photomorphogenesis from the perspective of the central dogma. Annu. Rev. Plant Biol., No. 65, pp. 311-333. https://doi.org/10.1146/annurev-arplant-050213-040337

11. Franklin, K.A. (2016). Photomorphogenesis: plants feel blue in the shade. Curr. Biol., No. 26 (24), pp. 1275-1276. https://doi.org/10.1016/j.cub.2016.10.039

12. Wang, Q. & Lin, C. (2020). Mechanisms of cryptochrome-mediated photoresponses in plants. Annu. Rev. Plant Biol., No. 71, pp. 103-129. https://doi.org/10.1146/annurev-arplant-050718-100300

13. Kutschera, U. & Briggs, W.R. (2013). Seedling development in buckwheat and the discovery of the photomorphogenic shade-avoidance response. Plant Biol. (Stuttg.), No. 15 (6), pp. 931-940. https://doi.org/10.1111/plb.12077

14. de Wit, M. & Pierik, R. (2016). Photomorphogenesis and Photoreceptors. In: Hikosaka, K., Niinemets, U., Anten, N. (eds) Canopy Photosynthesis: From Basics to Applications. Advances in Photosynthesis and Respiration (Including Bioenergy and Related Processes). Dordrecht: Springer, No. 42, pp. 171-186. https://doi.org/10.1007/978-94-017-7291-4_6

15. Kusnetsov, V.V., Doroshenko, A.S., Kudryakova, N.V. & Danilova, M.N. (2020). Role of phytohormones and light in de-etiolation. Russian Journal of Plant Physiology, No. 67, pp. 971-984. https://doi.org/10.1134/S1021443720060102

16. Wang, J., Yu, Q., Xiong, H., Wang, J., Chen, S., Yang, Z. & Dai, S. (2016). Proteomic Insight into the Response of Arabidopsis Chloroplasts to Darkness. PLoS ONE, No. 11 (5), e0154235. https://doi.org/10.1371/journal.pone.0154235

17. Zoschke, R. & Bock, R. (2018). Chloroplast translation: Structural and functional organization, operational control, and regulation. Plant Cell. No. 30, pp. 745-770. https://doi.org/10.1105/tpc.18.00016

18. Zhang, Zhao, Dongzhe, Sun, Zhang, Yue & Chen, Feng. (2020). Chloroplast morphogenesis in Chromochloris zofingiensis in the dark. Algal Research-Biomass Biofuels and Bioproducts Algal Research., No. 45, p. 101742. https://doi.org/10.1016/j.algal.2019.101742

19. Solymosi, K., Tuba, Z. & Boddi, B. (2013). Desiccoplast-etioplast-chloroplast transformation under rehydration of desiccated poikilochlorophyllous Xerophyta humilis leaves in the dark and upon subsequent illumination. Journal of Plant Physiology, No. 170 (6), pp. 583-590. https://doi.org/10.1016/j.jplph.2012.11.022

20. Masuda, T. & Fujita, Y. (2008). Regulation and evolution of chlorophyll metabolism. Photochemical & photobiological sciences: Official journal of the European Photochemistry Association and the European Society for Photobiology, No. 7 (10), pp. 1131-1149. https://doi.org/10.1039/b807210h

21. Janeckova, H., Husickova, A., Lazar, D., Ferretti, U., Pospisil, P. & Spundova, M. (2019). Exogenous application of cytokinin during dark senescence eliminates the acceleration of photosystem II impairment caused by chlorophyll b deficiency in barley. Plant Physiology and Biochemistry, No. 136, pp. 43-51. https://doi.org/10.1016/j.plaphy.2019.01.005

22. Kochubey, S.M., Bondarenko, O.Y. & Shevchenko, V.V. (2014). Photosynthesis. V. 1. The structure and functional peculiarities of light phase of photosynthesis. Kiev: Logos [in Russian].

23. Hedden, P. & Thomas, S.G. (2016). Annual Plant Review. The Gibberellins, 1st Edn. New York: Wiley Blackwell., No. 49, 472 p. https://doi.org/10.1002/9781119210436

24. Kuryata, V. & Poprotska, I. (2022). Physiological and biochemical basics of application of retardants in plant growing. Karlsruhe: ScientificWorld-NetAkhatAV. https://doi.org/10.30890/978-3-949059-42-1.2022

25. AOAC (2010). Official methods of analysis of association of analytical chemist international 18th ed. Rev. 3. Asso of Analytical Chemist. Gaithersburg, Maryland, USA.

26. Komenda, J. & Sobotka, R. (2019). Chlorophyll-binding subunits of photosystem I and II: Biosynthesis, chlorophyll incorporation and assembly. Advances in Botanical Research, No. 91, pp. 195-223. https://doi.org/10.1016/bs.abr.2019.02.001

27. de Carvalho Goncalves, J. F., Marenco, R. A. & Vieira, G. (2001). Concentration of photosynthetic pigments and chlorophyll fluorescence of mahogany and Tonka bean under two light environments. Revista Brasileira de Fisiologia Vegetal, No. 13 (2), pp. 149-157. https://doi.org/10.1590/S0103-31312001000200004

28. Boardman, N. K. (2003). Comparative Photosynthesis of Sun and Shade Plants. Annu. Rev. Plant Physiol., No. 28, pp. 355-377. https://doi.org/10.1146/annurev.pp.28.060177.002035

29. Skupien, J., Wojtowicz, J., Kowalewska, L., Mazur, R., Garstka, M., Gieczewska, K. & Mostowska, A. (2017). Dark-chilling induces substantial structural changes and modifies galactolipid and carotenoid composition during chloroplast biogenesis in cucumber (Cucumis sativus L.) cotyledons. Plant Physiology and Biochemistry, No. 111, pp. 107-118. https://doi.org/10.1016/j.plaphy.2016.11.022

30. BШddi, B., Lindsten, A. & Sundqvist, C. (1999). Chlorophylls in dark-grown epicotyl and stipula of pea. J. Photochem. Photobiol. B: Biol., No. 48, pp. 11-16. https://doi.org/10.1016/S1011-1344(99)00002-0

31. Kuryata, V., Kuts, B. & Prysedsky, Yu. (2020). Effect of gibberellin on the use of reserve substances deposited in Vicia faba L. seeds at the phase of heterotrophic development under the conditions of photo- and skotomorphogenesis. Biologija, No. 66 (3), pp. 159-167. https://doi.org/10.6001/biologija.v66i3.4311

32. Kuryata, V.G., Kuts, B.O. & Poprotska, I.V. (2021). Effect of tebuconazole on the use of reserve substances deposited in the seed of Vicia faba L. at the heterotrophic phase of development under conditions of photo- and scotomorphogenesis. Fiziol. rast. genet., 53, No. 1, pp. 63-73 [in Ukrainian]. https://doi.org/10.15407/frg2021.01.063

33. Sun, Jikang, Jia, Hao, Wang, Ping, Zhou, Tao, Wu, Yan & Liu, Zhiming. (2018). Exogenous Gibberellin Weakens Lipid Breakdown by Increasing Soluble Sugars Levels in Early Germination of Zanthoxylum Seeds. Plant Science, No. 280, pp. 155-163. https://doi.org/10.1016/j.plantsci.2018.08.013

34. Kuryata, V.G., Kuts, B.O., Poprotska I.V., Golunova, L.A., Baiurko, N.V., Nikitchenko, L.O. & Frytsiuk, V.A. (2021). Effect of gibberellin and tebuconazole on the use of seed reserve oil by Zea mays L. seedlings under photo- and scotomorphogenesis conditions. Modern Phytomorphology, No. 15, pp. 126-130. https://doi.org/10.5281/zenodo.200121

36. Tremolieres, A. & Mazliak, P. (1967). Biosynthese de d'acide a-linolenique au cours du verdissement des cotyledons etioles de trefle. Compt. Rend. Acad. Sci., No. 265 (10), pp. 1936-1945.