Fiziol. rast. genet. 2022, vol. 54, no. 6, 528-536, doi:

Sensitivity of the hair roots of Artemisia tilesii ledeb. to a complex of compounds synthesized by Priestia endophytia UCM B-7515

Matvieieva N.A., Duplij V.P., Kharkhota M.A., Brindza J., Avdieieva L.V.

  1. Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine 148 Akademika Zabolotnoho, St., Kyiv, 03143, Ukraine
  2. Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine 31/17 Vasylkivska, St., Kyiv, 03022, Ukraine
  3. D.K. Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine 154 Akademika Zabolotnogo, St., Kyiv, 03143, Ukraine
  4. Slovak University of Agricuiture in Nitra 94976 Nitra, Slovak Republic, Trieda Andreja Hlinku 2

The interaction between soil microorganisms and plants has attracted the attention of researchers for many years. This is because microorganisms in close contact with the rhizosphere can affect the vital activity of plants, thus reducing or increasing the yield of crops. At the same time, the aspect of the direct effect of compounds excreted by soil bacteria, which are known for their positive impact on plants, in particular, on their growth and biosynthetic activity, remains understudied. This work was aimed to determine the specifics of the action of the compounds contained in the sterile culture fluid (test solution) obtained after the daily cultivation of the bacteria Priestia endophytica UCM B-7515 on the growth of the «hairy» roots of the wormwood (Artemisia tilesii) as a model sample. The roots of the two lines differed in sensitivity to the test solution. An increase in the concentration of the test solution in the nutrient medium has led to the stimulation of the growth of the roots of line 10, the weight of which in all experimental variants was greater than the weight of the control roots in 1.69; 2.31; and 2.54 times when adding 0.025; 0.05; and 0.1 % of the test solution, respectively. At the same time, no differences were found in the growth of the root weight of line 4. There were no significant differences in the content of flavonoids between the control and experimental variants of line 4. The specific content of flavonoids in the control roots of line 10 was significantly higher than in the roots grown with the addition of the test solution. However, the content of flavonoids in root line 10 grown with the addition of the test solution, based on the total weight, was greater than in the control samples due to significantly faster weight increase. Therefore, a free-of-bacterial-cells culture medium obtained after daily cultivation of Priestia endophytica UCM B-7515 bacteria showed stimulating activity to the «hairy roots» of A. tilesii, although the two lines of these roots differed in their sensitivity to this solution. Thus, plant growth can be affected by even small concentrations of compounds secreted by microorganisms P. endophytica UCM B-7515 during their growth.

Keywords: «hairy roots», soil microorganisms, Priestia endophytica UCM B-7515, Artemisia tilesii, biostimulants, flavonoids

Fiziol. rast. genet.
2022, vol. 54, no. 6, 528-536

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