Fiziol. rast. genet. 2022, vol. 54, no. 5, 419-428, doi: https://doi.org/10.15407/frg2022.05.419

Increase in the phytotoxic effect of protoporphyrinogen oxidase inhibiting herbicide carfentrazone and herbicide synthetic auxin 2,4-D by join use with the NO donor sodium nitroprusside

Ponomareva I.G., Khandezhyna M.V., Radchenko M.P.

  • Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine 31/17 Vasylkivska St., Kyiv, 03022, Ukraine

The influence of the NO donor sodium nitroprusside (SNP) on the phytotoxic effect of the herbicide protoporphyrinogen oxidase (PPO) inhibitor carfentrazone and the herbicide 2,4-D from the class of synthetic auxins was studied. The study of the effect of SNP on the action of herbicides was carried out in the conditions of greenhouse experiments using oil radish (Raphanus sativus L. var. oleifera Metzg.) plants as a model of annual dicotyledonous weeds sensitive to the action of the studied herbicides. Treatment with herbicides was carried out in phase 2 of true leaves of radishes by spraying plants with solutions of herbicides and SPN separately, with solutions of herbicides with the addition of SNP, inactivated SNP and urea. The phytotoxic effect was evaluated by inhibiting the increase in the fresh mass of the above-ground part of the plants. It was established that when plants were treated with SNP solution in concentrations of 2.5 and 5.0 mM, a tendency to growth stimulation was observed. The herbicide carfentrazone at rates of 15 and 20 g/ha very weakly, and the herbicide 2,4-D at the rate of 360 g/ha significantly inhibited the growth of oil radish. An increase in the inhibitory effect of carfentrazone at a rate of 15 g/ha was observed when combined with SNP at a concentration of 5.0 mM, and at a rate of carfentrazone application of 20 g/ha at both studied concentrations of SNP 2.5 and 5.0 mM. Under join application of 2,4-D with SNP, an increase in the inhibitory effect was observed only at a SNP concentration of 5.0 mM. Addition to the solution of inactivated SNP, which lost its ability to form NO due to exposure to light for 24 hours, and urea at a concentration of 15 mM, which is equivalent to the SNP solution at a concentration of 5.0 mM in terms of nitrogen content, did not affect the herbicides action. The latter is evidence that the increase in the inhibitory effect of carfentrazone and 2,4-D by join application with SNP is due to its ability to be a NO donor. The obtained results proved the fundamental possibility of increasing the phytotoxic effect of PPO inhibitor herbicides and synthetic auxins due to the simultaneous use with the NO donor.

Keywords: NO, sodium nitroprusside, herbicides, carfentrazone. 2,4-D

Fiziol. rast. genet.
2022, vol. 54, no. 5, 419-428

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