Fiziol. rast. genet. 2022, vol. 54, no. 2, 134-147, doi: https://doi.org/10.15407/frg2022.02.134

Short-term heating causes thylakoid restructuring in pea chloroplasts and modifies spectral properties of pigment-protein complexes

Shevchenko V.V.1, Bondarenko O.Yu.1, Kornyeyev D.Yu.2

  1. Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine 31/17 Vasylkivska St., Kyiv, 03022, Ukraine
  2. Texas Tech University, Lubbock, TX 79409, USA

The effect of short-term heating on ultrastructure of the pea chloroplasts and the spectral characteristics of the subchloroplast fragments has been investigated. The size of chloroplasts decreased at heating for 5 min in the darkness at either 25, 35 or 45 °C. The phenomenon was caused mainly by a decrease of the length of long axis of chloroplast. The extent of the reduction was less after the heating at 45 °C in the presence of light. Detailed analysis has been conducted for heating at 45 °C because more prominent changes in circular dichroism (CD) spectra have been observed indicating the greater effect of heating. A decrease in y-bands of the spectrum has indicated a disturbance in long range ordering in arrangement of membrane macrodomains. The quantity of grana with an increased number of thylakoids (more than 6) became greater after the heating of isolated chloroplasts. Low temperature fluorescence spectra of chloroplasts showed a decrease in intensity of both bands of the spectrum. The spectra of subchloroplast fragments corresponding to the grana particles revealed an increase in intensity of both bands. An increase of the short-wavelength band belonging to emission of PSII, indicates an increase of quantity of PSII in grana particles originated from heated chloroplasts. An increase of the long-wavelength band belonging to PSI emission, was caused by an increasing of the size of antenna, that was detected by the rise of intensity in excitation spectrum of fluorescence detected at 735 nm. A broadening of short-wavelength band which was greater after the heating in the presence of the illumination was evoked by a loosening of the PSII complex antenna. An analysis of the spectral characteristics of the fragments of the granal thylakoids margin region provided additional data about the changes in the grana organization caused by heating. The obtained data are in line with the hypothesis that links changes in the organization of the thylakoid membranes to the interaction between PSII and the «mobile antenna» represented by light-harvesting complex II (LHCII). It has also been demonstrated that illumination mitigates the effect of short-term heating.

Keywords: Pisum sativum L., short-term heating, chloroplasts ultrastructure

Fiziol. rast. genet.
2022, vol. 54, no. 2, 134-147

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