The changes in the seeds germination capacity of the hexaploid wheat variety Odesskaya 267 after different periods of immersion in water at a temperature of 4 °C were studied. The seeds immersion in water for up to 104 hours was accompanied by rapid absorption of water in the first 32 hours (phase I), followed by a slow increase in seed moisture over the next 72 hours (phase II). The seeds sensitivity to the application of heat shock (48—52 °C) increased with the immersion time, which, together with the improvement of their germination parameters during phase I and the first part of phase II, suggests the seeds coming out of dormancy and initiating processes of germination. The sensitivity to heat shock of the seminal roots initials, especially of the radicle, gradually increased with the imbibition time expansion. Their meristems initials cells have progressed more in exit from dormancy and germination processes intensification during this period. The elaborated method of assessing the seed resistance to heat shock is prospective to compare the primary resistance (without the involvement of adaptation processes) of different wheat genotypes to high temperatures.
Keywords: Triticum aestivum L., seeds, germination, high temperature, tolerance
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1. Pachauri, R.K. & Meyer, L.A. (2014). IPCC Team: Climate change 2014: synthesis report, Contribution of Working Groups I II and III to the Fifth Assessment Report of the intergovernmental panel on Climate Change. Geneva: Switzerland.
2. Morgun, V.V., Kiriziy, D.A. & Shadchina, T.M. (2010). Ecophysiological and genetical aspects of crops adaptation to global climate changes. Physiology and biochemistry of cultivated plants, 42, No. 1, pp. 3-22 [in Russian].
3. Dascaliuc, A., Ivanova, R. & Arpentin, Gh. (2012). Systemic approach in determining the role of bioactive compounds. NATO advanced research workshop. Advanced bioactive compounds countering the effects of radiological, chemical and biological agents (pp. 9-10), Yalta [in Ukrainian]. https://doi.org/10.1007/978-94-007-6513-9_10
4. Bewleyl, J.D. (1997). Seed Germination and Dormancy. The Plant Cell, 9, No. 9, pp. 1055-1066. https://doi.org/10.1105/tpc.9.7.1055
5. Barroco, R.M., Poucke, K.V. & Bergervoet, J.H.W. (2005). The Role of the Cell Cycle Machinery in Resumption of Postembryonic Development. Plant Physiology, 137, No. 1, pp. 127-140. https://doi.org/10.1104/pp.104.049361
6. Avanzy, S., Brunory, A. & D'Aamato, F. (1969). Sequential development of meristems in the embryo of Triticum durum. A DNA autoradiographic and cytophotometric analysis. Dev. Biol., 20, No. 2, pp. 368-377. https://doi.org/10.1016/0012-1606(69)90020-7
7. Chen, D. & Osborm, D.J. (1970). Ribosomal genes and DNA replication in germinating wheat embryos. Nature, 225, No. 5230, pp. 336-340. https://doi.org/10.1038/225336a0
8. Mory, Y.Y., Chen, D. & Sarid, S. (1972). Onset of deoxyribonucleic acid synthesis in germinating wheat embryos. Plant Physiology, 49, No. 1, pp. 20-23. https://doi.org/10.1104/pp.49.1.20
9. Avanzi, S. & Deri, P.L. (1969). Duration of the mitotic cycle in two cultivars of Triticum durum, as measured by 8H-thymidine labelling. Caryologia, 22, No. 2, pp. 187-194. https://doi.org/10.1080/00087114.1969.10796337
10. Barro, R.M., Poucke, K.V. & Bergervoet, J.H.W. (2005). The role of the cell cycle machinery in resumption of postembryonic development, Plant Physiology, 137, No. 1, pp. 127-140. https://doi.org/10.1104/pp.104.049361
11. Clewer, A.G. & Scarisbrick, D.H. (2001). Practical statistics and experimental design for plant crop science. Chichester, New York: John Wiley & Sons, LTD, 332 p.
12. Bentsink, L. & Koornneef, M. (2008). Seed Dormancy and Germination. The Arabidopsis Book, American Society of Plant Biologists, No. 6, pp.2-18. https://doi.org/10.1199/tab.0119
13. Dascaliuc, A.P. (1993). Vernalization: results, problems, prospects. Chisinau, 154 p. [in Russian].
14. Fransolet, S., Deltour, R. & Bronchart, R. (1979). Changes in ultrastructure and transcription induced by elevated temperature in Zea mays embryonic root cells. Planta, 146, No. 1, pp. 7-18. https://doi.org/10.1007/BF00381249
15. Helm, K.W. & Abernethy, R.H. (1990). Heat shock proteins and their mRNAs in dry and early imbibing embryos of wheat. Plant Physiology, 93, No. 4, pp. 1626-1633. https://doi.org/10.1104/pp.93.4.1626
16. Abernethy, R.H., Thiel, D.S., Petersen, N.S. & Helm, K. (1989). Thermotolerance is developmentally dependent in germinating wheat seed. Plant Physiology, 89, No. 2, pp. 596-576. https://doi.org/10.1104/pp.89.2.569
17. Fabian, A., Jager, K. & Barnabas, B. (2008). Effects of drought and combined drought and heat stress on germination ability and seminal root growth of wheat (Triticum aestivum L) seedlings. Acta Biol Szeged., 52, No. 1, pp. 157-159.