The aim of recearch was to investigate the effect of different use of exogenous lectin in combination with the treatment of seeds with fungicides Fever and Standak Top on symbiotic systems of soybean—Bradyrhizobium japonicum. It has been found that the treatment of seeds with fungicides Fever and Standak Top leads to inhibition of symbiotic soybean systems functioning in the phase of three true leaves. However, at later development stages (flowering and beans formation) these drugs have a stimulating effect on the activity of legume-rhizobial systems. It has been shown that exogenous lectin as a component of bacterial suspension, or at seeds treatment, had a stimulating effect on the symbiotic and photosynthetic apparatus of soybean, with the maximum effect in the case of seeds treatment with lectin. At the same time, the nature and extent of the effect that this protein cause on symbiotic soybean systems when applied together with fungicides depended on the phase of plant development and the type of fungicide.
Keywords: Bradyrhizobium japonicum, symbiosis, nitrogen-fixing activity, net assimilation rate, exogenous lectin, fungicides
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1. Koliada, V. (2011.) Sources of stabilization and increase of soybean yield in Ukraine. Ahronom, 1, pp. 144-149 [in Ukrainian].
2. Petrenkova, V.P. & Sokol, T.V. (2013). Soybean diseases. Posibnyk Ukrainskoho khliboroba, 2, pp. 29-31 [in Ukrainian].
3. Sheludko, O., Klubuk, V., Stavratii, V., Repilevskyi, E., Markovska, O. & Salhalov, O. (2014). Application of fungicides on irrigated soybean crops. Propozytsiia, 1, pp. 90-92 [in Ukrainian].
4. Kots, S.Ya., Morhun, V.V., Patyka, V. F., Malychenko, S.M., Mamenko, P.N., Kiriziy, D.A., Mykhalkyv, L.M., Berehovenko, S.K. & Melnykova, N. N. (2011). Biological fixation of nitrogen: legume-rhizobial symbiosis. [Monograph: in 4 tons.]. Kyiv: Lohos [in Russian].
5. Alieksieiev, O.O., Patyka, V.P. & Hnatiuk, T.T. (2016). The relationship between Bradyrhizobium japonicum and soybean bakteriosis pathogens and their sensitivity to pesticides. Molodyi vchenyi. Silskohospodarski nauky, 40, No. 12, pp. 60-63 [in Ukrainian].
6. Volkohon, V.V., Dimova, S.B., Volkohon, K.I., Borulko, R.O. & Berdnykov, O.M. (2010). Influence of microbial preparations on the absorption of nutrients by cultivated plants. Visnyk ahrarnoi nauky: Zemlerobstvo, hruntoznavstvo, ahrokhimiia, 5, pp. 25-28 [in Ukrainian].
7. Belaieva, V.N., Paniuta, O.O. & Taran, N.Yu. (2009). The role of lectins in plant protective reactions to phytopathogens. Fyzyolohyia i byokhymyia kult. rasteniy, 41, No. 3, pp. 221-234 [in Ukrainian].
8. Melnykova, N.M., Mykhalkiv, L.M., Mamenko, P.M. & Kots, S.Ya. (2013). The areas of application for plant lectins. Biopolymers and Cell, 29(5). pp. 357-366 [in Ukrainian]. https://doi.org/10.7124/bc.00082A
9. Lubianova, A.R., Bezrukova, M.V., Fatkhutdynova, R.A. & Shakyrova, F.M. (2009). The growth-promoting and protective effects of phytohemagglutinin on bean plants. Visnyk Kharkivskoho natsionalnoho ahrarnoho un-tu. Seriia biolohiia, 2, No. 17, pp. 40-46 [in Russian].
10 Babosha, A.V. (2008). Inducible lectins and plant resistance to pathogens and abiotic stress. Biokhimiia, 73, No. 7, pp. 1007-1022 [in Russian]. https://doi.org/10.1134/S0006297908070109
11. Chen, J., Liu, B., Ji, N., Zhou, J., Bian, H.J., Li, C.Y., Chen, F. & Bao, J.K. (2009). A novel sialic acid-specific lectin from Phaseolus coccineus seeds with potent antineoplastic and antifungal activities. Phytomedicine, 16, pp. 352-360. https://doi.org/10.1016/j.phymed.2008.07.003
12. Wang, X., Bauw, G., Van Damme, E.J., Peumans, W.J., Chen, Z.L., Van Montagu, M., Angenon, G. & Dillen, W. (2001). Gastrodianin-like mannose-binding proteins: a novel class of plant proteins with antifungal properties. The Plant journal for cell and molecular biology, 25, pp. 651-661. https://doi.org/10.1046/j.1365-313x.2001.00999.x
13. Saha, P., Dasgupta, I. & Das, S. (2006). A novel approach for developing resistance in rice against phloem limited viruses by antagonizing the phloem feeding hemipteran vectors. Plant molecular biology, 62, pp. 735-752. http:/doi.org/10.1007/s11103-006-9054-6 https://doi.org/10.1007/s11103-006-9054-6
14. Shakyrova, F.M., Khairullyn, R.M. & Yamaleev, A.M. (1990). Comparative analysis of lectin and abscisic acid content in wheat seedlings infected with root rot. Immunofermentnyiy analiz regulyatorov rosta rasteniy. Primenenie v fiziologii rasteniy i ekologii. Ufa: BNTs UrO AN SSSR. pp. 38-41.
15. Child, J.J. (1975). Nitrogen fixation by a Rhizobium sp. association with nonleguminous plant cell cultures. Nature, 253, pp. 350-351. https://doi.org/10.1038/253350a0
16. Hardy, R.W.F., Holsten, R.D., Jackson, E.K. & Burns, R.C. (1968). The acetylene-ethylene assay for N2 fixation: laboratory and field evaluation. Plant Physiology, 43, pp. 1855-1207. https://doi.org/10.1104/pp.43.8.1185
17. Mokronosov, A.T. & Kovalev, A.H. (Eds.). (1989). Photosynthesis and bioproductivity: methods of determination. Moscov: Ahropromyzdat [in Russian].
18. Dospekhov, B.A. (1985). Methodology of field experiment. Moscov: Ahropromyzdat [in Russian].
19. Veselovska, L.I. (2015). The role of lectin in the adaptation of legume-rhizobial soybean symbiosis to drought. Avtoreferat dys. … kand. byol. nauk. Kyiv, 21 p. [in Ukrainian].
20. Tykhonovych, Y.A. (2005). Integration of plant genetic systems and microorganisms in symbiosis. Uspekhy sovremennoi byolohii, 125, No. 3, pp. 227-238 [in Russian].
21. Morgun, V.V., Schwartau, V.V. & Kiriziy, D.A. (2009). Physiological bases of formation of high productivity of cereals. Fiziolohiia roslyn: problemy ta perspektyvy rozvytku. Kyiv: Lohos, Vol. 1 [in Ukrainian].