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Fiziol. rast. genet. 2019, vol. 51, no. 4, 315-323, doi: https://doi.org/10.15407/frg2019.04.315

Cell selection with heavy metal ions for obtaining salt tolerant plant cell cultures

Sergeeva L.E., Mykhalska S.I.

  • Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine 31/17 Vasylkivska St., Kyiv, 03022, Ukraine

The cell selection with heavy metal ions was proposed for obtaining salt tolerant plant cell lines. On selective media with the addition of Ba2+ cations resistant soybean and wheat cell variants were obtained. Doses of Ba2+ cations were lethal for wild type cell cultures. The selected clones were tested under stress pressure of various agents: Ba2+ ions, sea water salts, sodium sulfate. Resistant cells sustained any type of lethal stress. Calli retained salt tolerance after cultivation on salt-free medium during several passages. During cultivation under salinity cells demonstrated low K+/Na+ ratios and high free proline accumulation. The Ba2+ provoked opposite cell reactions. It is assumed that combined stress resistance is concerned with realization of different protection mechanisms.

Keywords: soybean, wheat, cell selection, Ba2+ cations, salt tolerance

Fiziol. rast. genet.
2019, vol. 51, no. 4, 315-323

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