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Fiziol. rast. genet. 2019, vol. 51, no. 4, 308-314, doi: https://doi.org/10.15407/frg2019.04.308

Features of the dynamics of flavonoid accumulation in Artemisia tilesii Ledeb.

Duplij V.P., Matvieieva N.A.

  • Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine 148 Academika Zabolotnoho St., 03143, Kyiv, Ukraine

Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine 148 Academika Zabolotnoho St., 03143, Kyiv, Ukraineе-mail: duplijv@icbge.org.ua

Flavonoids are compounds with high biological activity, which are used in pharmacology. They are synthesized in plants of different species. However, the synthesis of flavonoids depends on the conditions of cultivation. “Hairy” root culture obtained by Agrobacterium rhizogenes-mediated genetic transformation can be an alternative source of flavonoids. Determination of flavonoids content in various samples of “hairy” roots of Artemisia tilesii Ledeb. and dynamics of their accumulation was the aim of the work. The roots were grown under sterile conditions at 24 °C. For determining the flavonoid content samples were collected after 20, 30 and 40 days of the cultivation. Flavonoids were quantified by a standard method using of aluminum chloride solution. Significant differences were founded in the total content of flavonoids in six lines of “hairy” roots of A. tilesii, which varied from 5,45±0,41 to 14,79±3,16 mg/g of fresh weight after 40 days of cultivation. The differences in the dynamics of accumulation of the investigated compounds in the samples were also studied. In particular, the relative increase of flavonoids content in the sample No. 45 was the highest at the last stage of cultivation (between the 30th and 40th days). At the same time this coefficient in the sample No. 44 significantly increased between 20th and 30th day of growing. Such differences may be the result of nondetermined place of foreign genes (in particular rol genes) incorporation after Agrobacterium-mediated transformation. This results indicate the possibility to obtain A. tilesii “hairy” roots characterized by high level of flavonoids and a rapid increase in their content.

Keywords: Artemisia tilesii Ledeb., flavonoids, genetic transformation, “hairy” roots

Fiziol. rast. genet.
2019, vol. 51, no. 4, 308-314

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