Fiziol. rast. genet. 2018, vol. 50, no. 2, 149-160, doi:


Krutylo D.V., Nadkernychna O.V.

  • Institute of Agricultural Microbiology and Agro-industrial Manufacture, National Academy of Agrarian Sciences of Ukraine 97 Shevchenko St., Chernihiv, 14027, Ukraine

The peculiarities of soybean and cowpea plants interaction with slow and intensively growing strains of Bradyrhizobium japonicum of different genetic groups have been studied. Soybean plants are able to enter into effective symbiotic relationships with all the investigated rhizobia strains independently of their growth rate and genetic affiliation. Cowpea plants are selective according to soybean nodule bacteria strains. Interacting with intensively growing USDA 123 В. japonicum strains they form ineffective symbiosis with low level of N2 fixation. Otherwise they form full value N2-fixing systems with slow growing strains of USDA 4, USDA 6 and USDA 110 genetic groups. Rhizobia with different growth rate have identical RFLP-profiles of nifH genes. The formation of active (Nod+Fix+ phenotype) and inactive (Nod+Fix— phenotype) symbiotic systems of cowpea with rhizobia is not associated with the structure of mentioned gene. Likely it can be determined by partners’ specificity of genotype interaction on early stages of symbiosis development.

Keywords: Bradyrhizobium japonicum, soybean, cowpea, symbiotic system, specificity, RFLP-analysis, nifH gene

Fiziol. rast. genet.
2018, vol. 50, no. 2, 149-160

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