Fiziol. rast. genet. 2017, vol. 49, no. 4, 300-311, doi: https://doi.org/10.15407/frg2017.04.300

CYTOGENETIC ACTIVITY OF RADIONUCLIDE CONTAMINATED SUPERFICIAL WATER RESERVOIRS IN THE AFFECT ZONE OF TAILING STORAGE OF URANIUM MINING INDUSTRY

Yakimchuk R.A.

  • Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine 31/17 Vasylkivska St., Kyiv, 03022, Ukraine

A cytogenetic analysis of root meristem cells of Triticum aestivum L., whose seeds were grown in the conditions of natural radionuclide contaminated water and bottom deposits of water reservoirs in the affect zone of tailing storage of uranium mining industry, was made. Frequency of chromosome aberrations exceeds a spontaneous level by 1.3—4.4 times. Type spectrum of cytogenetic disorders includes acentric fragments and bridges. Increased frequency of the cells with multiple aberrations in the conditions of effect of water reservoirs radionuclides confirms their high mutagenic activity, which can be partially explained by specific response of the restoration system to radiation-induced cytogenetic disorders in the range of low-rate radiation.

Keywords: Triticum aestivum L., cytogenetic disorders, chromosome aberrations, mitosis anomaly, radionuclide contamination, superficial water reservoirs

Fiziol. rast. genet.
2017, vol. 49, no. 4, 300-311

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References

1. Artamonov, V.M. & Kamuz, A.M. (2007). Influence of activity of CJSC "Vesco" on the state of contamination of the Kazenii Torets River basin by surface-active substances. Problemy ekolohii, No. 1-2, pp. 16-20 [in Ukrainian].

2. Baranov, V., Bania, A., Bodnar, L., Blaida, I. & Karpenko, O. (2014). Toxicological analysis of water of drainage channels and ash dust from Dobrotvirskaya TPP. Visn. Lviv. un-tu. Ser. Biolohiia, No. 65, pp. 238-244 [in Ukrainian].

3. Bolsunovskiy, A.Ya., Muratova, E.N., Sukovatyiy, A.G., Pimenov, A.V., Sanzharaeva, E.A., Zotina, T.A., Sedelnikova, T.S., Pankov, E.V. & Kornilova, M.G. (2007). Radioecological monitoring of the Yenisei River and cytogenetic characteristics of the aquatic plant Elodea canadensis. Radiation biology. Radioecology, 47, No. 1, pp. 63-73.

4. Voitsekhovych, O.V. (2008). Performance of work in accordance with the programs and regulations of radiation monitoring: Report on the results. Gospotogovir 9 from the State Enterprise "Barrier" of 28.05.2008. Kyiv: TsMDPT [in Ukrainian].

5. Grodzinskiy, D.M. & Gudkov, I.N. (2006). Radiation damage to plants in the zone of influence of the Chernobyl accident. Radiation biology. Radioecology, 46, No. 2, pp. 189-199.

6. Gudkov, D.I., Kaglyan, A.E., Klenus, V.G., Shirokaya, Z.O. & Ganzha, K.D. (2015). Current levels and dynamics of radionuclide contamination of components of aquatic ecosystems in the Chernobyl Exclusion Zone. Nauk. zap. Ternop. nats. ped. un-tu. Ser. Biolohiia, 64, No. 3-4, pp. 149-152 [in Ukrainian].

7. Gudkov, D.I., Kuzmenko, M.I., Kireev, S.I. & Nazarov, A.B. (2009). Radioecological problems of aquatic ecosystems in the Chernobyl Exclusion Zone. Radiation biology. Radioecology, 49, No. 2, pp. 192-202.

8. Hudkov, D., Kuzmenko, M., Kirieiev, S. & Nazarov O. (2008). Radioecological problems of aquatic ecosystems of the Chornobyl NPP exclusion zone. Visn. NAN Ukrainy, No. 4, pp. 44-55 [in Ukrainian].

9. Dolina, L.F., Gunko, E.Yu. & Mashihina, P.B. (2016). Protection of water from radioactive contamination. Dnipropetrovsk: Lira [in Russian]. https://doi.org/10.15802/978-966-383-685-0

10. Dospehov, B.A. (1985). Methods of field experience (with the basics of statistical processing of research results). Moscow: Kolos [in Russian].

11. Zaychenko, E.Yu., Severinovskaya, E.V. & Dvoretskiy, A.I. (2014). Environmental hazard of radiation and chemical pollution in the Prydniprovsky region. Ekolohiia i pryrodokorystuvannia, No. 18, pp. 84-94 [in Russian].

12. Zanizdra, V.S. & Zhdanova, G.V. (2013). Monitoring studies of radioactivity in the uranium production waste repository "Dneprovskoye". Zb. nauk. prats Sevastop. nats. un-tu yadernoi enerhii ta promyslovosti, No. 2, pp. 101-110 [in Russian].

13. Kyrychenko, V.K. (2015). Radioecological danger and additional dose burden on the population from the tailings of the Prydniprovsky chemical plant (Unpublished candidate thesis). National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine [in Ukrainian].

14. Kovaleva O.A. (2008). Cytogenetic abnormalities in mammalian somatic cells. Cytology and genetics, 42, No. 1, pp. 58-72.

15. Kovalevskiy, L.I., Operchuk, A.P. & Los, I.P. (2008). The state of radiation safety in the uranium mines of Ukraine. Dovkillia ta zdorovia, No. 2, pp. 4-8 [in Russian].

16. Kuzmenko, M.I. (2013). Radionuclide anomaly. Kyiv: Akademperiodyka [in Ukrainian].

17. Kutsokon, N.K., Bezrukov, V.F., Lazarenko, L.M., Rashydov, N.M. & Grodzynskyi, D.M. (2003). Number of aberrations on aberrant cell as a parameter of chromosomal instability. Cytology and genetics, 37, No. 4, pp. 20-25.

18. International IAEA RW management programs (2012). Retrieved from http://ecsocman.hse.ru

19. From myths and phobias to the revival of production safety at the site of the former PO Pridneprovsky Chemical Plant (based on the results of an expert meeting at the SE Barrier 11.08.2016) (2016). Retrieved from http://baryer.dp.ua/ index.php/uk/press-office/news-and-events/53-ot-mifov-i-fobij-k-vozrozhdeniyu-bezopasnosti-proizvodstv-na-ploshchadke-byvshego-po-pridneprovskij-khimicheskij-zavod

20. Pausheva, Z.P. (1988). Workshop on plant cytology. Moscow: Agropromizdat [in Russian].

21. Pismennaya, O.B. (2009, October). Economic evaluation of the desirability of desalting the mine waters of uranium mines. Proceedings of the 5th International Scientific Conference Problems of natural resources use of sustainable development and technogenic safety of regions (pp. 74-75), Dnipropetrovsk.

22. Rozhko, M.M., Ersteniuk, H.M. & Kryzhanivska, A.Ie. (2014). Development and introduction of a system for reducing the man-caused load on the territory and population of ecologically crisis areas. Ekolohiia i pryrodokorystuvannia, No. 18, pp. 97-110 [in Ukrainian].

23. Romanenko, V.D. (2006). Methods of hydroecological surveys of surface waters. Kyiv: Lohos [in Ukrainian].

24. Rudenko, L.I., Han, V.E., Odintsov, A.A. & Dzhuzha, O.V. (2013). Phase distribution, forms of location and the proportion of microparticles in the groundwater of 137Cs, 90Sr, uranium and transuranium elements. Dop. NAN Ukrainy, No. 7, pp. 165-171 [in Russian].

25. Riabchenko, N.M. (2014, April). Cytogenetic indices in the assessment of individual radiation sensitivity of a person. Proceedings of the International Scientific Conference Radioecology 2014 (pp. 236-240), Zhytomyr [in Ukrainian].

26. Serdyuk, S. M. (2007). Diagnostics of heavy metal pollution of the soil cover of industrial-urbanized areas. Ekolohiia ta noosferolohiia, 18, No. 3-4, pp. 133-138 [in Russian].

27. Skliar, N. (2009). Hostage tailings. Emergency situation, No. 6, pp. 24-26 [in Ukrainian].

28. Soroka, Yu.M., Rets, Yu.M. & Chesanov, V.L. (2016). Justification of radiation safety when designing measures to clear the Konoplyanka river. Stroitelstvo, materialovedenie, mashinostroenie. Ser. Bezopasnost zhiznedeyatelnosti, No. 93, pp. 106-112 [in Ukrainian].

29. Sumatokhina, I.M., Duk, N.M. & Shevchenko, O.A. (2008). Industrial waste as a factor in the state of ecological safety of the region: assessment, mapping, management. Ekolohiia dovkillia ta bezpeka zhyttiediialnosti, No. 1, pp. 69-75 [in Ukrainian].

30. Timofeev-Resovskiy, N.V. & Ivanov, V.I. (1968). Application of the principle of hit in radiobiology. Moscow: Atomizdat [in Russian].

31. Tyapkin, O.K., Podrezenko, I.N., Ostapenko, N.S. & Kryuchkova, S.V. (2016). Features of monitoring studies of technogenic influence on hydraulic systems in mining regions. Gornyiy inform.-analit. byul., No. 3, pp. 235-340 [in Russian].

32. Shkarupa, V.M., Neumerzhytska, L.V., Klymenko, S.V. & Symihlazova, T.V. (2011). Dynamics of changes in spectrum of aberrations of chromosomes induced by mitomycin C in Allium cepa L. Visn. UTHiS, 9, No. 1, pp. 112-117 [in Ukrainian].

33. Shmakova, N.L., Nasonova, E.A. & Krasavin, E.A. (2006). Induction of chromosomal aberrations and micronuclei in human peripheral blood lymphocytes under the action of low doses of radiation. Radiation biology. Radioecology, 46, No. 4, pp. 480487.

34. Yakymchuk, R.A. & Valiuk, V.F. (2017, May). The mutagenic activity of radionuclide contaminations of the reservoirs of the near-exclusion zone of the Chernobyl Nuclear Power Plant. Proceedings of the 1st International Scientific Conference Problems of Ecology and Evolution of Ecosystems in Conditions of the Transformed Environment (pp. 176-181) [in Ukrainian].

35. DSTU ISO 5667-6–2001 (2002). Water quality. Sampling Part 6. Guidelines for the sampling of water from rivers and other watercourses. Kyiv [in Ukrainian].

36. DSTU ISO 5667-4–2003 (2003). Water quality. Sampling Part 4. Guidance on the sampling of natural and artificial lakes. Kyiv [in Ukrainian].

37. Dvoretsky, A.I., Belokon, A.S. & Severinovskaya, E.V. (2001). Ecological-toxicological estimation of superficial water quality of middle Dnieper in conditions of anthropogenic influence. Efficient Water Management: World Water Congress.

38. Geras'kin, S.A., Oudalova, A.A. & Kim, J.K. (2007). Cytogenetic effect of low dose g-radiation in Hordeum vulgare seedlings: non linear dose-effect relationship. Radiat. Environ. Biophys, 46, No. 1, pp. 31-41. https://doi.org/10.1007/s00411-006-0082-z

39. Naito, K., Kusabba, M. & Shikazono N. (2005). Transmissible and nontransmissible mutations induced by irradiation Arabidopsis thaliana pollen with g-rays and carbon ions. Genetics, No. 169, pp. 881-889. https://doi.org/10.1534/genetics.104.033654

40. Puchta, H. (2005). The repair of double-strand breaks in plants: molecular mechanisms and consequences for genome evolution. J. Exp. Bot., 56, No. 409, pp. 1-14.

41. Takatsuji, T., Takayanagi, H. & Morishita, K. (2010). Induction of micronuclei in germinating onion seed root tip cells irradiated with high energy heavy ions. J. Rad. Res., 51, No. 3, pp. 315-323. https://doi.org/10.3103/S0095452713010118