One of the main characteristics that determines quality of wheat is grain endosperm texture. 90 lines of generation F5, obtained by crossing a hexaploid spring donor, carrying the substituted wheat chromosome 6B from Triticum turgidum spp. dicoccoides, with bread winter wheat cultivar Kuialnyk, were analyzed for the presence of gene Gpc-B1 from T. turgidum spp. dicoccoides. Selected 44 lines with homozygous gene Gpc-B1 were checked for composition of alleles of genes Pina-D1, Pinb-D1. The analysis revealed a wide variety of combinations of the alleles. 16 lines were analyzed by the near infrared spectroscopy (NIR) method. It was determined mainly all they belong to a group of soft wheat varieties according to the starch composition. The statistical analysis shows a direct relationship between core grain hardness and allelic composition of puroindoline genes.
Keywords: grain hardness of wheat, gene Gpc-B1, genes Pina-D1, Pinb-D1, molecular marker, biofortification
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