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Fiziol. rast. genet. 2017, vol. 49, no. 2, 129-133, doi: https://doi.org/10.15407/frg2017.02.129

Nitrate reductase activity in biotechnology tobacco plants under enzyme inhibitors action

Sergeeva L.E.

  • Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine 31/17 Vasylkivska St., Kyiv, 03022, Ukraine

Nitrate reduction process occurs in plant only with the participation of enzyme nitrate reductase (NR). Tobacco cell lines resistant to lethal doses of the tungsten (WO42-) and vanadium (VO3-) anions, which inhibit NR, were obtained via cell selection. R0 plants were regenerated from some lines. The NR activity was estimated in leaves. Under normal conditions the NR activity in regenerants exceeded this parameter in wild type plants at 2—3 times. Under enzyme inhibitory pressure conditions NR activity in regenerants was maintained at considerable level too. Probable causes of obtained effects are discussed.

Keywords: tobacco, cell selection, tungstate, vanadate, nitrate reductase

Fiziol. rast. genet.
2017, vol. 49, no. 2, 129-133

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