The dynamics of ABA content and activity in the leaves and shoot apical meristems (SAM) of isogenic by E genes soybean lines in the conditions of different photoperiod have been studied in field experiments. ABA content and activity in the leaves and SAM of all soybean isolines increased irrespective of photoperiod duration. However, the magnitude of this process depended on E gene alleles of isoline genotype as well as on photoperiod duration. The PIS (photoperiodic insensitive) lines e1E2e3 and e1e2e3 showed the higher ABA accumulation in SAM both under long and short day. The SD (short-day) lines E1E2E3, E1e2e3 and PIS line e1e2E3 showed the higher content and activity of ABA in the leaves under long day. On the contrary, in short day ABA level was higher in the SAM of these isolines. It is supposed that E genes probably affect the transition to flowering of soybean under different photoperiod through their participation in the regulation of activity, accumulation and balance of ABA in the leaves and shoot apical meristems.
Keywords: Glycine max (L.) Merr., soybean, isogenic lines, E genes, photoperiod, shoot apical meristems, leaves, ABA
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1. Polevoi, V.V. & Chirkova, T.V. (Eds.). (2001). Workshop on plant growth and resistance. S.-Pb.: Izd-vo S.-Peterburgskogo un-ta [in Russian].
2. Savinskii, S.V., Dragovoz, I.V. & Pedchenko, V.K. (1991). Determination of the content of zeatin, indolyl-3-acetic and abscisic acids in one plant sample by high performance liquid chromatography. Fiziologia i biokhimia kult. rastenij, 23, No. 6, pp. 611-618 [in Russian].
3. Yukhno, Yu.Yu. & Zhmurko, V.V. (2010). The rates of development and the growth processes of soybean isogenic lines under different day-length conditions. Visnyk Kharkivskogo natsionalnogo universitetu imeni V.N Karazina. Seriya: biologiya, Iss.11, No. 905, pp. 210-223 [in Ukrainian].
4. Achard, P., Cheng, H., De Grauwe, L., Decat, J., Schoutteten, H. & Moritz, T. (2006). Integration of plant responses to environmentally activated phytohormonal signals. Science, 31, No. 5757, pp.91-94. https://doi.org/10.1126/science.1118642
5. Blazquez, M.A., Trenor, M. & Weigel, D. (2002). Independent control of gibberellin biosynthesis and flowering time by the circadian clock in Arabidopsis. Plant Physiol., 130, pp. 1770-1775. https://doi.org/10.1104/pp.007625
6. Boss, P.K., Bastow, R.M., Mylne, J.S. & Dean, C. (2004). Multiple pathways in the decisions to flower: Enabling, promoting, and resetting. Plant Cell, 16, pp. 18-31. https://doi.org/10.1105/tpc.015958
7. Davis, SJ. (2009). Integrating hormones into the floral-transition pathway of Arabidopsis thaliana. Plant Cell and Environment, 32, pp. 1201-1210. https://doi.org/10.1111/j.1365-3040.2009.01968.x
8. Domagalska, M.A., Sarnowska, E., Nagy, F. & Davis, S.J. (2010). Genetic analyses of interactions among gibberellin, abscisic acid, and brassinosteroids in the control of flowering time in Arabidopsis thaliana. PLoS One, 5. Retrieved from https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0014012. https://doi.org/10.1371/journal.pone.0014012
9. El-Antably, H.M.M., Wareing, P.F. & Hillman, J. (1967). Some physiological responses to d,1 abscisin (dormin). Planta, 73 (1), pp. 74-90. https://doi.org/10.1007/BF00419842
10. Fornara, F., Montaigu, A. & Coupland, G. (2010). SnapShot: Control of flowering in Arabidopsis. Cell, 141, pp. 550-550. https://doi.org/10.1016/j.cell.2010.04.024
11. Gomez, L.D., Baud, S., Gilday, A., Li, Y. & Graham, I.A. (2006). Delayed embryo development in the ARABIDOPSIS TREHALOSE-6-PHOSPHATE SYNTHASE 1 mutant is associated with altered cell wall structure, decreased cell division and starch accumulation. Plant J., 46, pp. 69-84. https://doi.org/10.1111/j.1365-313X.2010.04312.x
12. Jackson, St.D. (2009). Plant responses to photoperiod. New Phytologist, 181, pp. 517-531. https://doi.org/10.1111/j.1469-8137.2008.02681.x
13. Jack, T. (2004). Molecular and genetic mechanisms of floral control. Plant Cell, 16, pp. 1-17. https://doi.org/10.1105/tpc.017038
14. Jung, Ch.-H., Wong, Ch.E., Singh, M.B. & Bhalla, P.L. (2012). Comparative genomic analysis of soybean flowering genes. PLoS One, 7 (6). Retrieved from https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0038250. https://doi.org/10.1371/journal.pone.0038250
15. King, R.W. & Evans, L.T. (2003). Gibberellins and flowering of grasses and cereals: Prizing open the lid of the «Florigen» black box. Plant Biol., 54, pp. 307-328. https://doi.org/10.1146/annurev.arplant.54.031902.135029
16. Martinez-Zapater, J.M., Coupland, G., Dean, C. & Koorneef, M. (2994). The transition to flowering in Arabidopsis. Cold Spring Harbor: Cold Spring Harbor Lab. Press.
17. Podolnyi, V.Z., Josefusova, Z., Khmelnitskaya, I.Ph., Verenchikov, S.P., Krekule, J. & Chailakhyan, M.Kh. (1989). Abscisic acid as a potent regulator of the transition from juvenile to mature stage in Xanthium strumarium. Biol. Plant., 31, pp. 139-144. https://doi.org/10.1007/BF02907247
18. Price, W.B. (2012). Understanding the mechanisms of the photoperiod flowering pathway in soybean. Master's thesis. Urbana, University of Illinois at Urbana-Champaign. Retrieved from https://www.ideals.illinois.edu/bitstream/handle/ 2142/34233/Price_William.pdf.
19. Quecini, V., Zucchi, M.I., Baldin, J. & Vello, N.A. (2007). Identification of soybean genes involved in circadian clock mechanism and photoperiodic control of flowering time by in silico analyses. J. Integr. Plant Biol., 49, pp. 1640-1653. https://doi.org/10.1111/j.1774-7909.2007.00567.x
20. Razem, F.A., El-Kereamy, A., Abrams, S.R. & Hill, R.D. (2006). The RNA-binding protein FCA is an abscisic acid receptor. Nature, 439, pp. 290-294. https://doi.org/10.1038/nature04373
21. Simpson, G.G. & Dean, C. (2002). Arabidopsis, the rosetta stone of flowering time? Science, 296, pp. 285-289. https://doi.org/10.1126/science.296.5566.285
22. Tasma, I.M. & Shoemaker, R.C. (2003). Mapping flowering time gene homologs in soybean and their association with maturity (E) loci. Crop Sci., 43, pp. 319-328. https://doi.org/10.2135/cropsci2003.0319
23. Thakare, D., Kumudini, S. & Dinkins, RD. (2010). Expression of flowering-time genes in soybean E1 near-isogenic lines under short and long day conditions. Planta, 231, pp. 951-963. https://doi.org/10.1007/s00425-010-1100-6
24. Vanneste, S. & Friml, J. (2009). Auxin: A Trigger for change in plant development. Cell, 136, pp. 1005-1016. https://doi.org/10.1016/j.cell.2009.03.001
25. Wang, W., Chen, W., Chen, W., Hung, L. & Chang, P. (2002). Influence of abscisic acid on flowering in Phalaenopsis hybrida. Plant Physiol. Bioch., 40, pp. 97-100. https://doi.org/10.1016/S0981-9428(01)01339-0
26. Wang, Y., Wu, C-X., Zhang, X-M., Wang, Y.-P. & Han, T.-F. (2008). Effects of soybean major maturity genes under different photoperiods. Acta Agronomica Sinica, 34, No.7, pp. 1160-1168. https://doi.org/10.3724/SP.J.1006
27. Wilmowicz, E., Kesy, J. & Kopcewicz, J. (2008) Ethylene and ABA interactions in the regulation of flower induction in Pharbitis nil. J. Plant Physiol., 165, pp. 1917-1928. https://doi.org/10.1016/j.jplph.2008.04.009
28. Wong, Ch.E., Singh, M.B. & Bhalla, P.L. (2009). Floral initiation process at the soybean shoot apical meristem may involve multiple hormonal pathways. Plant Signal Behav., 4, pp. 648-651. https://doi.org/10.4161/psb.4.7.8978
29. Wong, Ch.E., Singh, M.B. & Bhalla, P.L. (2009). Molecular processes underlying the floral transition in the soybean shoot apical meristem. Plant J., 57, pp. 832-845. https://doi.org/10.1111/j.1365-313X.2008.03730.x
30. Wong, Ch.E., Singh, M.B. & Bhalla, P.L. (2013). The dynamics of soybean leaf and shoot apical meristem transcriptome undergoing floral initiation process. PLoS ONE, 8, e65319. https://doi.org/10.1371/journal.pone.0065319