Fiziol. rast. genet. 2021, vol. 53, no. 5, 425-434, doi:

Anatomic-physiological changes in horse bean seedlings under the influence of gibberellin and tebuconazole at conditions of photo- and scotomorphogenesis

Kuryata V.G., Kuts B.О., Poprotska I.V.

  • Vinnytsia Mykhailo Kotsiubynskyi State Pedagogical University 32 Ostrozkogo St., Vinnytsia 21000, Ukraine

The combination of light/dark external factor, GA3 and GA3 synthesis inhibitor tebuconazole significantly changed the pattern of the source-sink relationship in sprouts of horse bean during seed germination. The usage of gibberellin at light leads to increase of reserve starch hydrolysis in germinating horse bean seeds. The absence of light was a more significant factor for the starch hydrolysis than the exogenous use of gibberellin and the antigibberellin substance tebuconazole. The nitrogen-containing compounds content and reserve fats at early stages of germination in cotyledons did not change significantly, indicating less intensive use of these compounds for morphogenesis during this period. Seed germination was accompanied by a decrease under the action of gibberellin at light, and under the action of tebuconazole — an increase, in root and epicotyl diameter due to the peculiarities of histogenesis. Under the influence of gibberellic acid, total epiblem and the primary root cortex thickened at light, and the epidermis and the primary cortex of hypocotyl — at light and dark. The opposite change was caused by tebuconazole — the increase of tissue complex thickness occurred under both photo- and scotomorphogenesis conditions. The number of xylem vessels in the vascular-fibrous bunches in roots increased under the influence of tebuconazole in dark. In epicotyls, the increase occurred in the number of vessels in bunches under the action of tebuconazole both at light and in dark. Insofar as histogenesis is controlled by phytohormones, established histological changes indicated a significant re-structuring of the entire hormonal complex of seedlings.

Keywords: Vicia faba L., morphogenesis, source-sink system, seed germination, light, gibberellins, retardants

Fiziol. rast. genet.
2021, vol. 53, no. 5, 425-434

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