Fiziol. rast. genet. 2021, vol. 53, no. 5, 415-424, doi:

Transient expression of uida and gfp genes in Physalis peruviana

Yaroshko O.M., Matvieieva N.A., Kuchuk M.V.

  • Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine 148 Akademika Zabolotnoho St., Kyiv, 03143, Ukraine

Physalis peruviana is a plant species which finds its application in agriculture and food industry due to the synthesis of numerous compounds such as fisagulins, fisalingulinides, visangulatine, fisalins. In recent years the properties of Physalis were improved with genetic engineering methods. We studied the possibility of transient expression of genes in P. peruviana plants using the Agrobacterium rhizogenes and A. tumefaciens. The intact plants were cultivated in greenhouse (22—26 °С, 14-hour light photoperiod, 3000—4500 lx) before transformation. A. rhizogenes A4 carrying pICH5290 or pCB131 vector and A. tumefaciens with pICBV19 vector were used for obtaining plants with transiently expressed reporter uidA and gfp genes. Also we additionally used A. tumefaciens GV3101 strain with pICH6692 genetic vector with gene coding the P19 protein (the suppressor of gene silencing). The pICH5290 genetic vector contained bar and gfp genes; the pCB131 genetic vector contained the bar, gfp and fbpB (DTMD) — gene, coding Ag85B of Mycobacterium tuberculosis. The pCBV19 genetic vector contained uidA and bar genes. Transient expression of uidA and gfp genes was confirmed in adult plants after conduction of vacuum infiltration with the bacteria. Maximum of gfp expression was observed between the 5th and 12th days after the transformation. The most intensive expression of the gfp and uidA genes was detected in the upper leaves (2nd—3rd) of young plants. There was no significant difference in gene expression levels in case of using agrobacteria with or without the genetic vector which carried out the gene silencing suppressor.

Keywords: Physalis peruviana L., Agrobacterium, transformation, transient expression, uidA, gfp gene

Fiziol. rast. genet.
2021, vol. 53, no. 5, 415-424

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