Results on the studies of kernels and their structural parts: endosperm, pericarp and the germ of the maize hybrid ZP 633 that is very desirable for human consumption are presented. For the first time, the method of infrared spectroscopy was apply to record and study all spectral bands (of very high, high, low and very low intensity) of the infrared spectrum of maize hybrid kernels, endosperm, pericarp and the germ. Based on performed studies it can be stated that infrared spectra of maize hybrid kernels, endosperm, pericarp and the germ are characterised by all their spectral bands and several parameters: number, intensity, kinetics and the distribution of the site of origin in the wavenumber range of 400—4000 cm–1. These parameters were particularly observed for both kernels and their parts (endosperm, pericap and the germ). Spectral bands of very high and high intensity are also characterised by the stated parameters: the number that usually ranges from 3 to 5, intensity, various kinetic forms, as well as the distribution of their origin within the wavenumber range. These spectral bands make possible the identification of organic compounds, their fragments and molecular structures that determine them. In case of spectral bands for the kernel, endosperm, pericarp and the germ with very high and high intensity, organic compounds can be identified as: proteins, lipids, sugars, esters, amides, ketones, aldehydes, carboxylic acids, ethers, phenols, alcohols, aromatic carbohydrates, acyclic compounds, alkenes, alkanes, and alkynes. Spectral bands of the kernel, endosperm, pericarp and the germ of low and very low intensity are also characterised by the stated parameters: the number that can differ, low intensity, the distribution of sites of their origin, and especially the frequency of vibrations of valence bonds of functional groups of organic molecules. Spectral bands of the kernel, endosperm, pericarp and the germ of low and very low intensity, also provide the possibility to identify the excited state of molecular structures and valence bonds of functional groups of organic compounds. The excited state of molecular structures and valence bonds of functional groups of organic compounds is expressee in different forms of vibration movements: symmetric and asymmetric valence vibration (stretching), deformation vibration of valence bonds and molecular structures (scissoring), rocking of valence bonds and molecular structures (rocking), wagging of valence bonds and molecular structures (wagging), twisting of valence bonds and molecular structures (twisting), trembling of valence bonds and molecular structures (trembling).
Keywords: Zea mays L., hybrid, kernel, pericarp, endosperm, germ, infrared spectrum, spectral band, organic molecule, functional group, excited state, vibration of chemical bonds
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1. Vasiliev, A.V., Grinenko, E.V., Schukin, A.O. & Fedulina, T.G. (2007). Infrared spectroscopy of organic and natural compounds. St. Petersburg: St. Petersburg. Gos. Forestry technician. Acad. [in Russian].
2. Tarasevich, B.N. (2012). IR spectra of the main classes of organic compounds. Moscow: Publishing. Moscow State University [in Russian].
3. Radenoviє, ‡.N., Maksimov, G.V. & Grodzinskij, D.M. (2015). Identification of Organic Molecules in Kernels of Maize Inbred Lines Displayed with Infrared Spectra. Fisiol. rast. genet., 47, No. 1, pp. 15-24.
4. Radenoviє, ‡.N., Maksimov, G.V., Tyutyaev, E.V., Syusin, I.V., Shutova, V.V., Secanski, M.D., Srdiє, J.¦., Videnoviє, ¦.V. &Popoviє, A.S. (2015). Structural Properties of Maize Hybrids Established by Infrared Spectra, Maticasrpska J. Nat. Sci., No. 129, pp. 35-44. https://doi.org/10.2298/ZMSPN1529035R
5. Radenovich, C.H., Maksimov, G.V., Tutyaev, E.V., Shutova, V.V., Delich, N., Chamdzhia, Z., Pavlov, J. & Jovanovic, J. (2016). Identification of organic compounds in corn hybrids (Zea mays L.) of Serbian breeding using infrared spectra. Selskokhozyaystvennayabiologiya, 51, No. 5, pp. 645-653 [in Russian]. https://doi.org/10.15389/agrobiology.2016.5.645eng
6. Salam, A.Aboud, Ammar, B.Altemimi, Asaad, R.S.Al-HiIphy, Lee,Yi-Chen & Francesco, Cacciola 3. (2019). A Comprehensive Review on Infrared Heating Applications in Food Processing. Molecules, 24, 4125. https://doi.org/10.3390/molecules24224125
7. Radenoviє, ‡.N., Deliє, N.S., Radosavljeviє, M.M., Jovanoviє, ¦.V., Se№anski, M.D., Popoviє, A.S., Crevar, M.S. & Radosavljeviє, N.D. (2021). High-yielding and chemically enriched maize hybrids bred in Serbia - the best basis for super quality feed and food. Military technical courier, Vol. 69, No. 1. https://doi.org/10.5937/vojtehg69-29512
8. Radosavljeviє, M. & Radenoviє, ‡. (2020). Laboratory procedures and operating techniques for the extraction of endosperms, pericarps and germs from kernels of maize hybrids. Internal publication of the Maize Research Institute, Zemun Polje, Belgrade, pp. 1-3.
9. Radosavljeviє, M., Bekriє, V., Boыoviє, I. & Jakovljeviє, J. (2000). Physical and chemical properties of various corn genotyps as a criterion of technological qality. Genetika, 32(3), pp.319-329 [online]. Available at: http://www.dgsgenetika.org.rs/abstrakti/vol. 32_2000_no3_en.htm#Rad9 [Accessed: 15 November 2020].
10. Amir, R.M., Anjum, F.M., Khan, M.I., Khan, M.R., Pasha, I. & Nadeem, M. (2013). Application of Fourier transform infrared (FTIR) spectroscopy for the identificationof wheat. J. Food Sci. Technol., 50, pp. 1018-1023. https://doi.org/10.1007/s13197-011-0424-y
11. Jackson, M. & Mantsch, H.H. (2006). Infrared spectroscopy, ex vivo tissue analysis. In: Biomedical Spectroscopy. Encyclopedia of Analytical Chemistry (pp. 131-156), JohnWiley & Sons Ltd. https://doi.org/10.1002/9780470027318.a0107
12. Vollhardt, P.C. & Schore, N.E. (1996). Organic Chemistry. New York: W.H. Freeman and Company.
13. White, P.J. & Johnson, L.A. (2003). Corn: Chemistry and Technology. Minnesota: American Association of Cereal Chemists
14. Skoog, D.A., Holler, F.J. & Crouch, S.R. (2007). Principles of instrumental analysis. Belmont: Thomson Higher Education.
15. Kols, O.R., Maksimov, G.V. & Radenovich, Ch.N. (1993). Biophysics of Rhythmic Excitation. Moscow: Publishing. Moscow State University [in Russian].
16. Radenoviє, ‡. (1998). Transport Processes Across the Membrane. In: Contemporary Biophysics. Velarta, pp. 1-90, Belgrade.
17. Macura, S. & Radenoviє, ‡. (2016). In order to acquire better knowledge on a biological system, besides the genome and proteome it is necessary to know its metabolome, i.e. concentrations of all metabolites and their interactions, Written Correspondence, Mayo Clinic, Rochester & Maize Research Institute, Zemun Polje, Belgrade and vice versa.
18. Radenoviє, ‡.N., Maksimov, G.V., Shutova, V.V., Deliє, N.S., Milenkoviє, M.V., Pavloviє, M.D. & Beljanski, M.V. (2018). The study by the methods of infrared spectroscopy of the stretching and twisting vibrations of chemical bonds in functional groups of organic compounds contained in grains of maize inbred lines. Fisiol. rast. genet., 50, No. 4, pp. 322-330. https://doi.org/10.15407/frg2018.04.322
19. Radenoviє, ‡.N., Maksimov, G.V., Shutova, V.V., Slatinskaya, O.V., Protopopov, F.F., Deliє, N.S., Grchiє, N.M., Pavlov, J.M. & ‡amdыija, Z.F. (2019). Complete study of nature and importance of spectral bands contained in infrared spectra of leaves of maize inbred lines with significant breeding properties. Russian Agricultural Sciences, 2019, 45, No. 4, pp. 334-339. https://doi.org/10.3103/S106836741904013X