Plazmid vector pSUP5011::Tn5mob was used for transposon mutagenesis in Rhizobium galegae strains 159, 0702, 0703. The frequency of Kmr-mutants formation after transfer this vector from Escherichia coli to Rhizobium galegae was (1,3—1,9) · 10-7. As a result of screening Tn5-mutants of R. galegae on the characteristics of «virulence», «nitrogen fixation», «efficiency of symbiosis» genetically marked rhizobia with improved properties were selected. Obtained data indicate the possibility of creating highly efficient symbiotic systems Galega orientalis L.—Tn5-mutants R. galegae.
Keywords: Rhizobium galegae, Galega orientalis L., symbiosis, nitrogen fixation, nodule bacteria, transposone mutagenesis
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