The selection of callus lines of winter triticale resistant to simulated salt stress by the direct and step-type in vitro selection with application of selective system based on sodium chloride were carried out. There were identified 5 and 4 resistant callus lines from line 38/1296 and variety Obriy respectively, that had a high survival rate on the selective medium with 1.2 % NaCl and maintained morphogenetic potential. Plant regenerants were induced from the resistant lines and their rearing, rooting and transfer to in vivo conditions were optimized. Generation R1 was obtained from the induced regenerants and its resistance to simulated salt stress was analyzed.
Keywords: Triticosecale, in vitro selection, salt stress, callus cultures, plant regenerants, resistance
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