Phosphinothricin resistant wheat plants of native breeding cv. Zymoyarka carrying bar gene of bacterium Streptomyces hygroscopicus have been obtained by two methods of genetic transformation. The biolistic transformation was performed with vector pAHC25 while the Agrobacterium-mediated one with pCB203 in strain GV3101. Both vectors in addition to the selective gene (bar) of phosphinothricin acetyl transferase contained the reporter gene (uidA) of b-glucuronidase from Escherichia coli. The immature embryos were used as primary explants. The integration of transgene bar into regenerants’ genome and the lack of agrobacterium infection were proved by means of PCR. The expression of gene uidA was confirmed by histochemical analysis. The transformation efficiency amounting to 0.5 % for biolistic and to 1.25 % for Agrobacterium-mediated allowed us to select 3 and 12 transgenic lines, respectively. This is the first report on the successful establishment of transgenic wheat resistant to the herbicide phosphinothricin in in vitro culture in Ukraine.
Keywords: Triticum aestivum L., bread wheat, genetic transformation of plants, plant biotechnology, herbicides
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