Modifications of structural properties and protein content of the membranes from the distict regions of thylakoid system in chloroplasts after 5 minutes of warming in the darkness have been investigated. Analysis of the fractions obtained after fragmentation of chloroplasts with digitonin followed by differential centrifugation revealed differences in the yield of fractions and the content of major protein complexes. It was demonstrated that short-term warming in the darkness at 25 °C «loosened» the structure of grana system. Heat treatment at 45 °C led to compacting of stacking zone of granal thylakoids. However, the stacking region was estimated to be smaller because of higher yield of particles originated from peripheral regions of granal thylakoids.
Keywords: Pisum sativum L., chloroplasts, photosynthetic membranes, thylakoid system, grana, protein complexes
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