Fiziol. rast. genet. 2016, vol. 48, no. 6, 538-543, doi: https://doi.org/10.15407/frg2016.06.538

The fertility and viability of pollen grains of Iris hybrida hort. plant varieties introduced into the Forest-Steppe of Ukraine

Skrypka G.I.

  • >M.M. Grishko National Botanic Garden, National Academy of Sciences of Ukraine 1 Tymiryazevs’ka St., Kyiv, 01014, Ukraine

A study of pollen grains of Iris hybrida hort. plants was conducted in the Forest-Steppe growth conditions of Ukraine. Pollen fertility and viability of 8 varieties were analyzed. Indices of fertility varied from 91,1 (Tashkent) to 98,7 % (River Hawk). The best artificial growth medium for pollen germination was established to be 10—15 % sucrose solution with 2,5 % agar. In a 10 % sucrose solution the viability of male gametes varied from 59,1 (River Hawk) to 84,7 % (Latin Rock), in a 15 % solution — from 42,0 (Before The Storm) to 90,0 % (Latin Rock).

Keywords: Iris hybrida hort., variety, fertility, viability, pollen grains, Forest-Steppe of Ukraine

Fiziol. rast. genet.
2016, vol. 48, no. 6, 538-543

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References

1. Alekseeva, T.G. (2012). Determination of the viability of pollen and embryo sac. Methodological instructions for a large special practicum. Odesa: Odesa National University Publishing [in Ukrainian].

2. Golubinskii, I.N. (1974). Biology of pollen germination. Kyiv: Naukova Dumka [in Russian].

3. Dolganova, Z.B. (2003). Biological aspects of increasing the productivity and decorative character of rhizospheric perennials in the forest-steppe zone of Western Siberia. (Unpublished doctoral thesis). M.A. Lisavenko Institute of Horticulture of Siberia, Barnaul, Russia. [in Russian].

4. Zaytsev, G.N. (1973). Methodology of biometric calculations. Moscow: Nauka [in Russian].

5. Kirpicheva, L.F. Palinologic studies of garden bearded irises in the conditions of the foothills of the Crimea. Proceedings of the 2nd International Scientific Conference Biodiversity and sustainable development (pp.86-89). Simferopol, Ukraine [in Russian].

6. Kirpicheva, L.F. (2008). Rhythms of growth and development of hybrid iris (Iris hybrida hort.) in the conditions of the foothills of the Crimea. Visnyk of Bilotserkivskii National Agrarian University., No. 54, pp. 86-89 [in Russian].

7. Nekrasov, V.I. (1982). Comparative characteristics of the quality of pollen and seeds of some herbaceous introducents. Botanical Garden Bulletin, No. 123, p. 31 [in Russian].

8. Pausheva, Z.P. (1988). Plant cytology workshop. Moscow: Agropromizdat (in Russian).

9. Rodionenko, G.I. (1970). The phenomenon of temporary loss of pollen activity. Bot. journal, No. 2, pp. 300-302 [in Russian].

10. Skrypka, H.I. (2014, September). Quality of pollen of Iris hybrida hort. in the conditions of the forest-steppe of Ukraine. Advances in botany and ecology, proceedings of the young scientist conference (p. 143), Uman [in Ukrainian].

11. Tarasenko, R.O. (2008). Informational technologies. Kyiv: Alefa [in Ukrainian].

12. Stanley, R.G. & Linskens, H.F. (1974). Pollen: Biology, Biochemistry, Management. Berlin: Springer-Verlag. https://doi.org/10.1007/978-3-642-65905-8

13. Takhtajan, A. (2009). Flowering Plants 2nd ed. Springer Science + Business Media B.V. https://doi.org/10.1007/978-1-4020-9609-9