For the first time the HPLC method was used to identify cytokinins and to determine their content in micelial biomass of six mushroom species (Ganoderma lucidum, Trametes versicolor, Fomitopsis officinalis, Pleurotus nebrodensis, Grifola frondosa, Sparassis crispa). Basidiomycetes were studied for cytokinins production for the purpose to prepare medicines from fungal raw material with a high cytokinin activity for therapeutic effects and to produce medicinal — prophylactic food additives and pure drugs. Trans- and cis-zeatin, zeatin riboside, zeatin-O-glucoside, isopentenyladenosine, isopentenyladenine were found, but only one species (G. lucidum) contained all these forms. The cytokinins highest total content was revealed in F. officinalis. The T. versicolor and G. lucidum micelial biomass showed the highest production of riboside-type cytokinins (zeatin riboside and isopentenyladenosine), which possess therapeutic properties (antiproliferative and cytotoxic effects on cancer cells). F. officinalis, T. versicolor and G. lucidum are regarded as perspective basidiomycetes for the biotechnological development to obtain drugs with a high biological activity from micelial biomass.
Keywords: basidiomycetes, medicinal mushrooms, cytokinins, phytohormones
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