Fiziol. rast. genet. 2016, vol. 48, no. 6, 498-507, doi:

Genetic stability of micropropagated plants of Deschampsia antarctica Desv. during long-term in vitro culture

Spiridonova K.V.1, Andreev I.O.1, Zagrichuk O.M.2, Navrotska D.O.1, Twardovska M.O.1, Drobyk N.M.2, Kunakh V.A.1

  1. Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine 150 Akad. Zabolotnogo St., Kyiv, 03680, Ukraine
  2. Volodymyr Hnatyuk Ternopil National Pedagogical University 2 M. Kryvonosa St., Ternopil, 46027, Ukraine

In order to establish the suitability of previously developed micropropagation protocol to get genetically uniform plants of D. antarctica the genetic variation of the plants in the process of propagation and maintenance in vitro was examined using PCR analysis and cytogenetic analysis. The clonal progeny was evaluated for the plants that differed by the molecular-genetic markers and cytogenetic characteristics. Compаrative analysis of plants at the initial stages (1—6 passages) of propagation and after a long-term maintenance in vitro (24—26 passages and more) does not reveal genetic differentiation among the clones of common origin and their original genotype by ISSR-markers. Analysis of long-term cultured plants (49—79 passages) also showed maintenance of initial cytogenetic characteristics in the micropropagated plants. In general, the results prove that the developed technique provides the preservation of genetic chаrаcteristics of D. antarctica during long-term maintenance in vitro and can be used to produce genetically uniform plant material.

Keywords: Deschampsia antarctica, micropropagation, genome variation, PCR-analysis, cytogenetic analysis

Fiziol. rast. genet.
2016, vol. 48, no. 6, 498-507

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