Fiziol. rast. genet. 2016, vol. 48, no. 3, 215-231, doi: https://doi.org/10.15407/frg2016.03.215

The study of biological nitrogen fixation at the Institute of Plant Physiology and Genetics of NAS of Ukraine

Kots S.Ya.

  • Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine 31/17 Vasylkivska St., Kyiv, 03022, Ukraine<

The most important results of the study of biological fixation of atmospheric nitrogen conducted at the Department of Symbiotic Nitrogen Fixation of the Institute of Plant Physiology and Genetics of NAS of Ukraine from 1976 to 2016 are reported. Much attention is paid to the results of the study obtained during the last twenty years.

Keywords: nitrogen fixation, symbiosis, assosiations, strains of microorganisms, nitrogenase

Fiziol. rast. genet.
2016, vol. 48, no. 3, 215-231

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References

1. Belyma, N.Y. & Manoryk, A.V. (1967). The content of free amino acids in the molasses, roots and root secretions of some plants. Fiziologo-biohimicheskie osnovy pitaniya rastenij. Kyiv: Nauk. Dumka, Vol. 2, pp. 63-71 [in Ukrainian].

2. Vasyliuk, V.M., Krugova, O.D., Mandrovska, N.M. & Kots, S.Y. (2007). Peroxidase and catalase activities in the soybean inoculated by Tn5-mutants of Bradyrhizobium japonicum. Fiziologia i biokhimia kult. rastenij, 39, No. 4, pp. 334-342 [in Ukrainian].

3. Vasyliuk, V.M., Melnykova, N.M., Mykhalkiv, L.M., Omelchuk, S.V. & Kots, S.Ya. (2007). Formation of symbiotic interactions between lupine plants and transposon mutants of Bradyrhizobium sp. (Lupinus). Fiziologia i biokhimia kult. rastenij, 39, No. 3, pp. 233-241 [in Ukrainian].

4. Vasyliuk, V.M. (2008). Physiological peculiarities of investigations soybean and lupine with new nodule bacteria (Bradyrhizobium) strains, obtained by transposone mutagenesis (Extended abstract of candidate thesis) Institute of Plant Physiology and Genetics National Academy of Sciences of Ukraine, Kyiv, Ukraine [in Ukrainian].

5. Veselovska, L.I., Mykhalkiv, L.M. & Kots, S.Ya. (2013). The influence of exogenous lectin on the effectivity of Glycine max-Bradyrhizobium japonicum symbiosis under drought conditions. Fiziol. rast. genet., 45, No. 4, pp. 319-326 [in Ukrainian].

6. Veselovska, L.I. (2015). The role of lectin in the adaptation of the legume-rhizobium symbiosis of soybean to drought (Extended abstract of candidate thesis) Institute of Plant Physiology and Genetics National Academy of Sciences of Ukraine, Kyiv, Ukraine [in Ukrainian].

7. Volkogon, M.V., Mamenko, P.M. & Kots, S.Ya. (2009). IAA and zeatin balance in soybean plants under seeds inoculation with various strains and mutants of Bradyrhizobium japonicum. Fiziologia i biokhimia kult. rastenij, 41, No. 5, pp. 408-416 [in Ukrainian].

8. Vorobey, N.A., Kots, S.Ya. & Butnitskiy, I.M. (2007). Effectiveness of alfalfa symbiotic systems at inoculation with Tn5-mutants Sinorhizobium meliloti. Fiziologia i biokhimia kult. rastenij, 39, No. 2, pp. 105-113 [in Ukrainian].

9. Gryshchuk, O.O. & Kots, S.Ya (2013). Ability of strains and Tn5-mutants of Bradyrhizobium japonicum to synthesize zeatin and gibberellins at conditions in vitro. Fiziologia i biokhimia kult. rastenij, 45, No. 2, pp. 148-154.

10. Gryshchuk, O.O. (2014). Phytohormonal status of soybean plants at use of strains with different symbiotic characteristics. (Extended abstract of candidate thesis) Institute of Plant Physiology and Genetics National Academy of Sciences of Ukraine, Kyiv, Ukraine [in Ukrainian].

11. Drozdenko, G.M., Mamenko, P.M. & Kots, S.Ya. (2013). The activity of guaiacol- and ascorbate peroxidase and protein composition of soybean roots during the formation and the early functioning symbiotic system of Glycine max-Bradyrhizobium japonicum. Bull. Kharkiv Nat. Agrar. Univ. Ser. Biol., Vol. 1, pp. 18-26 [in Ukrainian].

12. Drozdenko, G.M., Mamenko, P.M., Malichenko, S.M. & Kots, S.Ya. (2009). Characteristics of protein composition of strains and tn5-mutants of Bradyrhizobium japonicum of various activity. Fiziologia i biokhimia kult. rastenij, 41, No. 5, pp. 423-429 [in Ukrainian].

13. Zhemojda, A.V., Omelchuk, S.V., Malichenko, S.M. & Kots, S.Ya. (2013, March). Investigation of toxic effects of fungicides of Lamardor and Maxim on the growth and development of Bradyrhizobium japonicum in pure culture. Materials of the scientific and methodical seminar Plant physiology in the system of modern biological knowledge and sciences (pp. 74-76), Kharkiv [in Ukrainian].

14. Musiyaka, L.L. & Polyakova, L.L. (1984). Institute of Plant Physiology NAS of USSR. Kyiv: Nauk. Dumka [in Ukrainian].

15. Kyrychenko, O. (2014). Crop Biotechnology. Nikolaev: Ilion [in Russian].

16. Kyrychenko, O.V. (2002). Relationships of leguminous plants and nodule bacteria on the level of before-contact interactions when forming nitrogen-fixing systems. Fiziologia i biokhimia kult. rastenij, 34, No. 2, pp. 95-101 [in Russian].

17. Kyrychenko, O.V. (2005). Study of chemotaxis of nodule bacteria of lupine to organic substances. Mikrobiolohichnyi Zhurnal, 67, No. 3, pp. 19-26 [in Russian].

18. Kirichenko, E.V., Malichenko, S.M. & Starchenkov, E.P. (1995). Lectin of leguminous plants as a molecular component of carbohydrate protein system of symbionts recognition. Fiziologia i biokhimia kult. rastenij, 27, No. 5-6, pp. 315-323 [in Russian].

19. Kyrychenko, O.V., Titova, L.V., Zhemojda, A.V. & Omelchuk, S.V. Effect of leguminous plant lectin of different specifity on the development of crop seedlings. Fiziologia i biokhimia kult. rastenij, 36, No. 5, pp. 390-397 [in Russian].

20. Kyrychenko, O.V. (2011). Biological activity of exogenous lectins at forming and functioning of phytobacterial associations. Bull. Kharkiv Nat. Agrar. Univ. Ser. Biol., 2 (23), pp. 46-59.

21. Kyrychenko, O.V. & Zhemojda, A.V. (2006). Ecological aspects of application of vegetative lectins for the presowing treatment of seeds of soya bean and wheat. Agroecological journal, No. 3, pp. 42-48 [in Ukrainian].

22. Kyrychenko, O.V., Malichenko, S.M. & Kosenko, L.V. (2001). Exopolysaccharides of lupin and soybean nodule bacteria and their role in the formation of symbiotic interconnections between rhizobia and leguminous plants. Fiziologia i biokhimia kult. rastenij, 33, No. 6, pp. 539-544 [in Ukrainian].

23. Kyrychenko, O.V. & Perkovskaya G.Yu. (2005). Effect of endogenous wheat lectin on flavonoid content and change in lectin activity of wheat seedlings at UV-radiation. Biopolymers and Cell, 21, No. 5, pp. 413-418 [in Ukrainian]. https://doi.org/10.7124/bc.000704

24. Kyrychenko, O.V. & Sergienko, V.G. (2006). Fungitoxic activity of plant lectins. Fiziologia i biokhimia kult. rastenij, 38, No. 6, pp. 526-534 [in Ukrainian].

25. Kyrychenko, O.V. (2005). Chemotaxis of Rhizobium of lupin to the amino acids, saccharides, solts of organic acids at the presence of mineral nitrogen. Fiziologia i biokhimia kult. rastenij, 37, No. 4, pp. 333-340 [in Ukrainian].

26. Kiriziy, D.A., Veselovska, L.I. & Kots, S.Ya. (2014). The influence of drought on gas exchange of leaves of soybean inoculated by rhizobia under seed lectin application. Fiziol. rast. genet., 46, No. 6, pp. 498-506 [in Ukrainian].

27. Kondratiuk, Iu., Mamenko, P. & Kots, S. (2014). The influence of different doses of mineral nitrigen on total protein content of roots and nodules of soybean, inoculated by strains of Bradyrhizobium japonicum with contrasting symbiotic features. Visnyk Kyivskoho natsionalnoho universytetu imeni Tarasa Shevchenka. Seriia Biolohiia, 1, No. 66, pp. 37-41[in Ukrainian].

28. Kondratiuk, Iu.Iu., Mamenko, P.M. & Zhemojda, A.V. (2014). Protein compositions of soybean roots inoculated by Bradyhizobium japonicum strains with various activity under optimal and insufficient water supply. The Scientific Issues of Ternopil Volodymyr Hnatiuk National Pedagogical University. Series Biology, 60, No. 3, pp. 106-109 [in Ukrainian].

29. Kondratiuk, Iu.Iu., Mamenko, P.M., Levishko, A.S., Drozdenko, G.M. & Kots, S.Ya. (2013). Comparative analysis of methods for protein extraction and separation for proteomic investigation of soybean roots and nodules proteins. Fiziologia i biokhimia kult. rastenij, 45, No. 3, pp. 222-229 [in Ukrainian].

30. Kondratiuk, Iu.Iu., Rybachenko, O.R., Mamenko, P.M. & Kots, S.Ya. (2015). The influence of drought on the protein content of soybean roots under inoculation by Bradyrhizobium japonicum strains with different effectiveness. Fiziol. rast. genet., 47, No. 4, pp. 361-366 [in Ukrainian].

31. Kosenko, L.V., Mykhalkiv, L.M., Krugova, E.D., Mandrovskaya, N.M. & Kots, S.Ya. (2003). Biological activity of glucan Sinorhizobium meliloti. Microbiology, No. 5, pp. 633-638 [in Ukrainian].

32. Kots, S.Ya., Beregovenko, S.K., Kyrychenko, E.V. & Melnykova, N.N. (2007). Features of the interaction of plants and nitrogen-fixing microorganisms. Kyiv: Nauk. Dumka [in Russian].

33. Kots, S.Ya., Veselovska, L.I. & Mykhalkiv, L.M. (2014). The nitrate reductase activity in the leaves of soybean inoculated with Bradyrhizobium japonicum under different water supply and lectin application. The Scientific Issues of Ternopil Volodymyr Hnatiuk National Pedagogical University. Series Biology, No. 3 (60), pp. 114-117 [in Ukrainian].

34. Kots, S.Ya., Volkogon, N.V. & Gryshchuk, O.O. (2010). Ability of strains and tn5-mutants of Bradyrhizobium japonicum to synthesize iaa and aba in vitro Fiziologia i biokhimia kult. rastenij, 42, No. 6, pp. 491-496 [in Ukrainian].

35. Kots, S.Ya. (1997). Influence of various doses of mineral nitrogen and strains of Rhizobium meliloti on the synthesis of free amino acids in the overweight alfalfa. Fiziologia i biokhimia kult. rastenij, 29, No. 4, pp. 310-316 [in Ukrainian].

36. Kots, S.Ya., Grigoryuk, I.A., Mykhalkiv, L.M., Beregovenko, S.K. & Dragovoz, I.V. (2006). Effect of natural and synthetic plant growth regulators on the nitrogen-fixing and photosynthetic activities of alfalfa under different water supply conditions. Agricultural Chemistry, No. 5, pp. 41-48 [in Russian].

37. Kots, S.Ya., Kiriziy, D.A. & Veselovska, L.I. (2013). The intensity of photosynthesis of the soybean  Bradyrhizobium japonicum symbiotic systems under exogenous lectin influence. Reports of the National Academy of Sciences of Ukraine, No. 11, pp. 157-162 [in Ukrainian].

38. Kots, S.Ya., Malichenko, S.M., Krugova, O.D., Mandrovska, N.M. & Kyrychenko, O.V. (2001). Physiological and biochemical features of plant nutrition by biological nitrogen. Kyiv: Logos [in Ukrainian].

39. Kots, S.Ya., Mykhalkiv, L.M., Veselovska, L.I. & Beregovenko, S.K. (2014). The nitrogen fixing activity and productivity of alfalfa inoculated with Sinorhizobium meliloti under different water supply and using of protein with hemagglutinin activity. Bull. Kharkiv Nat. Agrar. Univ. Ser. Biol., 23, No. 1129, pp. 59-64 [in Ukrainian].

40. Kots, S.Ya. & Mykhalkiv, L.M. (2005). Physiology of symbiosis and nitrogen nutrition of alfalfa. Kyiv: Logos [in Ukrainian].

41. Kots, S.Ya., Morgun, V.V., Patyka, V.P., Datsenko, V.K., Krugova, O.D., Kyrychenko, O.V., Melnykova, N.M. & Mykhalkiv, L.M. (2010). Biological nitrogen fixation: legume-rhizobial symbiosis. Vol. 1. Kyiv: Logos [in Russian].

42. Kots, S.Ya., Morgun, V.V., Patyka, V.P., Malichenko, S.M., Mamenko, P.M., Kirizii, D.A., Mykhalkiv, L.M., Beregovenko, S.K. & Melnikova, N.M. (2011). Biological nitrogen fixation: legume-rhizobial symbiosis. Vol. 2. Kyiv: Logos [in Russian].

43. Kots, S.Ya., Morgun, V.V., Patyka, V.P., Petrychenko, V.F., Nadkernichnaya, E.V. & Kyrychenko, O.V. (2014). Biological nitrogen fixation: associative nitrogen fixation. Vol. 4. Kyiv: Logos [in Russian].

44. Kots, S.Ya., Morgun, V.V., Tikhonovich, I.A., Provorov, N.A., Patyka, V.P., Petrychenko, V.F., Melnykova, N.M. & Mamenko, P.M. (2011). Biological nitrogen fixation: genetics of nitrogen fixation, genetic engineering of strains. Vol. 3. Kyiv: Logos [in Russian].

45. Krugova, E.D., Kots, S.Ya., Mandrovskaya, N.M. & Vasyliuk, V.N. (2008). Peroxidase and polyphenol oxidase activities in soybean nodules and roots, inoculated by Tn5-mutants of Bradyrhizobium japonicum. Bull. Kharkiv Nat. Agrar. Univ. Ser. Biol., 1, pp. 6-14 [in Russian].

46. Krugova, E.D. (2009). Specific strategies of nodule and phytopathogenic bacteria at infection of plants. Fiziologia i biokhimia kult. rastenij, 41, No. 1, pp. 3-15 [in Russian].

47. Krugova, O.D. (2009). Peroxidase and polyphenol oxidase activities in symbiotic soybean systems formed with the participation of Bradyrhizobium japonicum mutants in the early stages of the formation of symbiosis. In Morgun, V.V. (Ed.) Plant physiology: problems and perspectives of development (pp. 428-436), Kyiv: Logos [in Ukrainian].

48. Krugova, O.D., Mandrovska, N.M. & Kyrychenko, O.V. (2006). Effect of exogenous lectin on the endogenous lectin and antioxidant enzymes activity and flavonoide content in wheat. The Ukrainian Biochemical Journal, 78, No. 2, pp. 106-112 [in Ukrainian].

49. Levishko, A.S, Mamenko, P.M. & Kots, S.Ya. (2014). Dynamics of organic acids in soybean roots inoculated by Bradyrhizobium japonicum strains with different symbiotic properties. The Scientific Issues of Ternopil Volodymyr Hnatiuk National Pedagogical University. Series Biology Biolohichna fiksatsiia azotu, pp. 118-121 [in Ukrainian].

50. Levishko, A.S, Mamenko, P.M. & Kots, S.Ya. (2013, March). Research of distribution of metabolites of bean-rhizobial symbiosis of different efficiency. Materials of the scientific and methodical seminar Plant physiology in the system of modern biological knowledge and sciences (pp. 84-85), Kharkiv [in Ukrainian].

51. Levishko, A.S & Mamenko, P.M. (2014, November). Metabolic changes in soybean roots inoculated by Bradyrhizobium japonicum strains with different symbiotic properties. Materials of the III International sciences conf. Regulation of growth and development of plants: physiological and biochemical and genetic aspects (pp. 93-94), Kharkiv.

52. Levishko, A.S & Mamenko, P.M. (2014, April). Peculiarities of functioning of soybean-rhizobial symbiosis of different efficiency. Materials of the international sciences conf. Microbiology and immunology - prospects for development in the twenty-first century (pp. 67-68), Kyiv [in Ukrainian].

53. Levishko, A.S & Khomenko, Yu.O. (2012). Dynamics of metabolites distribution in the functioning of soybean-rhizobial symbiosis of different efficacy. Scientific notes of the ecological laboratory USPU, 15, pp. 57-61 [in Ukrainian].

54. Malichenko, S.M., Datsenko, V.K., Vasyliuk, V.M. & Kots, S.Ya. Transposonium mutagenesis of strains of Bradyrhizobium japonicum. Fiziologia i biokhimia kult. rastenij, 39, No. 5, pp. 409-418 [in Ukrainian].

55. Malichenko, S.M., Datsenko, V.K., & Mamenko ,P.M. (2001, October). Adsorption of nodule bacteria by roots of specific and non-specific legume plants. Materials of the International sciences conf. Plant ontogenesis, biological fixation of molecular nitrogen and nitrogen metabolism (pp. 95-98), Ternopil [in Ukrainian].

56. Malichenko, S.M., Datsenko, V.K., Mamenko, P.M. & Kots, S.Ya. (2002). Participation of lectins specific and non-specific to nodule bacteria of legumes in the formation and functioning of the nitrogen fixing complex. The Scientific Issues of Ternopil Volodymyr Hnatiuk National Pedagogical University. Series Biology, No. 3 (18), pp. 49-57 [in Ukrainian].

57. Malichenko, S.M., Mamenko, P.M. & Kots, S.Ya. (2002). Influence of various activity of strains of the genus Bradyrhizobium on the dynamics of lectin activity of the root tubes and the functioning of the nitrogen fixing apparatus of lupine. Fiziologia i biokhimia kult. rastenij, 34, No. 6, pp. 511-516 [in Ukrainian].

58. Malichenko, S.M., Omelchuk, S.V. & Zhemojda, A.V. (2012, January). Influence of pollinators on the growth of Mesorhizobium ciceri bacteria and the formation of symbiosis in chickpea plants. Materials of the IV open congress of phytobiologists of the Black Sea region (p. 44), Kherson [in Ukrainian].

59. Mamenko, P.M., Kots, S.Ya., Drozdenko, G.M. & Zhemojda, A.V. (2008). Protein composition of soybean nodules, inoculated strains and Tn5 mutants of Bradyrhizobium japonicum of various efficacy. Fiziologia i biokhimia kult. rastenij, 40, No. 6, pp. 525-531 [in Ukrainian].

60. Mamenko, P.M., Kots, S.Ya. & Yakymchuk, R.A. (2004). Dynamics of activity of lectins in different organs of lupine depending on the efficiency of the strain-inoculant. Fiziologia i biokhimia kult. rastenij, 36, No. 2, pp. 139-146 [in Ukrainian].

61. Mamenko, P.M., Malichenko, S.M., Datsenko, V.K. & Kots, S.Ya. (2003). Symbiotic properties and soybean productivity depending on the concentration of its lectin in the inoculum suspension. Fiziologia i biokhimia kult. rastenij, 35, No. 3, pp. 215-221 [in Ukrainian].

62. Mamenko, P.M. (2014). Symbiotic system formation and protein composition of soybean roots inoculated with Bradyrhizobium japonicum strains under different water supply. Fiziol. rast. genet., No. 3, pp. 236-244 [in Ukrainian].

63. Mandrovska, N.M., Krugova, O.D., Vasyliuk, V.M. & Kots, S.Ya. (2008). Catalase activity in the roots and nodules of soybean, inoculated by Tn5-mutants of Bradyrhizobium japonicum. The Bulletin of Ukrainian Society of Geneticists and Breeders, 6, No. 1, pp. 73-81 [in Ukrainian].

64. Mandrovska, N.M., Krugova, O.D. & Ohrimenko, S.M. (2007). Symbiotic properties and culture and biochemical features of Tn5 mutants of nodules bacteria of peas. Achievements and problems of genetics, breeding and biotechnology (pp. 360-366), Vol. 1, Kyiv: Logos [in Ukrainian].

65. Melnyk, V.M. & Ogir, A.D. (2014, June). Activity of guaiacol peroxidase in the roots of soya, inoculated Bradyrhizobium japonicum, for the effects of drought. Materials of the Intern. scientific-practical conf. of young scientists Problems and prospects of research of the plant world (p. 233), Yalta [in Ukrainian].

66. Melnyk, V.M. & Ogir, A.D. (2014, June). Formation and functioning of the symbiotic systems soybeanBradyrhizobium japonicum in conditions of water stress. Abstracts International sciences conf. Selection and genetics of legume crops: modern aspects and perspectives (pp. 259-261), Odessa [in Ukrainian].

67. Mel'nykova, N.M. (1997). Biochemical characterization of noduleless soybean lectins. Ukrain'skyi biokhimichnyi zhurnal, 69, No. 5-6, pp. 158-162 [in Ukrainian].

68. Melnykova, N.M., Mamenko, P.M. & Kots, S.Ya. (2004). Two haemagglutinating protein fractions with different carbohydrate specificity from non-nodulating soybean seeds. Dopov. Nac. Akad. Nauk. Ukr., No. 11, pp. 167-171 [in Ukrainian].

69. Melnykova, N.M., Mamenko, P.M., Omel'chuk, S.V. & Malichenko, S.M. (2005). Formation of soybean seedlings under the action of the lectins from nodulating and non-nodulating soybean seeds. Zhyvlenniya Roslyn: teoriya i praktyka. Kyiv: Logos, pp. 363-370 [in Ukrainian].

70. Melnykova, N.M., Melnyk, V.M., Mykhalkiv, L.M., Omelchuk, S.V., Kots S.Ya. & Yakimchuk, R.A. (2009). Formation of symbiosis in lupine inoculated by Tn5 mutants of Bradyrhizobium sp. (Lupinus) in field. Bull. Kharkiv Nat. Agrar. Univ. Ser. Biol., 1, pp. 52-57 [in Ukrainian].

71. Melnykova, N.M., Mykhalkiv, L.M. & Omel'chuk, S.V. (2009). Nitrogen fixation activity of the soybean-Bradyrhizobium japonicum symbiosis and plant biomass formation under the action of non-nodulating soybean seed lectin. Fiziologiya Roslyn: problemy i perspektyvy rozvytku. Kyiv: Logos, V. 1, pp. 492-496 [in Ukrainian].

72. Mel'nykova, N.M. (1997). Peculiarities of amino acid composition of lectin from noduleless soybean. Ukrain'skyi biokhimichnyi zhurnal, 69, No. 5-6, pp. 200-202 [in Ukrainian].

73. Melnykova, N.M. (2014). Formation of the legume-rhizobium symbiosis under influence of lupine seed exudates. The Scientific Issues of Ternopil Volodymyr Hnatiuk National Pedagogical University. Series Biology, 3, No. 60, pp. 131-134 [in Ukrainian].

74. Melnykova, N.M., Kovalchuk, N.V., Kots, S.Ya. & Musatenko, L.I. (2009). Influence of soybean seeds lectins on the legume-rhizobium symbiosis formation and functioning. Fiziologia i biokhimia kult. rastenij, 41, No. 5, pp. 439-446 [in Russian].

75. Melnykova, N.M. & Kots, S.Ya. (2015). Effect of binary mixtures composed of soybean and pea lectins on the establishment of the soybean-rhizobium symbiosis. Bull. Kharkiv Nat. Agrar. Univ. Ser. Biol., 2 (35), pp. 64-71 [in Russian].

76. Mel'nikova, N.N. & Omel'chuk, S.V. (2009). Effect of legume seed exudates on the formation of Rhizobium-legume symbiosis. Applied Biochemistry and Microbiology, 45, No. 3, pp. 297-302. https://doi.org/10.1134/S0003683809030107

77. Mykhalkiv, L.M., Vorobey, N.A., Kots, S.Ya. & Sichkar, V.I. (2014, June). Efficiency of symbiosis of alfalfa with nodules bacteria, obtained by different methods. Abstracts International sciences conf. Selection and genetics of legume crops: modern aspects and perspectives (pp. 264-266), Odessa [in Ukrainian].

78. Mykhalkiv, L.M., Kots, S.Ya., Kiriziy, D.A., & Grigoryuk, I.P. (2001). Activation of CO2-gas exchange in alfalfa by synthetic cytokinins in conditions of water deficiency. Reports of the National Academy of Sciences of Ukraine, No. 12, pp. 141-146 [in Ukrainian].

79. Morgun, V.V. (2013). Scientific achievements of Institute of Plant Physiology and Genetics of National Academy of Sciences of Ukraine. Fiziol. rast. genet., 45, No. 6, pp. 463-477 [in Ukrainian].

80. Patyka, V.P., Kots, S.Ya., Volkohon, V.V., Sherstoboeva, O.V., Melnychuk, T.M., Kalinichenko, A.V. & Hrynyk, I.V. (2003). Biological Nitrogen. V. P. Patyka (Ed.). Kyiv: Svit [in Ukrainian].

81. Sytnikov, D.M., Kots, S.Ya., Malichenko, S.M. & Kiriziy, D.A. (2006). Photosynthetic rate and lectin activity of soybean leaves after inoculation with rhizobia together with homologous lectin Plant Physiology, 53, No. 2, pp. 189-195 [in Russian]. https://doi.org/10.1134/S102144370602004X

82. Sytnikov, D.M., Kots, S.Ya. & Malichenko S.M. (2006). Lectin activity of different soybean organs under conditions of efficient and inefficient symbiosis. Fiziologia i biokhimia kult. rastenij, 38, No. 11, pp. 53-60 [in Russian]. https://doi.org/10.1134/S102144370602004X

83. Pat. 13298 UA, IPC CO5F11/08, C12№1/20. Culture of bacteria Rhizobium meliloti for production of bacterial fertilizer for lucerne, Starchenkov, Yu.P., Peterson, O.V., Yakovets, L.M., Kots, S.Ya., Publ. 28.02.1997 [in Ukrainian].

84. Pat. 21012 UA, IPC CO5F11/08, C12R1:41. Bacteria strain Rhizobium leguminosarum the preparation of fertilizer under pea, Starchenkov, Yu.P., Kruhova, O.D., Ostapenko, D.D., Krymska, O.M., Tsymbal, H.S., Publ. 27.02.1998 [in Ukrainian].

85. Pat. 21013 UA, IPC CO5F11/08, C12R1:41. Bacteria strain Rhizobium lupini for production of bacterial fertilizer for lupine, Starchenkov, Yu.P., Nichik M.M, Mandrovska, N.M., Kruhova, O.D., Publ. 27.02.1998 [in Ukrainian].

86. Pat. 21785 UA, IPC C12№1/20. A strain of bacteria of Rhizobium leguminosarum bv. trifolii / Vorobei, N.A., Kots, S.Ya., Publ. 10.04.2007, [in Ukrainian].

87. Pat. 50851 UA, CO5F11/08, C12№1/20. Culture of bacteria Rhizobium meliloti for production of bacterial fertilizer for lucerne, Kots, S.Ya., Vorobei, N.A., Starchenkov, Yu.P., Publ. 15.11.2002 [in Ukrainian].

88. Pat. 51890 UA, IPC CO5F11/08, C12№1/29 // (C12№1/20, C12R1:41). Strain of bacteria Rhizobium sp. (Galega) ms-1 №159 (Collection of IFRG NAS of Ukraine) to produce a bacterial fertilizer for goatpens, Malichenko, S.M., Kots, S.Ya., Tytova, L.V., Butnytskyi, I.M., Publ. 15.11.2004 [in Ukrainian].

89. Pat. 102077 UA, IPC A0125/00, A01№65/20. Applying soybean seed lectin to regulate forming and functioning alfalfa and soybean legume-rhizobial symbiosis of at drought, Kots, S.Ya., Mykhalkiv, L.M., Mamenko, P.M., Veselovska, L.I., Publ. 26.10.2015, [in Ukrainian].

90. Pat. 78755 UA, IPC CO5F11/00, C12№1/100. Bacteria strain Bradyrhizobium japonicum PC08 (B-7399) for the preparation of bacterial fertilizer under soybean, Kots, S.Ya., Malichenko S.M., Mamenko, P.M., Vorobei, N.A., Yakymchuk, R.A., Publ. 25.03.13, [in Ukrainian].

91. Pat. 95714 UA, IPC CO5F11/08, C12№1/20, C12R 1/41A01 № 62/02б A01P 21/00. Strain of bacteria Rhizobium galegae Л2 (Collection of IFRG NAS of Ukraine) to produce a bacterial fertilizer for goatpens, Kyrylenko, L.V., Kots, S.Ya., Mamenko, P.M. Publ. 12.01.15, [in Ukrainian].

92. Pat. 102763 UA, IPC 2015.01, A01N25/00 A01P21/00. A method for increasing the nitrogen-fixing activity and productivity of symbiotic systems alfalfaSinorhizobium meliloti, Kots, S.Ya., Mykhalkiv, L.M., Beregovenko, S.K. Publ. 25.11.15, [in Ukrainian].

93. Albrecht, S.L., Bennet, J.M. & Quessenberry, K.H. (1981). Growth and nitrogen fixation of Aeschynomene under water stressed conditions. Plant Soil., No. 60, pp. 113-133. https://doi.org/10.1007/BF02374115

94. Atkins, C.A. & Smith, P.M.C. (2007). Translocation in legumes: Assimilates, nutritients and signaling molecules. Plant Physiol., 144, No. 2, pp. 550-561. https://doi.org/10.1104/pp.107.098046

95. Cooper, J.E. (2007). Early interactions between legumes and rhizobia: Dislosing complexity in a molecular dialogue. J. Appl. Microbiol.,103, No. 5, pp. 1355-1365. https://doi.org/10.1111/j.1365-2672.2007.03366.x

96. Hartwig, U.A., Joseph, C.M. & Philips, D.A. (1991). Flavonoids released naturally from alfalfa seeds enhance growth rate of Rhizobium meliloti. Plant Physiol., No. 95, pp. 797-803. https://doi.org/10.1104/pp.95.3.797

97. Kondratiuk, Iu., Mamenko, P. & Kots, S. (2013). Quantitative changes in protein content of symbiotic systems of soybean under inoculation by rhizobia with different effectiveness and varied nitrogen providing. Natura Montenegrina, 12, No. 2, pp. 531-537.

98. Kots, S., Mykhalkiv, L. & Mamenko, P. (2008, August). The influence of legume lectin on alfalfa  Sinorhizobium meliloti symbiosis productivity. Book of Abstract 38th Ann. Meet. of the ESNA (p. 125), Krakow.

99. Kots, S.Ya., Mykhalkiv, L.M., Mamenko, P.M. & Volkogon, M.V. (2011). The study of alfalfa - Sinorhizobium meliloti symbiosis productivity under different water conditions and the influence of the legume seed lectin. J. Agricult. Sci. Technol., No. 3, pp. 454-457.

100. Kots, S.Y., Mykhalkiv, L.M. & Melnykova, N.M. (2003). Improving lucerne productivity under water stress by treatment with periplasmic glucan. Grassland Science in Europe, No. 8, pp. 649-652.

101. Kovalchuk, N.V., Melnykova, N.M. & Musatenko, L.I. (2012). Role of phytolectin in the life cycle of plants. Biopolym. Cell, 28, No. 3, pp. 171-180. https://doi.org/10.7124/bc.00004A

102. Kyrychenko, O.V. & Perkovska, G.Yu. (2007). Effect of hapten, N-acetyl-D-glucosamin, on biological activity of the wheat lectin. Gen. Appl. Plant Physiol., 33, No. 3-4, pp. 141-154.

103. Levishko, A., Mamenko, P. & Kots, S. (2014, September). Metabolic changes in soybean roots inoculated by Bradyrhizobium japonicum strains. FEBS J. Special Issue: FEBS EMBO 2014 Conf. (p. 582.), Paris.

104. Melnykova, N.M., Malichenko, S.M. & Kots, S.Ya. (2003). Protein composition and carbohydrate-binding activity of non-nodulating soybean root extract. Proc. 12th Intern. Congr. on Genes, Gene families, and Isozymes (Eds. C. Schnarrenberger, B. Wittmann-Liebold), (pp. 261-264), Medimond.

105. Melnykova, N.M., Mykhalkiv, L.M., Mamenko, P.M. & Kots, S.Ya. (2013). The areas of application for plant lectins. Biopolym. Cell, 29, No. 5, pp. 357-366. https://doi.org/10.7124/bc.00082A

106. Melnykova, N., Mykhalkiv, L., Omelchuk, S. & Kots, S. (2006, September). Effect of soybean seed exudates on soybean  Bradyrhizobium symbiosis formation. Proc. of 36th Ann. Meet. of the ESNA (pp. 321-324), Iasi: Ion Ionesku de la Brad.

107. Zahran, H. H. & Sprent, J.I. (1986). Effect of sodium chloride and polyethyleneglycol on root hair infection and nodulation of Vicia faba L. plants Rhizobium leguminosarum. Planta, No. 167, pp. 303-307. https://doi.org/10.1007/BF00391332