Fiziol. rast. genet. 2016, vol. 48, no. 2, 158-166, doi:

Nitric oxide and hydrogen peroxide as signal mediators at induction of heat resistance of wheat plantlets by exogenous jasmonic and salicylic acids

Karpets Yu.V.1, Kolupaev Yu.E.1, Kosakivska I.V.2

  1. V.V. Dokuchayev Kharkiv National Agrarian University p/o «Communist-1», Kharkiv, 62483, Ukraine
  2. M.G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine 2 Tereschenkivska St., Kyiv, 01601, Ukraine

The involvement of nitric oxide (NO) and hydrogen peroxide in the induction of heat resistance of wheat (Triticum aestivum L.) plantlets under the influence of exogenous stress phytohormones — jasmonic (JA) and salicylic (SA) acids — have been investigated. 24-hour treatment of plantlets with the JA and SA in concentration of 1 and 10 mM invoked the increase of their survival after the action of potentially lethal heat stress (46 °C, 10 mines). Under the influence of both phytohormones the small transitional increase of hydrogen peroxide content took place in roots of plantlets with the maximum in 30 min after the treatment beginning. The raise of nitric oxide content under the influence of JA and SA was more essential and continued during 2 hours from the moment of beginning of plantlets treatment. The pretreatment with the antioxidant dimethylthiourea (DMTU) levelled the effect of increase of NO content in the roots, caused by JA and SA. At the preliminary influence of DMTU on plantlets, as well as at their pretreatment with the scavenger of nitric oxide PTIO (2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide) and the inhibitor of NO-synthase L-NAME (NG-nitro-L-arginine methyl ester), the development of heat resistance induced by the influence of JA and SA was considerably suppressed. The conclusion about the role of NO and hydrogen peroxide in the processes of JA- and SA-induced development of heat resistance of wheat plantlets was made.

Keywords: Triticum aestivum L., jasmonic acid, salicylic acid, nitric oxide, hydrogen peroxide, signals transduction, heat resistance

Fiziol. rast. genet.
2016, vol. 48, no. 2, 158-166

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