The free proline and sucrose contents as well as monosugars/sucrose ratio were estimated in corn calli cultures. Calli were initiated from T1 corn plants, obtained via in planta Agrobacterium-mediated transformation with LBA4404 strain harboring pBi2E with double-stranded RNA-suppressor of the proline dehydrogenase gene (L-390-T1) and from inbred line L-390 plants (control). Calli cultures were examined under osmotic stresses (2 % sea water salts, 0,5 M mannitol) during 10 days. Under normal conditions free proline contents in cells of both variants were low but the sucrose level in L-390-T1 cells was 7,5 over than control data. After 10 days of stress pressure the differences among variants were marked. In control variants, cultivated under mannitol influence, the free proline level remained constant but the sucrose content increased in many times. Under salinity monosugars/sucrose ratio increased slightly while proline accumulation grew in 2,8 times over normal data. Normal cells developed various types of stress-dependent protection reactions. In L-390-T1 cells under any stress pressure the free proline level raised slightly, the carbohydrate ratio was identical. The transformed cell culture maintained normal function.
Keywords: Zea mays L., corn, calli culture, genetic transformation, dsRNA-suppressor of the proline dehydrogenase gene, salinity, water deficit, proline, sucrose, tolerance
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