С целью установления пригодности разработанной ранее методики микроклонального размножения для получения генетически однородного растительного материала D. antarctica с использованием ПЦР-анализа и цитогенетического анализа исследована генетическая изменчивость растений в процессе размножения и культивирования in vitro. Изучено клональное потомство растений, отличавшихся по молекулярно-генетическим маркерам и цитогенетическим характеристикам. Сравнительным анализом растений на начальных этапах размножения (1—6-й пассажи) и при длительном культивировании in vitro (24—26-й пассажи и более) не выявлены генетические различия между клонами общего происхождения и исходным генотипом по ISSR-маркерам. С помощью анализа длительно культивируемых растений (49—79-й пассажи) установлено также сохранение цитогенетических характеристик растений. В целом полученные результаты подтвердили, что разработанная методика обеспечивает сохранение генетических характеристик D. antarctica в процессе длительного культивирования in vitro и может быть использована для получения генетически однородного растительного материала.
Ключевые слова: Deschampsia antarctica, micropropagation, genome variation, PCR-analysis, cytogenetic analysis
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