The aim of the work was to establish the possibility to use the composition of the glycoside hydrolase complex to determine the genetic affinity of streptomycetes. The object of the study was information about the streptomycetes complexes of glycoside hydrolases, which is presented in the available Internet databases (KEGG, NCBI and CAZy). The CAZy database provides information about enzymes of 603 streptomycetes that are active relative to carbohydrates. The study of information on streptomycetes complexes of glycoside hydrolases was carried out by manual analysis. Computed analysis of primary structures of streptomycetes DNA was performed using BLAST. The study presents the results of the analysis of glycosidase complexes of Streptomyces albidoflavus (12 strains) and Streptomyces griseus (11 strains) groups. The existence of the patterns of composition of glycosidase complexes in the strains of one layer is established. The genetic affinity of streptomycetes has been proven by determining the identity indices of their 16S rRNA-genes using BLAST analysis. The existence of correlation of the composition of glycoside hydrolase complexes in streptomycetes from a certain laying, and their genetic affinity were established. Based on our results, the strains of S. coelicolor A3(2), S. violaceoruber S21 and S. graminofaciens A-8890 are not members of those groups to which they are classified at the moment. The need to attribute the strain of S. coelicolor A3 (2) to S. violaceoruber group previously proposed by other authors. According to our results, the S. violaceoruber S21 strain is a member of the S. griseus group. Thus, the studying of the glycoside hydrolase complexes of streptomycetes to determine their genetic affinity in addition to traditional phenotypic and genotypic criteria is promising.
Keywords: Streptomyces, GH family, 16S rRNA, similarity indices, genetic relatedness
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