Fiziol. rast. genet. 2022, vol. 54, no. 5, 419-428, doi: https://doi.org/10.15407/frg2022.05.419

Increase in the phytotoxic effect of protoporphyrinogen oxidase inhibiting herbicide carfentrazone and herbicide synthetic auxin 2,4-D by join use with the NO donor sodium nitroprusside

Ponomareva I.G., Khandezhyna M.V., Radchenko M.P.

  • Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine 31/17 Vasylkivska St., Kyiv, 03022, Ukraine

The influence of the NO donor sodium nitroprusside (SNP) on the phytotoxic effect of the herbicide protoporphyrinogen oxidase (PPO) inhibitor carfentrazone and the herbicide 2,4-D from the class of synthetic auxins was studied. The study of the effect of SNP on the action of herbicides was carried out in the conditions of greenhouse experiments using oil radish (Raphanus sativus L. var. oleifera Metzg.) plants as a model of annual dicotyledonous weeds sensitive to the action of the studied herbicides. Treatment with herbicides was carried out in phase 2 of true leaves of radishes by spraying plants with solutions of herbicides and SPN separately, with solutions of herbicides with the addition of SNP, inactivated SNP and urea. The phytotoxic effect was evaluated by inhibiting the increase in the fresh mass of the above-ground part of the plants. It was established that when plants were treated with SNP solution in concentrations of 2.5 and 5.0 mM, a tendency to growth stimulation was observed. The herbicide carfentrazone at rates of 15 and 20 g/ha very weakly, and the herbicide 2,4-D at the rate of 360 g/ha significantly inhibited the growth of oil radish. An increase in the inhibitory effect of carfentrazone at a rate of 15 g/ha was observed when combined with SNP at a concentration of 5.0 mM, and at a rate of carfentrazone application of 20 g/ha at both studied concentrations of SNP 2.5 and 5.0 mM. Under join application of 2,4-D with SNP, an increase in the inhibitory effect was observed only at a SNP concentration of 5.0 mM. Addition to the solution of inactivated SNP, which lost its ability to form NO due to exposure to light for 24 hours, and urea at a concentration of 15 mM, which is equivalent to the SNP solution at a concentration of 5.0 mM in terms of nitrogen content, did not affect the herbicides action. The latter is evidence that the increase in the inhibitory effect of carfentrazone and 2,4-D by join application with SNP is due to its ability to be a NO donor. The obtained results proved the fundamental possibility of increasing the phytotoxic effect of PPO inhibitor herbicides and synthetic auxins due to the simultaneous use with the NO donor.

Keywords: NO, sodium nitroprusside, herbicides, carfentrazone. 2,4-D

Fiziol. rast. genet.
2022, vol. 54, no. 5, 419-428

Full text and supplemented materials

Free full text: PDF  

References

1. Kraehmer, H., Laber, B., Rosinger, C. & Shulz, A. (2014). Herbicides as weed control agents: state of the art: I. Weed control research and safener technology: the path to modern agriculture. Plant Physiology, 166, pp. 1119-1131. https://doi.org/10.1104/pp.114.241901

2. Morderer, Y.Y., Radchenko, M.P. & Sychuk, A.M. (2013). Programmed cell death in the pathogenesis, induced by herbicides in plants. Fiziol. rast. genet., 45, No. 6, pp. 517-526 [in Ukrainian] http://dspace.nbuv.gov.ua/handle/123456789/159373

3. Oz, M.T., Eyidogan, F., Yucel, M. & љktem, H.A. (2015). Functional role of nitric oxide under abiotic stress conditions. In Khan, M.N., Mobin, M., Mohammad, F. & Corpas, F.J. (Eds.). Nitric oxide action in abiotic stress responses in plants. Switzerland: Springer (pp. 21-42). https://doi.org/10.1007/978-3-319-17804-2_2

4. Parankusam, S., Adimulam, S.S., Bhatnagar-Mathur, P. & Sharma, K.K. (13.09.2017) Nitric Oxide (NO) in Plant Heat Stress Tolerance: Current Knowledge and Perspectives. Front. Plant Sci, e1582. Retrieved from https://doi.org/10.3389/fpls.2017.01582

5. Sami, F., Faizan, M., Faraz, A., Siddiqui, H., Yusuf, M. & Hayat, S. (2018). Nitric oxide-mediated integrative alterations in plant metabolism to confer abiotic stress tolerance, NO crosstalk with phytohormones and NO-mediated post translational modifications in modulating diverse plant stress. Nitric Oxide, 28, No. 73, pp. 22-38. https://doi.org/10.1016/j.niox.2017.12.005

6. Keshavarz-Tohid, V., Taheri, P., Taghavi, S.M. & Tarighi, S. (2016). The role of nitric oxide in basal and induced resistance in relation with hydrogen peroxide and antioxidant enzymes. Journal of Plant Physiology, 199, pp. 29-38. https://doi.org/10.1016/j.jplph.2016.05.005

7. Karpetz, Yu.V. (2019). Donors of nitric oxide and their application for increase in plant resistance to action of abiotic stressors. Visn. Hark. nac. agrar. univ., Ser. Biol., 48, Is. 3, pp. 28-51. [in Ukrainian] https://doi.org/10.35550/vbio2019.03.028

8. Pedroso, M.C., Magalhaes, J.R. & Durzan, D.J. (2000). Nitric oxide induces cell death in Taxus cells. Plant Sci. 157, Is. 2, pp.173-180. https://doi.org/10.1016/S0168-9452(00)00278-8

9. Clarke, A., Desikan, R., Hurst, D.R., Hancock, J.T. & Neill, S.J. (2000). NO way back: nitric oxide and programmed cell death in Arabidopsis thaliana suspension cultures. The Plant Journal, 24, No. 5, pp. 667-677. https://doi.org/10.1046/j.1365-313x.2000.00911.x

10. Li, Z.-C., Ren, Q.-W., Guo, Y., Ran, J., Ren, X.-T., Wu, N.-N., Xu, H.-Y., Liu, X. & Liu, J.-Z. (10 February 2021) Dual Roles of GSNOR1 in Cell Death and Immunity in Tetraploid Nicotiana tabacum. Front. Plant Sci. e596234. https://doi.org/10.3389/fpls.2021.596234

11. De Michele, R., Vurro, E., Rigo, C., Costa, A., Elviri, L., Di Valentin, M., Careri, M., Zottini, M., di Toppi, L.S. & Lo Schiavo, F. (2009). Nitric Oxide Is Involved in Cadmium-Induced Programmed Cell Death in Arabidopsis Suspension Cultures. Plant Physiology, 150, No. 1, pp. 217-228. https://doi.org/10.1104/pp.108.133397

12. Hung, K.T., Chang, C.J. & Kao, C.H. (2002). Paraquat toxicity is reduced by nitric oxide in rice leaves. J. Plant Physiol., 159, No. 2, pp. 159-166. https://doi.org/10.1078/0176-1617-00692

13. Ferreira, L.C., Cataneo, A.C., Remaeh, L.M., Coriani, N., Fumis, T., Soyza, Y.A., Scavroni, J. & Soares, B.J. (2010). Nitric oxide reduces oxidative stress generated by lactofen in soybean plants. Pesticide biochemistry and physiology, 97, No. 1, pp. 47-54. https://doi.org/10.1016/j.pestbp.2009.12.003

14. Singh, H., Singh, N.B., Singh, A., Hussain, I. & Yadav, V. (2017). Physiological and biochemical roles of nitric oxide against toxicity produced by glyphosate herbicide in Pisum sativum. R. J. Plant Physiol., 64, No. 4, pp. 518-524. https://doi.org/10.1134/S1021443717040136

15. Sychuk, A.M., Radchenko, M.P. & Morderer, Y. (2013). The increaseof phytotoxic action of graminicide fenoxaprop-p-ethyl by NO donor sodium nitroprusside. Science and Educationa New Dimension: Natural and Technical Sciences, I (2), No. 15, pp. 21-22.

16. Sychuk, A.M. (2015). The participation of programmed cell death in the herbicides induced pathogenesis. Thesis for PhD sci. degree in biological sci., spec. 03.00.12. Plant Physiology. Institute of Plant Physiology and Genetics. Kyiv, Ukraine [in Ukrainian].

17. Schwartau, V.V. (2009). Herbicides. The base of phytotoxicity regulation and physical, chemical and biological properties. Vol. 2. Kyiv: Logos [in Ukrainian].

18. Radchenko, M.P., Ponomareva, I.G., Pozynych, I.S. & Morderer, Ye.Yu. (2021). Stress and use of herbicides in field crops. Agriculture Science and Practice, 8, No. 3, pp. 50-70. https://doi.org/10.15407/agrisp8.03.050

19. Delledonne, M., Zeier, J., Marocco, A. & Lamb, C. (2001). Signal interactions between nitric oxide and reactive oxygen intermediates in the plant hypersensitive disease resistance response. Proc. Natl. Acad. Sci. USA, 98, pp. 13454-13459. https://doi.org/10.1073/pnas.231178298

20. Wang, Y., Loake, J.G. & Chu, C. (2013). Cross-talk of nitric oxide and reactive oxygen species in plant programed cell death. Front Plant Sci. Sec. Plant Physiology, e314. https://doi.org/10.3389/fpls.2013.00314