The aim of the study was to develop an effective method of in vitro cultivation of cherry cultivar Gisela 5 (Prunus cerasus ´ Prunus canescens), which is a valuable rootstock variety in the climatic conditions of Ukraine, using the method of adding liquid nutrient medium at the stage of elongation and further rooting stage. The effect of adding liquid nutrient medium (Quorin & Lepoivre — QL) with gibberellic acid (GA3) at three concentrations (0.5 mg/l, 1.0 mg/l and 1.5 mg/l) when added on the third week of cultivation on the medium for multiplication was studied with subsequent cultivation for two weeks. Also, the effect of pre-addition of liquid nutrient medium on the effectiveness of subsequent rooting was investigated. It was shown that the addition of 5 ml of liquid nutrient medium with 1 mg/l GA3 was the most effective, with a high number of plants obtained for rooting. In the case of GA3 1.5 mg/l the number of shoots with necrotic manifestations and signs of hyperhydration (vitrification) increased, such shoots are unsuitable for further rooting or multiplication. It was supposed that the reason for this was the increase in humidity in the container with plants, and the high concentration of GA3. The dependence of rooting on the previous addition of liquid nutrient medium with GA3 was evaluated, with an increase in total roots, root length and total number of rooted plants compared to the control, which in turn will improve further adaptation of plants to ex vitro conditions, but a clear dependence on concentration GA3 was not observed.
Keywords: Gisela 5 cherry rootstock, in vitro, liquid nutrient media, elongation, multiplication, rooting, plant growth regulators, gibberellic acid
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