The effect of high temperature stress on the cytokinins content in the overground part and roots of winter rye (Secale cereale L.) plants of the Boguslavka variety was studied under conditions of pot experiment. Plants were grown from seeds for 7 days at an air temperature of +16 °С, stress was created by transferring them to a thermostat with a temperature of +35 °С for 2 h (alarm stage) or twice for 6 h for two days (acclimation stage). Plants were also analyzed 5 days after the end of stressor action (recovery stage). The cytokinins qualitative composition and quantitative content were investigated by high performance liquid chromatography on Agilent 1200 LC liquid chromatograph with diode-matrix detector G 1315 B (USA). Significant changes in morphometric parameters, which reflected the inhibition of growth processes in rye plants under heat stress, were detected after two days of the experiment, while the pool of endogenous cytokinins considerable changed after 2 h of hyperthermia. In particular, concentration of trans-zeatin and trans-zeatin riboside notably decreased in the overground part, and in the roots — increased. The accumulation of free cytokinins was observed in the overground part of rye seedlings after two days of exposure to heat stress, at the same time a decrease in their levels was recorded in the roots. After a 5-day recovery period, the experimental plants differed from the control by a higher content of trans-zeatin and trans-zeatin riboside (approximately by 20 %) both in the overground part and in the roots. In general, studies have shown that hyperthermia has a differentiated effect on the content of individual cytokinins and their localization in the overground and underground parts of winter rye seedlings of Boguslavka variety. The detected fluctuations of cytokinins indicate their direct participation in the regulation of winter rye plants adaptation to the hyperthermia action.
Keywords: Sеcale cereale L., cytokinins, growth, hyperthermia, stress, adaptation
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