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ISSN 2308-7099
Plant Physiology and Genetics
 
 
Fiziol. rast. genet. 2020, vol. 52, no. 6, 483-493, doi: https://doi.org/10.15407/frg2020.06.483

Biochemical composition of seeds of transgenic spring rapeseed plants carrying the Mammalia cyp11a1 gene

Shishlova-Sokolovskaya A.M.1, Efimenko S.G.2

  1. State Scientific Institution «Institute of Genetics and Cytology of the National Academy of Sciences of Belarus»
    27 Akademicheskaya St., 220072 Minsk, Republic of Belarus
  2. Federal state budgetary scientific institution «Federal scientific center «V.S. Pustovoit All-Russian Research Institute of Oil Crops»»
    17 Filatova St., 350038 Krasnodar, Russian Federation

The qualitative and quantitative fatty-acid seed composition was identified by the gas chromatography method, and the Kjeldahl method was used to identify the total protein in the fresh mass in Т0—Т3 generations of transgenic spring rapeseed lines of Magnat variety, the Belarusian breeding. Transgenic lines were previously developed by Agrobacterium-mediated transformation using a construct carrying transcriptionally active heterologous genes: mammalian — cyp11a1 of cytochrome P450scc and bacterial — bar. Biometric analysis allowed to establish a stable increase in the mass of 1000 seeds and main raceme indices (length, the number of pods and side shoots) in these lines. Biochemical analysis showed that the insertion of the heterologous cyp11a1 gene does not affect the qualitative seed oil composition of transgenic lines, while significant changes were noted in the quantitative ratio of fatty acids in the seeds of transgenic lines in Т0—Т3 generations as compared to the control plants.

Keywords: Brassica napus L. var. oleifera DC., cyp11a1 gene, cytochrome P450scc, seeds, fatty acids, total protein

Fiziol. rast. genet.
2020, vol. 52, no. 6, 483-493

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