Using allele-specific primers and protein electrophoresis, in 37 soft wheat varieties the allelic composition of low molecular weight glutenin Glu-A3, Glu-B3, and high molecular weight glutenin Glu-A1, Glu-B1, Glu-D1 loci was determined. Two alleles were detected at the Glu-A3 locus: Glu-A3d, Glu-A3c (the Glu-A3c allele prevailed); four alleles were detected at the Glu-B3 locus: Glu-B3b, Glu-B3j, Glu-B3g, Glu-B3d (the allele Glu-B3b prevailed). Three alleles were detected at the Glu-A1 locus, the Glu-A1a allele prevailed. Five alleles were detected at the Glu-B1 locus: Glu-B1al, Glu-B1b, Glu-B1c, Glu-B1d, Glu-B1h (the Glu-B1c allele prevailed). Two alleles were detected at the Glu-D1 locus: the Glu-D1a, Glu-D1d (the Glu-D1d allele prevailed). To determine the dependence of wheat flour bakery quality on the allelic state of high molecular weight and low molecular weight glutenin loci, technological indices, caused by gluten proteins, were determined: flour sedimentation index, flour strength, dough elasticity index, dough tensility and resiliency index, strain factor, and dough elasticity and resiliency ratio. The dependence of flour baking quality on the allelic state of low molecular weight glutenins loci Glu-A3 and Glu-B3 was shown. It was found that the highest indices of sedimentation, flour strength and elasticity were characteristic of varieties with alleles Glu-B3b and Glu-B3g. In the varieties with Glu-A3c alleles, the sedimentation index, flour strength, elasticity and dough deformation indices were higher than in the samples with Glu-A3d allele. It has been confirmed that Glu-B1al allele of the high molecular weight glutenin Glu-B1 locus is characteristic of wheat with the highest flour baking quality.
Keywords: Triticum aestivum L., bread wheat, grain storage proteins, low molecular weight glutenin and high molecular weight glutenin loci, PCR analysis
Full text and supplemented materials
Free full text: PDFReferences
1. Morgun, V.V. (2016). Contribution of genetics and plant breeding to the food security of Ukraine. Visn. Nac. Akad. Nauk Ukr., 5, pp. 20-23 [in Ukrainian].
2. Morgun, V.V., Sanin, E.V. & Schwartau, V.V. (2015). Club 100 centners. Modern varieties and systems of winter wheat nutrition and protection. Kiev: Logos [in Ukrainian].
3. Morgun, V.V. & Rybalka, O.I. (2017) Strategy of cereals genetic improvement aimed at food safety, health promotion and industry needs. Visn. Nac. Akad. Nauk Ukr., 3, pp. 54-64 [in Ukrainian]. https://doi.org/10.15407/visn2017.03.054
4. Poperela, F.O. (1996). Three basic genetic systems for winter wheat soft wheat quality. Realization of potential possibilities of varieties and hybrids of the Breeding and Genetic Institute in Ukraine. Collection of Scientific Proceedings of SGI, pp.117-132 [in Ukrainian].
5. Gupta, R.B. & Shepherd, K.W. (1990). Two-step one-dimensional SDS-PAGE analysis of LMW subunits of glutenin: 1. Variation and genetic control of the subunits in hexaploid wheats. Theor. Appl. Genet, 80, pp. 65-74. https://doi.org/10.1007/BF00224017
6. Wurschum, T., Leiser, W., Kazman, E. & Longin, F. (2016). Genetic control of protein content and sedimentation volume in European winter wheat cultivars. Theor. Appl. Genet, 129, No. 3, pp. 1685-1696. https://doi.org/10.1007/s00122-016-2732-0
7. D'Ovidio, R., Fabbri, R., Patacchini, C., Masci, S., Lafiandra, D. & Porceddu, E. (2000). Production of transgenic bread wheat lines over-expressing a LMW glutenin subunit. Royal Society of Chemistry, pp. 101-104. https://doi.org/10.1039/9781847552372-00101
8. Li, Y., Zhou, R., Branlard, G. & Jia, J. (2010). Development of introgression lines with 18 alleles of glutenin subunit and evaluation of the effects of various alleles on quality related traits in wheat (Triticum aestivum L.). J. Cereal Sci., 51, pp. 127-133. https://doi.org/10.1016/j.jcs.2009.10.008
9. Ibba, M., Kiszonas, A. & Morris, C. (2017). Evidence of intralocus recombination at the Glu-3 loci in bread wheat (Triticum aestivum L.). Theor. Appl. Genet., 130, No. 5, pp. 891-902. https://doi.org/10.1007/s00122-017-2858-8
10. Branlard, G., Dardevet, M. & Saccomano, R. (2001). Genetic diversity of wheat storage proteins and bread wheat quality. Euphytica, 119, pp. 169-177. https://doi.org/10.1023/A:1017586220359
11. Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, No. 5259, pp. 680-685. https://doi.org/10.1038/227680a0
12. Payne, P.J. & Lawrence, G.J. (1983). Catalogue of alleles for the complex gene loci Glu-A1, Glu-B1, Glu-D1 which code for high-molecular-weight subunits of glutenin in hexaploid wheat. Gereal Res. Communs., 11, pp. 29-35.
13. Butow, B., Gale, K. & Ikea, J. (2004). Dissemination of the highly expressed Bx7 glutenin subunit (Glu-B1al allele) in wheat as revealed by novel PCR markers and RP-HPLC. Theor. Appl. Genet., 109, pp. 1525-1535. https://doi.org/10.1007/s00122-004-1776-8
14. Wang, L., Zhao, X. & He, Z. (2009). Characterization of low-molecular-weight glutenin subunit Glu-B3 genes and development of STS markers in common wheat (Triticum aestivum L.). Theor. Appl. Genet., 118, pp. 525-539. https://doi.org/10.1007/s00122-008-0918-9
15. Wang, L., Li, G. & Pena, R. (2010). Development of STS marker and establishment of multiplex PCR for Glu-A3 alleles in common wheat (Triticum aestivum L.). J. Cereal Sci., 51, pp. 305-312. https://doi.org/10.1016/j.jcs.2010.01.005
16. Zhang, W., Gianibelli, M. & Ma, W. (2003). Identification of SNPs and development of AS-PCR markers for gliadin alleles in Triticum aestivum. Theor. Appl. Genet., 107, pp.130-138. https://doi.org/10.1007/s00122-003-1223-2
17. Rybalka, O.I. (2011). Quality of wheat and it improvement. Kiev: Logos [in Ukrainian].
18. Tkachik, S.O. (Ed.) (2016). The technique of conducting a qualification examination of varieties of plant for appropriateness to extension in Ukraine. Methods of visibility indicators in productivity agriculture. Vinnytsia [in Ukrainian].
19. Radchenko, O.M. (2018). Polymorphism of soft wheat varieties by loci of low molecular weight glutenins. Fiziol. rast. genet., 50, No. 1, pp. 66-76 [in Ukrainian]. https://doi.org/10.15407/frg2018.01.066