en   ru   uk  
 
 
Fiziol. rast. genet. 2020, vol. 52, no. 3, 238-247, doi: https://doi.org/10.15407/frg2020.03.238

Barley locus sex6 mutation that substantionally improves nutritional grain values

Rybalka O.І.1,2, Кatrii V.B.2, Morgun B.V.2,3, Polyshchuk S.S.1

  1. Plant Breeding and Genetics Institute—National Center of Seed and Cultivars Investigation, National Academy of Agrarian Sciences of Ukraine 3 Ovidiopolska Road, Odesa, 65036, Ukraine
  2. Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine 31/17 Vasylkivska St., Kyiv 03022, Ukraine
  3. Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine 148 Akademika Zabolotnoho St., Kyiv, 03143, Ukraine

Hull-less barley cultivar with unique nutritional properties Himalaya 292 was developed by CSIRO (Australia) on the base of induced by sodium aside mutation in the sex6 locus. The mutation contains a stop-codon appeared as G to A transition and suppresses activity of the SSIIa starch granules bound enzyme responsible for branching of glucose polymer in the starch structure. As a result, the content of a non-branching glucose polymer (amylose) in the starch composition substantially increased from normal 20—25 % to high — 70 %. The original hull-less barley cultivar Himalaya 292 was examined in PBGI–NCSCI under field conditions for several years. The cultivar appeared to be not adapted, particularly to arid growing conditions. The plants were weak with 1-2 containing with small shrunken grains. From the binary cross Himalaya 292 w Datcha (Austria) on the base of F4/5 genotypes the experimental breeding population was created, among which 200 best genotypes were selected and subjected to the sex6 mutation detection by PCR coupled with restriction analysis and simultaneous grain amylose content examination. As the research performed, significantly high-amylose barley genotypes were selected for the further use in breeding program. The conclusion was made that the hull-less barley cultivar Himalaya 292 can be used exclusively as the genetic source of mutation in the sex6 locus for breeding program of adaptive to drought high amylose hull-less barley varieties development.

Keywords: hull-less barley, starch, amylose, mutation, locus sex6, starch synthase

Fiziol. rast. genet.
2020, vol. 52, no. 3, 238-247

Full text and suplimented materials

Free full text: PDF  

References

1. Englyst, K., Vinoy, S., Englyst, H. & Lang, V. (2003). Glycaemic index of cereal products explained by their content of rapidly and slowly available glucose. Br. J. of Nutr., 89, pp. 329-339. https://doi.org/10.1079/BJN2002786

2. Jenkins, D., Wolever, T. & Taylor, R. (1981). Glycemic index of foods: a physiological basis of carbohydrate exchange. Am. J. Clin. Nutr., 134, pp. 362-366. https://doi.org/10.1093/ajcn/34.3.362

3. Shi, Y-Ch. & Maningat, C. (2013). Resistant starch: sources, applications and health benefits. ISBN: 978-1-118-52875-4 Wiley-Blackwell. https://doi.org/10.1002/9781118528723

4. Rybalka, O., Morgun, B. & Polishchuk, S. (2016). Barley as a product of functional nutrition. Kyiv, Logos [in Ukrainian].

5. Morell, M., Kosar-Hashemi, B., Cmiel, M., Samuel, M., Chandler, P., Rahman, S., Buleon, A., Batey, I. & Li, Z. (2003) Barley sex6 mutants lack starch synthase IIa activity and contain a starch with novel properties. Plant J., 34, pp. 173-185. https://doi.org/10.1046/j.1365-313X.2003.01712.x

6. Zwar, J.A. & Chandler, P.M. (1995). a-Amylase production and leaf protein synthesis in a gibberellin-responsive dwarf mutant of 'Himalaya' barley (Hordeum vulgare L.). Planta, 197, pp. 39-48. https://doi.org/10.1007/BF00239937

7. Topping, D., Morell, M., King, R., Li, Z., Bird, A. & Noakes, M. (2003). Resistant starch and health - Himalaya 292, a novel barley cultivar to deliver benefits to consumers. Starch-Starke, 55, pp. 539-545. https://doi.org/10.1002/star.200300221

8. Bird, A., Flory, C., Davies, D., Usher, S. & Topping, D. (2004). A novel barley cultivar (Himalaya 292) with a specific gene mutation in starch synthesis IIa raises large bowel starch and short-chain fatty acids in rats. J. Nutr., 134, pp. 831-835. https://doi.org/10.1093/jn/134.4.831

9. Bird, A., Jackson, M., King, R., Davies, D., Usher, S. & Topping, D. (2004). A novel high-amylose barley cultivar (Hordeum vulgare var. Himalaya 292) lowers plasma cholesterol and alters indices of large-bowel fermentation in pigs. Br. J. Nutr., 92, pp. 607-615. https://doi.org/10.1079/BJN20041248

10. Redzhina, A., Morell, M. & Rakhman, S. (2007). Barley with varied activity of branching enzyme and starch and starch-containing products with increased amylose content. Patent RU 2303870 C2. Retrieved from http://patentimages.storage.googleapis.com /6a/83/39/198277ffd919b4/RU2303870C2.pdf

11. Mohammadkhani, A. (2005). Survey of starch amylose content in naked barley (H. vulgare nudum). Pakistan J. of Nutrition, 4, pp. 183-186. DOI: 10.3923/pjn.2005.183.186 https://doi.org/10.3923/pjn.2005.183.186

12. Lee, Bruce. (2009). The BARLEYmax Better Nutritional Report. Public Health Association Australia. CSIRO, Australia.

13. Li, Zhongyi. (2009). BARLEYmax™ Better Nutritional Report. CSIRO, Australia.

14. Bird, A., Vuaran, M., King, R., Noakes, M., Keogh, J., Morell, M. & Topping, D. (2008). Wholegrain foods made from a novel high-amylose barley variety (Himalaya 292) improve indices of bowel health in human subjects. Br. J. of Nutr., 99, pp. 1032-1040. https://doi.org/10.1017/S000711450783902X