The hydrogen sulfide donor sodium hydrosulfide (NaHS) influence on resistance of etiolated wheat (Triticum aestivum L.) seedlings to osmotic stress caused by 12 % PEG-6000 was investigated. Treatment with NaHS in concentrations 0.1, 0.5 and 1 mM reduced the growth inhibition of roods and shoots under the action of PEG and increased water content in seedlings. The most appreciable osmoprotective action of hydrogen sulfide donor was observed at 0.5 mM concentration. Under its influence at normal conditions and at osmotic stress there was activation of antioxidant enzymes namely catalase and guaiacol peroxidase, while activity of superoxide dismutase did not change significantly. Osmotic stress caused twofold increasing of proline content in shoots of seedlings, treatment with NaHS leaded to its more enhancing. Under the action of hydrogen sulfide donor the amount of anthocyanins in aboveground part of seedlings have risen at normal conditions and stabilized at stress ones. Seedlings treatment with NaHS leveled the oxidative stress caused by PEG, which evidenced in decreasing of hydrogen peroxide and malonic dialdehyde content in tissues. A conclusion was made that enhancing of resistance of wheat seedlings to osmotic stress, caused by H2S donor, related to activation of antioxidant and osmoprotective systems.
Keywords: Triticum aestivum L., hydrogen sulfide, osmotic stress, antioxidant enzymes, proline, anthocyanins
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