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Fiziol. rast. genet. 2018, vol. 50, no. 5, 427-438, doi: https://doi.org/10.15407/frg2018.05.427

Direction of inherited changes in flax (Linum humile Mill.) under the action of new dimethyl sulfate derivatives

Tigova A.V., Soroka A.I.

  • Institute of Oil Crops, National Academy of Agrarian Sciences of Ukraine 1 Institutskaya St., Settl. Solnechnyy, Zaporozhye, 69093, Ukraine

Mutational variability underlies the development of the source material for plant breeding. The method of induced mutagenesis allows to expand genetic diversity of species by involving samples with mutations of genes and chromosomes in the breeding process. Induced mutants obtained by the treatment with a various mutagenic agents may subsequently turn into new varieties after careful selection or involvement in hybridization. The article shows that treatment with new chemical mutagens of the DG series, derived from dimethyl sulfate (DMS), resulted in a wide range of morphological and physiological mutations that were divided into five groups. The frequency of mutations and the direction of the action of DG mutagens are described in comparison with the basic substance and well-known ethyl methane sulfonate (EMS) mutagen. It was demonstrated that the mutagens of the DG series largely surpassed the original DMS mutagen according to the frequency of the induced changes, which indicates their effectiveness. It was determined that DG-9 mutagen was the most effective one to cause mutations in chlorophyll synthesis, DG-7 and DG-6 mutagens were the most effective to induce mutations in the structure of stalk, shoots and leaves, DG-2 mutagen — to cause mutations of the corona and anthers color, seed color, and mutations for physiological traits of growth and development.

Keywords: Linum humile Mill., flax, mutagenesis, chemical mutagen, dimethyl sulfate, ethyl methane sulfonate, mutation

Fiziol. rast. genet.
2018, vol. 50, no. 5, 427-438

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References

1. Gaevsky, N.A. (2002). Acquaintance with evolutionary genetics. Krasnoyarsk: State. Un-t [in Russian].

2. Korolev, K.P., Bogdan, V.Z. & Bogdan, T.M. (2016). Induced mutagenesis of flax (Linum usitatissimum L.) as a method of creating a new source material for priority directions in breeding. Bulletin of the Belorussian State Agricultural, 4, pp. 73-75 [in Russian].

3. Krotova, L.A. (2015). Chemical mutagenesis as a method of creating the initial material for the selection of soft wheat. Electronic scientific-methodical journal of the Omsk State Agrarian University, 2, No. 2, pp. 13-17 [in Russian].

4. Kudina, A.G. (2006). Chemical mutagens in the breeding of ornamental plants. Industrial Botany, 6, pp. 116-120 [in Russian].

5. Lazareva, N.V. & Levina E.N. (1976). Hazardous substances in industry. Vol. 2. Leningrad: Chemistry [in Russian].

6. Lebedeva, O.N., Nikolaevskaya, T.S. & Titov, A.F. (2009). The load of pigment mutations and the survival of plants in the progenies of Festuca pratensis Huds., formed on a mutant basis. Proceedings of the Karelian Research Center of the Russian Academy of Sciences, 3, pp. 56-66 [in Russian].

7. Littl, T.M. & Hills, F.D. (1981). Agricultural experimental business. Planning and analysis. Moscow: Kolos [in Russian].

8. Lyakh, V.A., Polyakova, I.A. & Soroka, A.I. (2009). Induced mutagenesis of oilseeds. Zaporozhye: Zap. Nat. Un-t [in Russian].

9. Morgun, V.V. & Logvinenko, V.F. (1995). Mutational breeding of wheat. Kiev: Nauk. dumka [in Russian].

10. Tigova, A.V. & Soroka, A.I. (2016). Influence of new chemical mutagens on plants of Linum humile Mill. in the M1 generation. Bulletin of Zaporozhye State University. Biological Sciences, 1, pp. 15-22.

11. Tigova, A.V. & Soroka, A.I. (2016). Variation of some morphometric characteristics in Linum humile Mill. under the action of new chemical mutagens in the M1 generation. Bulletin of the Institute of Oilseeds crops of the USSR, 22, pp. 35-42 [in Russian].

12. Tigova, A.V. & Soroka, A.I. (2017). Frequency and spectrum of mutations in flax (Linum humile Mill.) under the action of new dimethyl sulfate derivatives. Fiziol. rast. genet., 49, No. 6, pp. 521-532 [in Russian].

13. Deepthi, T. & Remesh, K. (2016). Impact of EMS induction on morphological, anatomical and physiological traits of Bhindi Abelmoschus esculentus (L.) Moench, International Journal of Recent Research in Life Sciences (IJRRLS), 3, pp. 4-11.

14. Kharkwal, M.C., Pandey, R.N. & Pawar, S.E. (2004). Mutation breeding for crop improvement. In Plant Breeding: Mendelian to Molecular Approaches (pp. 601-645), Dordrecht. https://doi.org/10.1007/978-94-007-1040-5_26

15. Luan, Y., Zhang, J., Gao, X. & An, L. (2007). Mutation induced by ethylmethanesulphonate (EMS), in vitro screening for salt tolerance and plant regeneration of sweet potato (Ipomoea batatas L.). Plant Cell, Tissue and Organ Culture, 88 (1), pp. 77-81. doi: https://doi: 10.1007/s11240-006-9183-2. https://doi.org/10.1007/s11240-006-9183-2

16. Mahla, H., Shekhawat, A. & Kumar, D. (2010). A study on EMS and gamma mutagenesis of clusterbean (Cyamopsis tetragonoloba (L.) Taub.). Plant Mutation Reports, 2, No. 2, pp. 28-32.

17. Rajarajan, D., Saraswathi, R., Sassikumar, D. & Ganesh, S. (2014). Fixation of lethal dose and effect of ethyl methane sulphonate induced mutagenesis in rice Adt (R) 47. Life Sciences Leaflets, 57, pp. 65-72.

18. Talebi, A., Talebi, Am. & Shahrokhifar, B. (2012). Ethyl methane sulphonate (EMS) induced mutagenesis in malaysian rice (cv. MR219) for lethal dose determination. Am. J. of Plant Sci., 3, pp. 1661-1665. doi: https://doi: 10.4236/ajps.2012.312202 https://doi.org/10.4236/ajps.2012.312202

19. Tejklova, E. (2002). Curly stem — an induced mutation in flax (Linum usitatissimum L.). Czech J. Genet. Plant Breed, 38, No. 3-4, pp. 125-128. doi: https://doi.org/ 10.17221/6246-CJGPB

20. Wasserman, L. (2005). All of Statistics: A Concise Course in Statistical Inference. Springer.