Fiziol. rast. genet. 2018, vol. 50, no. 5, 419-426, doi: https://doi.org/10.15407/frg2018.05.419

The investigation Deutzia Thunb. genus stomata in relation whith their drought resistance in conditions of the northen part of the forest-steppe of Ukraine

Krugliak Yu.M.

  • M.M. Gryshko National Botanical Garden, National Academy of Sciences of Ukraine 1 Tymiryazevska St., Kyiv, 01014, Ukraine

The results of the study of the size and density of stomata of plants of such species of the Deutzia Thunb. genus from the collection of arboretum M. M. Gryshko National Botanical Garden of the NAS of Ukraine: D. w elegantissima (Lemoine) Rehd., D. gracilis Sieib. et Zucc., D. w magnifica (Lemoine) Rehd., D. w rosea (Lemoine) Rehd., D. scabra Thunb., D. scabra ‘Candidissima’, D. scabra ‘Plena’, D. schneideriana Rehd., D. longifolia ‘Sessiliflora’ are presented. Plants of the Deutziа genus are not native for Ukraine. An important index of the adaptation of alien plants to new environmental conditions is their ability to withstand arid periods of the year. One of the methods that helps to determine the potential drought tolerance of plants is studying of the size of stomata and their number per area unit of the leaf surface. Xerophitic plants have more stomata per unit area of the leaf blade and their size is smaller. The aim of the work was to determine the indices of stomata of plants of the Deutziа genus and to estimate drought tolerance of plants depending on the size of the stomata and their density. The state of stomatal apparatus was determined by making of epidermal imprints. The stomata was seen under a Primo Star light microscope with zoom of w40. To capture the actual material a Canon PowerShot A640 digital camera equipped with a microscope was used. Measurements of length and width of stomata were did on a computer using the license program Axio Vision Release 4.7. According to morphometric characteristics of the stomata the most resistant to drought are D. schneideriana and D. scabra ‘Candidissima’ because they have smaller sizes of stomata (16,78±0,29 w w 6,76±0,18 and 17,99±0,51 w 7,37±0,25 mm accordingly) and more quantity of stomata in mm2 (155,92±6,78 and 175,38±7,55 accordingly). Potentially the least resistance at arid period of the year have plants of D. w elegantissima і D. scabra. The sizes of their stomata are one of the largest and the density – one of the smallest.

Keywords: Deutzia Thunb., stomata, introduction, alien plants, drought resistance

Fiziol. rast. genet.
2018, vol. 50, no. 5, 419-426

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References

1. Kohno, M.A., Trofimenko, N.M., Parhomenko, L.I., Sobko, V.G., Gorb, V.K., Klimenko, S.V., Grevcova, G.T., Galkin, S.I, Muzika, G.I., Schepicka, T.S., Demchenko, O.O., Bilyk, O.V., Bonyuk, Z.G., Balabushka, V.K., Galushko, R.V., Gaponenko, M.B., Klimenko, Yu.O., Kolesnichenko, O.M., Sidoruk, T.M., Klyuyenko, O.V., Kornijchuk, V.S., Strila, T.Ye., Fedorovskij, V.D., Yadrov, A.A. & Kurdyuk, O.M. (2005). Dendroflora of Ukraine. Wild and cultivated trees and shrubs. Angiosperms. pt. II. M.A. Kohno, N.M. Trofymenko (Eds.). Kyiv: Fitosociocentr [in Ukrainian].

2. Zaikonnikova, T.I. (1966). Deutzia — ornamental shrubs. Moskva; Lenyngrad: Nauka [in Russian].

3. Flora of China. Missouri BGP (2001), Vol. 8, pp. 395-403.

4. Hetherington, A.M. & Woodward, F.I. (2003). The role of stomata in sensing and driving environmental change. Nature, No. 424 (6951), pp. 901-908. doi: 10.1038/nature01843. Retrived from https://www.ncbi.nlm.nih.gov/pubmed/12931178 https://doi.org/10.1038/nature01843

5. Camargo, M.A. & Marenco, R.A. (2011). Density, size and distribution of stomata in 35 rainforest tree species in Central Amazonia. Acta Amazonica, 41, No. 2. doi: http://dx.doi.org/10.1590/S0044-59672011000200004. Retrived from http://scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672011000200004 https://doi.org/10.1590/S0044-59672011000200004

6. Dittberner, H., Korte, A., Mettler-Altmann, T., Weber, A., Monroe, G. & de Meaux, J. (2016). Natural variation in stomata size contributes to the local adaptation of water-use efficiency in Arabidopsis thaliana. Vitis, No. 55, pp. 17-22. doi:https:// doi.org/10.1101/25302. Retrived from https://www.biorxiv.org/content/early/ 2018/01/24/253021

7. Procenko, D.P. & Brajon, O.V. (1981). Plant anatomy. Kyiv: Vyshha shkola [in Ukrainian].

8. Slejcher, R. (1970). Vodnyj rezhim rartenij. Moskva: Mir [in Russian].

9. Lawson, T., von Caemmerer, S. & Baroli, I. (2011). Photosynthesis and stomatal behaviour. Progress in Botany, No. 72, pp. 265-304. doi: https://doi.org/10.1007/978-3-642-13145-5_11. Retrived from https://www.researchgate.net/publication/ 227231189_Photosynthesis_and_Stomatal_Behaviour

10. Lawson, T. & Blatt, M.R. (2014). Stomatal size, speed, and responsiveness impact on photosynthesis and water use efficiency. Plant Physiology, 164, pp. 1556-1570. doi: https://doi.org/10.1104/pp.114.237107. Retrived from http://www.plantphysiol.org/content/164/4/1556#abstract-2 https://doi.org/10.1104/pp.114.237107

11. Brodribb, T.J., McAdam, S.A., Jordan, G.J. & Field, T.S. (2009). Evolution of stomatal responsiveness to CO2 and optimisation of water-use efficiency among land plants. New Phytologist., No. 183, pp. 839-847. doi: https://doi.org/10.1111/j.1469-8137.2009.02844. Retrived from https://www.ncbi.nlm.nih.gov/pubmed/19402882

12. Brodribb, T.J. & McAdam, S.A. (2011). Passive origins of stomatal control in vascular plants. Science, No. 331 (6017), pp. 582-585. doi: https://doi.org/10.1126/science.1197985. Retrived from https://www.ncbi.nlm.nih.gov/pubmed/21163966 https://doi.org/10.1126/science.1197985

13. Aasamaa, K. & Sober, A. (2011). Stomatal sensitivities to changes in leaf water potential, air humidity, CO2 concentration and light intensity, and the effect of abscisic acid on the sensitivities in six temperate deciduous tree species. Environmental and Experimental Botany, No. 71, pp. 72-78. doi: https://doi.org/10.1016/j.envexpbot. 2010.10.013. Retrived from https://www.infona.pl/resource/bwmetal.element.elsevier-4f95044d-c20d-3a89-8437-ce36ff177568.

14. Lakin, G.F. (1990). Biometrics. Moskva: Vysshaja shkola [in Russian].

15. Lebedev, S.I. (1988). Plant Physiology. Moskva: Agropromizdat [in Russian].

16. Polevoj, V.V. (1989). Plant Physiology. Moskva: Vysshaja shkola [in Russian].

17. Volenikova, M. & Ticha, I. (2001). Insertion profiles in stomatal density and sizes in Nicotiana tabacum L. plantlets. Biologia Plantarum, No. 44, pp. 161-165. doi: https://doi.org/10.1023/A:1017982619635. Retrived from https://link.springer.com/article/10.1023%2FA%3A1027982619635

18. Pausheva, Z.P. (1988). Workshop on plant cytology. Moskva: Agropromizdat [in Russian].