Fiziol. rast. genet. 2018, vol. 50, no. 5, 410-418, doi:

In vitro cultivation of Silybum marianum L. belarusian and hungarian breeding

Kovzunova O.V., Reshetnikov V.N.

  • State Scientific Institution «Central Botanical Garden of the National Academy of Sciences of Belarus» 2v Surganova St., Minsk, 220012, Belarus

The best way to sterilize the seeds of the spotted milk thistle, which is characterized by the maximum percentage of viable and sterile seeds (98—99 %) was determined. The dependence of the growth index on the combination of phytohormones in the culture medium was revealed. The optimum medium for obtaining callus tissue of spotted thistle red-flower variety Cinderella of the Belarusian breeding and the white-flower variety Sibilla of Hungarian breeding regardless of the origin of the explant, is the Murashige—Skoog medium containing 1 mg/l of 2.4-dichlorophenoxyacetic acid and 0.4 mg/l of kinetin.

Keywords: Silybum marianum L., in vitro culture, sterilization, callusogenesis, phytohormones, growth index

Fiziol. rast. genet.
2018, vol. 50, no. 5, 410-418

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