Increasing anthropogenic impact on the environment leads to a decrease in the biocenosis’ productivity. On the background of increasing use of mineral fertilizers with phosphate compounds by the agrarian sector and, consequently, soil contamination with cadmium micro additives, the use of nanoparticles of metals-micronutrients is considered as an alternative source of nutrition of important crops. The effect of pre-sowing treatment with a mixture of Cu, Zn, Mn, Fe nanoparticles and 0,1 mM Cd2+ on lettuce (Lactuca sativa L.) plants of two different in the phenolic metabolites accumulation varieties (phenotypically differing in the degree of pigmentation of the above-ground part — Lolo (green variety), Lolo Ross (red variety) — was investigated. It was studied the adaptive response of Lactuca sativa plants based on the analysis of the content of primary products of lipids peroxidation (hydroperoxides), and the activation of enzymatic (guaiacol-peroxidase and phenylalanine ammonia-lyase activity), and non-enzymatic (content of phenolic compounds) antioxidant systems. The specificity of the influence of essential metal nanoparticles and high content of cadmium has been determined. An additional to metal nanoparticles load with cadmium resulted in the accumulation of phenolic metabolites (36 %) and phenylalanine ammonia-lyase activity increase (twice) in the green variety Lolo, compared to the red Lolo Ross variety. However, both varieties showed a decrease in the level of lipids peroxidation products at the end of the exposition. It was established the absence of the leveling effect of nano-treatment on the toxic cadmium effect in both varieties.
Keywords: Lactuca sativa L., cadmium ions, essential metal nanoparticles, lipid hydroperoxides, guaiacol peroxidase, phenolic compounds, phenylalanine ammonia-lyase
Full text and supplemented materialsFree full text: PDF
1. Batsmanova, L.M. & Taran, N.Yu. (2010). Screening of plant adaptive capacity by indicators of oxidative stress. Kyiv: TOV Aveha [in Ukrainian].
2. Kosyk, O.I., Khomenko, I. M., Taran, N.Yu., Aidosova, S.S. & Sarsenbaev, K.N. (2016). Resistance features of mono- and dicotyledonous plants under the action of xenobiotics. ScienceRise: Biological Science, 3, iss. 3, pp. 37-45 [in Russian].
3. Azzi, V., Kazpard, V., Lartiges, B., Kobeissi, A., Kanso, A. & El Samrani, A. G. (2017). Trace metals in phosphate fertilizers used in Eastern Mediterranean countries. Clean Air Soil Water, 45, No. 1. doi: https://doi.org/10.1002/clen.201500988
4. Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, pp. 248-254. https://doi.org/10.1016/0003-2697(76)90527-3
5. Cuypers, A., Smeets, K., Ruytinx, J., Opdenakker, K., Keunen, E., Remans, T., Horemans, N., Vanhoudt, N., Van Sanden, S., Van Belleghem, F., Guisez, Y., Colpaert, J. & Vangronsveld, J. (2011). The cellular redox state as a modulator in cadmium and copper responses in Arabidopsis thaliana seedlings. J. Plant Physiol., 168, pp. 309-316. https://doi.org/10.1016/j.jplph.2010.07.010
6. Dias, A. & Kosta, M. (1983). Effect of low salt concentrations on nitrate reductase and peroxidase of sugar beet leaves. J. Exp Bot., 34, pp. 537-543. https://doi.org/10.1093/jxb/34.5.537
7. Eghbaliferiz, S. & Iranshahi, M. (2016). Prooxidant activity of polyphenols, flavonoids, anthocyanins and carotenoids: Updated review of mechanisms and catalyzing metals. Phytother. Res., 30, pp. 1379-1390. doi: https://doi.org/10.1002/ptr.5643
8. Fedenko, V.S., Shemet, S.A. & Struzhko, V.S. (2005). Complexation of cyanidin with cadmium ions in solution. Ukrainian Biochem J., 77, No. 1, pp. 104-109.
9. Ghassemzadeh, F., Yousefzadeh, H. & Arbab-Zavar, M.H. (2008). Antioxidative and metabolic responses to arsenic in the common reed (Phragmites australis): implications for phytoremediation. Land Contam. Reclam., 16, pp. 213-222. https://doi.org/10.2462/09670513.872
10. Juбrez-Maldonado, A., Gonzбlez-Morales, S. & Cabrera-De la Fuente, M. (2018). Nanometals as promoters of nutraceutical quality in crop plants. Impact of Nanoscience in the Food Industry (pp. 277-310). Academic Press. doi: https://doi.org/10.1016/B978-0-12-811441-4.00010-8
11. Karimi, J. & Mohsenzadeh, S. (2017). Physiological effects of silver nanoparticles and silver nitrate toxicity in Triticum aestivum. Iranian Journal of Science and Technology, 41, No. 1, pp. 111-120. doi: https://doi:10.1007/s40995-017-0200-6 https://doi.org/10.1007/s40995-017-0200-6
12. Konotop, Ye.O., Kovalenko, M.S., Ulynets, V.Z., Meleshko, A.O., Batsmanova, L.M. & Taran, N.Yu. (2014). Phytotoxicity of colloidal solutions of metal-containing nanoparticles. Cytology and Genetics, 48, No. 2, pp. 37-42. doi: https://doi.org/10.3103/S0095452714020054
13. Kosyk, O.I., Khomenko, I.M., Batsmanova L.M. & Taran, N.Yu. (2017). Phenylalanine ammonia-lyase activity and anthocyanin content in different varieties of lettuce under the cadmium influence. The Ukrainian Biochemical Journal, 89, No. 2, pp. 85-91. doi: https://doi:10.15407/ubj89.02.085 https://doi.org/10.15407/ubj89.02.085
14. Liu, R., Zhang, H. & Lal, R. (2016). Effects of stabilized nanoparticles of copper, zinc, manganese, and iron oxides in low concentrations on lettuce (Lactuca sativa) seed germination: Nanotoxicants or nanonutrients? Water Air Soil Pollut., 227, No. 1, p. 42. doi: https://doi: 10.1007/s11270-015-2738-2 https://doi.org/10.1007/s11270-015-2738-2
15. Marchand, L., Grebenshchykova, Z. & Mench, M. (2016). Intra-specific variability of the guaiacol peroxidase (GPOD) activity in roots of Phragmites australis exposed to copper excess. Ecological Engineerin, 90, pp. 57-62. doi: https://doi: 10.1016/ j.ecoleng.2016.01.055 https://doi.org/10.1016/j.ecoleng.2016.01.055
16. Mika, A. & Lьthje, S. (2003). Properties of guaiacol peroxidase activities isolated from corn (Zea mays L.) root plasma membranes. Plant Physiol., 132, pp. 1489-1498. doi: https://doi: 10.1104/pp.103.020396 https://doi.org/10.1104/pp.103.020396
17. Park, Jong-Sug, Choung, Myoung-Gun, Kim, Jung-Bong, Hahn, Bum-Soo, Kim, Jong-Bum, Bae, Shin-Chul, Roh, Kyung-Hee, Kim, Yong-Hwan, Cheon, Choong-Ill, Sung, Mi-Kyung & Cho, Kang-Jin (2007). Genes up-regulated during red coloration in UV-B irradiated lettuce leaves. Plant Cell Rep., 26, pp. 507-516. https://doi.org/10.1007/s00299-006-0255-x
18. Park, Jong-Sug, Kim, Jung-Bong, Cho, Kang-Jin, Cheon, Choong-Ill, Sung, Mi-Kyung, Choung, Myoung-Gun & Kyung-Hee, Roh. (2008). Arabidopsis R2R3-MYB transcription factor AtMYB60 functions as a transcriptional repressor of anthocyanin biosynthesis in lettuce (Lactuca sativa). Plant Cell Rep., 27, pp. 985-994. https://doi.org/10.1007/s00299-008-0521-1
19. Smirnov, O.E., Kosyan, A.M., Kosyk, O.I. & Taran, N.Yu. (2015). Response of phenolic metabolism induced by aluminium toxicity in Fagopyrum esculentum Moench. plants. Ukr. Biochem. J., 87, No. 6, pp. 129-135. doi: https://doi.org/10.15407/ubj87.06.129
20. Taran, N., Batsmanova, L., Kovalenko, M. & Okanenko, A. (2016). Impact of metal nanoform colloidal solution on the adaptive potential of plants. Nanoscale Res Lett., 11, pp. 89-95. doi: https://doi:10.1186/s11671-016-1294-z https://doi.org/10.1186/s11671-016-1294-z
21. Zucker, M. (1969). Induction of phenylalanine ammonia-lyase in Xanthium leaf disks. Photosynthetic requirement and effect of daylength. Plant Physiol., 44, pp. 912-922. https://doi.org/10.1104/pp.44.6.912