Fiziol. rast. genet. 2018, vol. 50, no. 5, 383-401, doi:

Response of different efficiency symbiotic systems on drought and use of the exogenous lectin as an protector of its negative action

Rybachenko L.I., Kots S.Ya., Melnik V.M., Rybachenko O.R.

  • Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine 31/17 Vasylkivska St., Kyiv, 03022, Ukraine

The response of symbiotic soybean systems created by contrasting strains and Tn5-mutants of Bradyrhizobium japonicum on the effect of insufficient water supply was studied, and the possibility of using exogenous lectin as a drought protector on a symbiotic apparatus of soybean plant has been researched. It was revealed that insufficient water supply had a negative influence on the processes of formation and functioning of soybean—B. japonicum symbiotic systems for all investigated rhizobium strains and Tn5-mutants. It was found that the use of B. japonicum Т21-2 strain for inoculation of soybean seeds allowed to form more effective symbiotic systems under optimal and insufficient water supply of plants compared with inoculation by rhizobia of strain 646. In addition, it was found that under conditions of insufficient moisture of the substrate symbiotic systems created with the T21-2 strain formed a smaller number, however, more active nitrogen-fixing root nodules than other studied strains. It is shown that the use of exogenous lectin at concentration of 100 mg/ml for treatment of soybean seeds activates the functioning of the symbiotic apparatus, increases the activity of ascorbate and guaiacol peroxidase in the roots and nodules of plants irrespective of the level of water supply. Such changes in soybean—B. japonicum systems are likely to indicate their higher stress tolerance to adverse conditions, in particular to insufficient water supply, and allow to considerate of soybean seeds lectin as protector of the adverse effects of drought.

Keywords: Bradyrhizobium japonicum, guaiacol peroxidase, ascorbate peroxidase, nitrogen-fixing activity, legume-rhizobial simbiosis, exogenous lectin, soybean

Fiziol. rast. genet.
2018, vol. 50, no. 5, 383-401

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