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Fiziol. rast. genet. 2017, vol. 49, no. 6, 521-532, doi: https://doi.org/10.15407/frg2017.06.521

FREQUENCY AND SPECTRUM OF MUTATIONS IN FLAX (Linum humile Mill.) UNDER THE ACTION OF NEW DIMETHYL SULPHATE DERIVATIVES

Tigova A.V., Soroka A.I.

  • Institute of Oilseed Crops, National Academy of Agrarian Sciences of Ukraine 1 Instytutska St., Settl. Soniachny, Zaporizhzhia, 69093, Ukraine

The effect of new chemical mutagens DG-2, DG-6, DG-7, DG-9 — derivatives of dimethyl sulfate (DMS) — on the frequency and spectrum of mutations in Linum humile Mill. was studied in the M2 generation. In the M1 generation, in flax varieties Iceberg and Solnechny, the survival of plants and a number of morphometric characteristics were evaluated. It was shown that the DMS derivatives exerted both an inhibitory and stimulating effect on the investigated traits. In the next M2 generation, a preliminary estimation of the frequency and spectrum of induced mutations was made. The final conclusion on the presence of mutations in M2 was made after confirmation of their manifestation in the M3 generation. It was found that the total frequency of mutations caused by DG mutagens significantly exceeded the mutation rate induced by the initial DMS agent, and varied from 1.96 to 20.37 %, depending on the type and concentration of the mutagen. The mutation rate caused by the novel mutagens did not exceed the mutation frequency caused by the EMS mutagen (ethyl methane sulfonate), however those mutagens differed in the spectrum of the induced mutations. Regarding the spectrum of the changes obtained, 27 types of mutations were identified, which were subdivided into five groups, depending on the type and direction of the changes. The increased mutability of the variety Solnechny in comparison with the variety Iceberg was revealed. It was demonstrated that with an increase in the concentration of mutagens, the frequency of mutations increased significantly for both flax varieties.

Keywords: Linum humile Mill., flax, mutagenesis, M1 and M2 generations, dimethyl sulfate, dimethyl sulfate derivatives, ethyl methane sulfonate, mutation, spectrum, frequency

Fiziol. rast. genet.
2017, vol. 49, no. 6, 521-532

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References

1. Vasko, V.A., Gudim, O.V. & Rozhkov, O.G. (2015). Application of experimental mutagenesis in plant breeding. Selektsiya i nasinnitstvo, Iss. 107, pp. 8-18 [in Russian].

2. Kirichenko, V.V. & Vasko, V.O. (2015). Mutant dime dimethyl sulfate for M1 meiosis to sonyashnik. Selektsiya i nasinnitstvo, Iss. 108, pp. 99-105 [in Ukrainian].

3. Korolev, K.P., Bogdan, V.Z. & Bogdan, T.M. (2016). Induced mutagenesis of flax (Linum usitatissimum L.) as a method of creating a new source material for priority areas in breeding. Vestnyk Beloruskoy gosudarstvennoy selskohozyaystvennoy akademii, No. 4, pp. 73-75 [in Russian].

4. Krotova, L.A. (2015). Chemical mutagenesis as a method for creating starting material for breeding soft wheat. Elektronnyiy nauchno-metodicheskiy zhurnal Omskogo gosudarstvennogo agrarnogo universiteta, No. 2 (2), pp. 13-17 [in Russian].

5. Lyah, V.A., Polyakova, I.A. & Soroka, A.I. (2009). Induced mutagenesis of oil seeds. Zaporozhe: Zaporozhe: Izdatelstvo Zaporozhskogo natsionalnogo universiteta [in Russian].

6.Morgun, V.V. & Logvinenko, V.F. Mutational selection of wheat. Kiev: Nauk. dumka [in Russian].

7. Opalko, A.I. & Opalko, O.A. (2012). Іndukovіn mutaciі Rosilin: history and perspectives. Induction mutagenesis in the selection of roslin: Zbirnyk naukovyh prats. Bila Tserkva, pp.38-45 [in Ukrainian].

8. Pirko, I.F. (2003). The use of dimethyl sulfate in the selection of garden chrysanthemums (Chrysanthemum w hortorum Bailey.). Promyishlennaya botanika, Iss. 3, pp. 178-179 [in Russian].

9. Pogreblyuk, M.V., Kulish, O.Yu. & Kovalchuk, Z.V. (2016). Application of the method of experimental mutagenesis for vegetable maize. Faktory eksperimentalnoyi evolyutsiyi organizmiv, 18, pp. 102-105 [in Ukrainian].

10. Polyakova, I.A. & Lyah, V.A. (2002). Induction of variability in M2 in different genotypes of oil flax. Mutation rate. Visnyk ZaporIzkogo natsionalnogo universitetu. Biologichni nauky, 1, pp. 1-5 [in Russian].

11. Chopra, V.L. (2005). Mutagenesis: Investigating the process and processing the outcome for crop improvement. Curr. Sci., 89 (2), pp. 353-359.

12. Deepthi, T. & Remesh, K.N. (2016). Imhact of EMS induction on morphological, anatomical and physiological traits of bhindic Abelmoschus esculentus (L.) Moench. Int. Recent Res. Life Sci., 3, pp. 4-11.

13. Luan, Y.S., Zhang, J., Gao, X.R. & An, L.J. (2007). Mutation induced by ethyl methane sulphonate (Ems), in vitro screening for salt tolerance and plant regeneration of sweet potato (Ipomoea batatas L.). Plant Cell, Tissue Organ Culture, 88 (1), pp. 77-81. https://doi.org/10.1007/s11240-006-9183-2

14. Mahla, H.R., Shekhawat, A. & Kumar, D. (2010). A study on EMS and gamma mutagenesis of clusterbean (Cyamopsis tetragonoloba (L.) Taub.). Plant Mutat. Rep., 2 (2), pp. 28-32.

15. Micke, A., Donini, B. & Maluszynskiy, M. (1990). Induced mutations for crop improvement. Mutat. Breed., 7, pp. 1-41.

16. Raina, A., Laskar, R.A., Khursheed, S., Amin, R., Tantray, Y.R., Parveen, K. & Khan, S. (2016). Role of mutation breeding in crop improvement - past, present and future. Asian Reserch Journal of Agriculture, 2 (2), pp. 1-13. https://doi.org/10.9734/ARJA/2016/29334

17. Rajarajan, D., Saraswathi, R., Sassikumar, D. & Ganesh, S. (2014). Fixation of lethal dose and effect of ethyl methane sulphonate induced mutagenesis in rice Adt (R) 47. Life Sci. Leaflets., 57, pp. 65-72.

18. Talebi, Ali B., Talebi, Amin B., Shahrokhifar, B. (2012). Ethyl methane sulphonate (EMS) induced mutagenesis in malaysian rice (cv. MR219) for lethal dose determination. American Journal of Plant Sciences, 3, 1661-1665. https://doi.org/10.4236/ajps.2012.312202

19. Wasserman, L. (2005). All of statistics: A concise course in statistical inference. Springer.