Fiziol. rast. genet. 2017, vol. 49, no. 4, 293-299, doi: https://doi.org/10.15407/frg2017.04.293

PECULIARITIES OF INHIBITION OF α-AMYLASE FROM WHEAT GRAIN WITH SODIUM PHYTATE

Khakimzhanov A.A., Shansharova D.A., Tilegen B., Kuzovlev V.A.

  • M.A. Aitkhozhin Institute of Molecular Biology and Biochemistry Ministry of Education and Science of the Republic of Kazakhstan 86 Dosmuhamedov St., Almaty, 050012, Kazakhstan
  • Almaty Technological University 100 Tole by St., Almaty, 050012, Kazakhstan

Specific features in the effect of sodium phytate on the activity of the two main isozyme groups of a-amylase of wheat grain are established. Phytate strongly inhibited the activity of high pI a-amylase (group a-AMY1). In contrast, the a-AMY2 (low pI isoenzymes) were weakly suppressed by this compound. The inhibitory effect of phytate on the activity of a-amylase is comparable in level to that of the known chelator of divalent metals, EDTA-Na2. However, in the case of phytate, the inhibitory effect on individual isoenzyme groups was more selective. Possible ways of sodium phytate impact on the activity of wheat a-amylase are discussed.

Keywords: Triticum aestivum L., a-amylase, isozymes, sodium phytate, EDTA, inhibition

Fiziol. rast. genet.
2017, vol. 49, no. 4, 293-299

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