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Fiziol. rast. genet. 2016, vol. 48, no. 5, 444-449, doi: https://doi.org/10.15407/frg2016.05.444

Compatible osmolites proline and sucrose in experimental tobacco plants under effect of lethal water stress

Sergeeva L.E., Kurchii V.M., Bronnikova L.I.

  • Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine 31/17 Vasylkivska St., Kyiv, 03022, Ukraine

The proline level and sucrose/monosaccharides ratio in R1 tobacco plants, obtained from Cd-resistant cell lines were investigated. Those plants challenged the lethal for wild type plants water stress (0,3 M mannitol). The proline level increased, sucrose content and carbohydrate ratio decreased in plants on 5 day of stress pressure. These data reflects the proline key role in plants, cultivated under permanent stress conditions. But the proline content reduced and sucrose content and carbohydrate ratio raised in adequate proportions, if water stress increased (0,6 M mannitol). The osmolytes developed common protective role under those conditions. Stress tolerance was detected for R1 plants during recovery period too.

Keywords: tobacco, cell selection, cadmium cations, water deficit, tolerance, proline, sucrose, monosaccharides

Fiziol. rast. genet.
2016, vol. 48, no. 5, 444-449

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1. Andriushchenko, V.K., Saianova, V.V., Zhuchenko, A.A., Diachenko N.I., Chilikina, L.A., Drozdov, V.V., Korochkina, S.K., Cherep, L.A., Medvedev, V.V. & Niutin, Yu.I. (1981). The modification of the proline evaluation method for the detection of drought tolerant forms of Lycopersicon Tourn. Izv. of Moldav. SSR acad. nauk., No. 4, pp. 55-60 [in Russian].

2. Sakalo, V.D. & Kurchii, V.M. (2009). Activity of sucrose synthase and invertase in the maize etiolated seedlings under action of stress factors. Fisiologiya i biokhimiya kult.. rastenij, 41, No. 5, pp. 400-407 [in Ukrainian].

3. Sakalo, V.D., Larchenko, K.A. & Kurchii, V.M. (2009). Synthesis and sucrose metabolism in the leaves of maize seedlings under water stress. Fisiologiya i biokhimiya kult.. rastenij, 41, No. 4, pp. 305-313 [in Ukrainian].

4. Sergeeva, L.E. (1991). The investigation of tobacco cell lines resistant to salt and water stresses and their regenerants. (Extended abstract of candidate thesis). Institute of Plant Physiology and Genetics NAS Ukraine, Kiev, Ukraine [in Russian].

5. Sergeeva, L.E. (2013). Cell selection with heavy metal ions for obtaining plant genotypes with combined resistance to abiotic stresses. Kiev: Logos [in Russian].

6. Battaglia, M., Solorzano, R.M., Hernandez, M., Cuellar-Ortiz, S., García-Gomez, B., Marquez, J. & Covarrubias, A.A. (2007). Proline-rich cell wall proteins accumulate in growing regions and phloem tissue in response to water deficit in common bean seedlings. Planta, 225, pp. 1121-1133. https://doi.org/10.1007/s00425-006-0423-9

7. Boscaiu, M., Esperanza, M., Fola, O. & Scridon, S. (2009). Osmolyte accumulation in xerophytes as a response to environmental stress. Bul. Univ. Agr. Sci and Vet Med. Cluj-Napoca Hort., 66, pp. 96-102.

8. Kiyosue, T., Yoshiba, Y., Yamaguchi-Shinozaki, K. & Shinozaki, K. (1996). A nuclear gene encoding mitochondrial proline dehydrogenase an enzyme involved in proline metabolism, up regulated by proline but down regulated by dehydration in Arabidopsis. Plant Cell, 8, pp. 1323-335. https://doi.org/10.1105/tpc.8.8.1323

9. Showalter, A.M., Keppler, B., Lichtenberg, J. & Dazhang, Gu. (2010). A bioinformatics approach to the identification, classification and analysis of hydroxyproline-rich glycoproteins. Plant Physiol., 153, pp. 485-513. https://doi.org/10.1104/pp.110.156554

10. Stein, H., Honig, A., Miller, G., Erster, O., Eilenberg, H., Csonka, L.N., Szabados, L., Koncz, C. & Zilberstein, A. (2011). Elevation of free proline and proline-rich protein levels by simultaneous manipulations of proline biosynthesis and degradation in plants. Plant Sci, 181, pp. 140-150. https://doi.org/10.1016/j.plantsci.2011.04.013

11. Szabados, L. & Savoure, A. (2010). Proline: a multifunctional amino acid. Trends Plant Sci., 15, pp. 89-97. https://doi.org/10.1016/j.tplants.2009.11.009

12. Vertucci, C.W. & Leopold, A.C. (1987). The relationship between water binding and desiccation tolerance in tissues. Plant Physiol., 85, pp. 232-238. https://doi.org/10.1104/pp.85.1.232

13. Wang, L., Zhang, L., Chen, G. & Li, X. (2005). Physiological responses of sweet potato callus to drought and salt stress. Shengtaixue zazhi = Ch. J. Ecol. Bot., 25, pp. 1508-1514.

14. Xiong, L., Wang, R.-G., Mao, G. & Koczan, J.M. (2006). Identification of drought tolerance determinants by genetic analysis of root response to drought stress and abscisic acid. Plant Physiol., 142, pp. 1065-1074. https://doi.org/10.1104/pp.106.084632